cDNA Clone DDX20

• Plasmid: pCMV-FLAG DDX20 1-716
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG-DDX20 1-716
• Genbank: AAH34953.1
• Tag: FLAG
• Species: Human

Sequence of Insert

AGATCTATGGCGGCGGCATTTGAAGCCTCGGGAGCCTTAGCAGCAGTGGC
GACTGCTATGCCGGCTGAGCATGTGGCCGTGCAGGTCCCGGCCCCAGAGC
CAACACCCGGGCCTGTGAGGATCCTGCGGACCGCTCAGGATCTCAGCAGC
CCGCGGACCCGCACGGGGGATGTGCTGTTGGCGGAGCCGGCCGACTTCGA
GTCACTGCTGCTTTCGCGGCCGGTGCTGGAGGGGCTGCGGGCGGCCGGCT
TCGAGAGGCCCTCGCCGGTGCAGCTCAAGGCCATCCCGCTGGGGCGCTGC
GGGCTCGATTTAATTGTTCAAGCTAAATCTGGCACCGGGAAAACCTGTGT
GTTCTCCACCATAGCTTTGGACTCTCTTGTTCTTGAAAACTTAAGTACCC
AGATTTTGATCTTGGCTCCTACAAGAGAAATTGCTGTACAGATACATTCT
GTTATTACAGCCATTGGAATAAAAATGGAAGGCTTAGAGTGTCATGTCTT
TATTGGAGGGACCCCATTATCACAAGACAAAACCAGACTTAAAAAGTGTC
ATATTGCTGTTGGATCTCCTGGCAGAATTAAGCAACTCATAGAACTTGAC
TACTTGAACCCAGGCAGTATACGCCTCTTTATTCTTGATGAAGCAGATAA
GCTTTTAGAAGAAGGCAGCTTCCAGGAGCAAATAAATTGGATTTATTCTT
CCTTGCCTGCCAGTAAACAGATGCTGGCAGTATCAGCTACTTATCCCGAA
TTTTTGGCTAATGCTTTGACAAAGTACATGAGAGATCCCACTTTTGTAAG
ACTGAATTCCAGTGATCCAAGTCTCATAGGTTTGAAGCAGTATTACAAAG
TTGTCAATTCATACCCTTTGGCACATAAGGTTTTTGAGGAAAAGACTCAG
CATTTACAGGAACTGTTCAGCAGAATTCCATTTAATCAAGCTTTAGTCTT
TTCTAATTTGCACAGCAGAGCACAACATTTGGCTGATATCCTTTCTTCTA
AAGGCTTTCCTGCTGAGTGCATTTCAGGCAATATGAATCAGAATCAGCGT
CTTGATGCTATGGCTAAACTGAAGCACTTTCATTGCAGAGTCCTCATTTC
CACAGATTTGACTTCTCGTGGGATTGATGCTGAGAAGGTGAATCTGGTTG
TAAATCTGGATGTACCATTGGATTGGGAGACATACATGCATCGGATTGGG
AGAGCTGGCCGTTTTGGTACATTGGGGCTGACAGTGACCTACTGTTGCCG
GGGAGAGGAAGGAAATATGATGATGAGAATTGCCCAGAAATGTAATATCA
ACCTTCTCCCTTTACCAGATCCCATTCCTTCTGGTCTGATGGAAGAATGT
GTGGATTGGGATGTGGAAGTTAAAGCTGCTGTGCATACATATGGTATAGC
AAGTGTACCTAACCAACCCTTAAAAAAGCAAATTCAGAAAATAGAGAGAA
CCCTTCAAATTCAGAAAGCTCATGGTGACCACATGGCTTCCTCTAGAAAT
AATTCTGTATCTGGACTATCAGTCAAATCAAAAAATAATACCAAACAAAA
GCTTCCTGTGAAAAGCCACTCAGAATGTGGAATCATAGAAAAAGCAACGT
CACCAAAAGAACTGGGCTGTGACAGGCAATCCGAAGAGCAAATGAAGAAT
TCTGTTCAGACTCCCGTTGAAAACTCCACCAACAGTCAGCACCAGGTCAA
AGAAGCTTTACCTGTGTCACTCCCCCAGATTCCTTGTCTGTCTTCCTTTA
AAATCCATCAGCCATACACGTTGACTTTTGCTGAATTGGTAGAGGATTAT
GAACATTATATTAAAGAGGGGTTAGAGAAACCTGTGGAAATCATCAGGCA
CTACACAGGCCCTGGGGATCAGACTGTGAATCCTCAAAATGGTTTTGTGA
GAAATAAAGTTACTGAACAGAGAGTCCCTGTATTGGCAAGTAGTAGCCAA
TCTGGAGACTCTGAGAGTGACAGTGATTCTTACAGCTCAAGAACCTCTTC
CCAGAGCAAAGGAAATAAGTCATACTTGGAAGGCTCTTCTGATAATCAGC
TGAAAGACTCTGAATCTACGCCTGTGGATGATCGTATTTCTTTGGAACAA
CCACCAAATGGAAGTGACACCCCCAATCCAGAGAAATATCAAGAATCACC
TGGGTAACAGATGAAGACAAGACTTAAAGAGGGGGCTAGCCAGAGAGCTA
AGCAGAGCCGGAGAAACCTACCCAGGCGGTCTTCCTTCAGATTGCAGACT
GAAGCCCAGGAAGATGATTGGTATGACTGTCATAGGGAAATACGTCTGAG
TTTTTCTGATACCTATCAGGATTATGAGGAGTACTGGAGAGCTTACTACA
GGGCATGGCAAGAATATTATGCTGCCGCTTCTCATTCATATTATTGGAAT
GCTCAGAGACATCCAAGTTGGATGGCAGCTTATCACATGAATACCATTTA
TCTACAAGAAATGATGCATAGTAACCAGTGACTCGAG

