cDNA Clone PPP4R2

• Plasmid: pEBG6P-PPP4R2 FL
• Parent Plasmid: pEBG6P
• Expressed: GST-PPP4R2 FL
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGTGCCAGGCGCCATGTTGGAGGGCTGGTGGTAGCGGCTT
GGGGAGGTGCTCACTCTGTCGGTCTTGCTCTCTCGCACGCTTCCCCCGGC
TCCCTTCGTTTCCCCCCCCCGGTCGCCTGCGTGCCGGAGTGTGTGCGAGG
GAGGGGGAGGGCGTCGGGGGGGTGGGGGGAGGCGTTCCGGTCCCCAAAAG
ACCCGCGGAGGGAGGCGGAGGCTGTGAGGGACTCCGGGAAGCCATGGACG
TCGAGAGGCTCCAGGAGGCGCTGAAAGATTTTGAGAAGAGGGGGAAAAAG
GAAGTTTGTCCTGTCCTGGATCAGTTTCTTTGTCATGTAGCCAAGACTGG
AGAAACAATGATTCAGTGGTCCCAATTTAAAGGCTATTTTATTTTCAAAC
TGGAGAAAGTGATGGATGATTTCAGAACTTCAGCTCCTGAGCCAAGAGGT
CCTCCCAACCCTAATGTCGAATATATTCCCTTTGATGAAATGAAGGAAAG
AATACTGAAAATTGTCACTGGATTTAATGGTATCCCTTTTACTATTCAGC
GACTATGTGAATTGTTAACAGATCCAAGGAGAAACTATACAGGAACAGAC
AAATTTCTCAGAGGAGTAGAAAAGAATGTGATGGTTGTTAGCTGTGTTTA
TCCTTCTTCAGAGAAAAACAATTCCAATAGTTTAAATCGAATGAATGGTG
TTATGTTTCCTGGAAATTCACCAAGCTATACTGAGAGGTCTAATATAAAT
GGGCCTGGGACACCCAGGCCACTTAATCGACCAAAGGTTTCTTTGTCAGC
CCCCATGACAACAAATGGGTTGCCTGAGAGCACAGACAGCAAAGAGGCAA
ATTTGCAGCAAAATGAGGAGAAAAATCACAGTGACTCTTCGACCTCTGAA
TCAGAAGTTTCCTCAGTGAGCCCTTTGAAAAATAAACATCCAGATGAAGA
TGCTGTGGAAGCTGAGGGGCATGAGGTAAAAAGACTCAGGTTTGACAAAG
AAGGCGAAGTCAGAGAAACAGCCAGTCAAACGACTTCCAGCGAAATTTCT
TCAGTTATGGTAGGAGAAACAGAAGCATCATCTTCATCTCAGGATAAAGA
CAAAGATAGCCGTTGTACCCGGCAGCACTGTACAGAAGAGGATGAAGAAG
AGGATGAAGAGGAAGAAGAAGAGTCTTTTATGACATCAAGAGAAATGATC
CCAGAAAGAAAAAATCAAGAAAAAGAATCTGATGATGCCTTAACTGTGAA
TGAAGAGACTTCTGAGGAAAATAATCAAATGGAGGAATCTGATGTGTCTC
AAGCTGAGAAAGATTTGCTACATTCTGAAGGTAGTGAAAACGAAGGCCCT
GTAAGTAGTAGTTCTTCTGACTGCCGTGAAACAGAAGAATTAGTAGGATC
CAATTCCAGTAAAACTGGAGAGATTCTTTCAGAATCATCCATGGAAAATG
ATGACGAAGCCACAGAAGTCACCGATGAACCAATGGAACAAGACTAAGCG
GCCGCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMCQA
PCWRAGGSGLGRCSLCRSCSLARFPRLPSFPPPGRLRAGVCAREGEGVGG
VGGGVPVPKRPAEGGGGCEGLREAMDVERLQEALKDFEKRGKKEVCPVLD
QFLCHVAKTGETMIQWSQFKGYFIFKLEKVMDDFRTSAPEPRGPPNPNVE
YIPFDEMKERILKIVTGFNGIPFTIQRLCELLTDPRRNYTGTDKFLRGVE
KNVMVVSCVYPSSEKNNSNSLNRMNGVMFPGNSPSYTERSNINGPGTPRP
LNRPKVSLSAPMTTNGLPESTDSKEANLQQNEEKNHSDSSTSESEVSSVS
PLKNKHPDEDAVEAEGHEVKRLRFDKEGEVRETASQTTSSEISSVMVGET
EASSSSQDKDKDSRCTRQHCTEEDEEEDEEEEEESFMTSREMIPERKNQE
KESDDALTVNEETSEENNQMEESDVSQAEKDLLHSEGSENEGPVSSSSSD
CRETEELVGSNSSKTGEILSESSMENDDEATEVTDEPMEQD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8937
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 82908 Daltons