cDNA Clone LIMK2

• Plasmid: pEGFP-C2-LIMK2
• Parent Plasmid: pEGFPC2
• Expressed: GFP-LIMK2
• Genbank: D45906
• Tag: GFP
• Species: Human

Sequence of Insert

GAATTCTCCGCGCTGGCGGGTGAAGATGTCTGGAGGTGTCCAGGCTGTGG
GGACCACATTGCTCCAAGCCAGATATGGTACAGGACTGTCAACGAAACCT
GGCACGGCTCTTGCTTCCGGTGTTCAGAATGCCAGGATTCCCTCACCAAC
TGGTACTATGAGAAGGATGGGAAGCTCTACTGCCCCAAGGACTACTGGGG
GAAGTTTGGGGAGTTCTGTCATGGGTGCTCCCTGCTGATGACAGGGCCTT
TTATGGTGGCTGGGGAGTTCAAGTACCACCCAGAGTGCTTTGCCTGTATG
AGCTGCAAGGTGATCATTGAGGATGGGGATGCATATGCACTGGTGCAGCA
TGCCACCCTCTACTGTGGGAAGTGCCACAATGAGGTGGTGCTGGCACCCA
TGTTTGAGAGACTCTCCACAGAGTCTGTTCAGGAGCAGCTGCCCTACTCT
GTCACGCTCATCTCCATGCCGGCCACCACTGAAGGCAGGCGGGGCTTCTC
CGTGTCCGTGGAGAGTGCCTGCTCCAACTACGCCACCACTGTGCAAGTGA
AAGAGGTCAACCGGATGCACATCAGTCCCAACAATCGAAACGCCATCCAC
CCTGGGGACCGCATCCTGGAGATCAATGGGACCCCCGTCCGCACACTTCG
AGTGGAGGAGGTGGAGGATGCAATTAGCCAGACGAGCCAGACACTTCAGC
TGTTGATTGAACATGACCCCGTCTCCCAACGCCTGGACCAGCTGCGGCTG
GAGGCCCGGCTCGCTCCTCACATGCAGAATGCCGGACACCCCCACGCCCT
CAGCACCCTGGACACCAAGGAGAATCTGGAGGGGACACTGAGGAGACGTT
CCCTAAGGCGCAGTAACAGTATCTCCAAGTCCCCTGGCCCCAGCTCCCCA
AAGGAGCCCCTGCTGTTCAGCCGTGACATCAGCCGCTCAGAATCCCTTCG
TTGTTCCAGCAGCTATTCACAGCAGATCTTCCGGCCCTGTGACCTAATCC
ATGGGGAGGTCCTGGGGAAGGGCTTCTTTGGGCAGGCTATCAAGGTGACA
CACAAAGCCACGGGCAAAGTGATGGTCATGAAAGAGTTAATTCGATGTGA
TGAGGAGACCCAGAAAACTTTTCTGACTGAGGTGAAAGTGATGCGCAGCC
TGGACCACCCCAATGTGCTCAAGTTCATTGGTGTGCTGTACAAGGATAAG
AAGCTGAACCTGCTGACAGAGTACATTGAGGGGGGCACACTGAAGGACTT
TCTGCGCAGTATGGATCCGTTCCCCTGGCAGCAGAAGGTCAGGTTTGCCA
AAGGAATCGCCTCCGGAATGGCCTATTTGCACTCTATGTGCATCATCCAC
CGGGATCTGAACTCGCACAACTGCCTCATCAAGTTGGACAAGACTGTGGT
GGTGGCAGACTTTGGGCTGTCACGGCTCATAGTGGAAGAGAGGAAAAGGG
CCCCCATGGAGAAGGCCACCACCAAGAAACGCACCTTGCGCAAGAACGAC
CGCAAGAAGCGCTACACGGTGGTGGGAAACCCCTACTGGATGGCCCCTGA
GATGCTGAACGGAAAGAGCTATGATGAGACGGTGGATATCTTCTCCTTTG
GGATCGTTCTCTGTGAGATCATTGGGCAGGTGTATGCAGATCCTGACTGC
CTTCCCCGAACACTGGACTTTGGCCTCAACGTGAAGCTTTTCTGGGAGAA
GTTTGTTCCCACAGATTGTCCCCCGGCCTTCTTCCCGCTGGCCGCCATCT
GCTGCAGACTGGAGCCTGAGAGCAGACCAGCATTCTCGAAATTGGAGGAC
TCCTTTGAGGCCCTCTCCCTGTACCTGGGGGAGCTGGGCATCCCGCTGCC
TGCAGAGCTGGAGGAGTTGGACCACACTGTGAGCATGCAGTACGGCCTGA
CCCGGGACTCACCTCCCTAGGTCGAC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
EFSALAGEDVWRCPGCGDHIAPSQIWYRTVNETWHGSCFRCSECQDSLTN
WYYEKDGKLYCPKDYWGKFGEFCHGCSLLMTGPFMVAGEFKYHPECFACM
SCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQEQLPYS
VTLISMPATTEGRRGFSVSVESACSNYATTVQVKEVNRMHISPNNRNAIH
PGDRILEINGTPVRTLRVEEVEDAISQTSQTLQLLIEHDPVSQRLDQLRL
EARLAPHMQNAGHPHALSTLDTKENLEGTLRRRSLRRSNSISKSPGPSSP
KEPLLFSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVT
HKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDK
KLNLLTEYIEGGTLKDFLRSMDPFPWQQKVRFAKGIASGMAYLHSMCIIH
RDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRAPMEKATTKKRTLRKND
RKKRYTVVGNPYWMAPEMLNGKSYDETVDIFSFGIVLCEIIGQVYADPDC
LPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCRLEPESRPAFSKLED
SFEALSLYLGELGIPLPAELEELDHTVSMQYGLTRDSPP*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU8942
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GFP for mammalian expression.; ; Estimated MW of expressed protein : 100535 Daltons