Amino Acid Sequence

MDYKDDDDKGSMAAAFEASGALAAVATAMPAEHVAVQVPAPEPTPGPVRI
LRTAQDLSSPRTRTGDVLLAEPADFESLLLSRPVLEGLRAAGFERPSPVQ
LKAIPLGRCGLDLIVQAKSGTGKTCVFSTIALDSLVLENLSTQILILAPT
REIAVQIHSVITAIGIKMEGLECHVFIGGTPLSQDKTRLKKCHIAVGSPG
RIKQLIELDYLNPGSIRLFILDEADKLLEEGSFQEQINWIYSSLPASKQM
LAVSATYPEFLANALTKYMRDPTFVRLNSSDPSLIGLKQYYKVVNSYPLA
HKVFEEKTQHLQELFSRIPFNQALVFSNLHSRAQHLADILSSKGFPAECI
SGNMNQNQRLDAMAKLKHFHCRVLISTDLTSRGIDAEKVNLVVNLDVPLD
WETYMHRIGRAGRFGTLGLTVTYCCRGEEGNMMMRIAQKCNINLLPLPDP
IPSGLMEECVDWDVEVKAAVHTYGIASVPNQPLKKQIQKIERTLQIQKAH
GDHMASSRNNSVSGLSVKSKNNTKQKLPVKSHSECGIIEKATSPKELGCD
RQSEEQMKNSVQTPVENSTNSQHQVKEALPVSLPQIPCLSSFKIHQPYTL
TFAELVEDYEHYIKEGLEKPVEIIRHYTGPGDQTVNPQNGFVRNKVTEQR
VPVLASSSQSGDSESDSDSYSSRTSSQSKGNKSYLEGSSDNQLKDSESTP
VDDRISLEQPPNGSDTPNPEKYQESPG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8968
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter FLAG for mammalian expression.; ; Estimated MW of expressed protein : 79977 Daltons