cDNA Clone HEPS HEPt

• Plasmid: pETDuet Bacillus HEPS 6His HEPT
• Parent Plasmid: pETDuet1
• Genbank: P31112 and P31114
• Tag: 6His
• Species: Bacillus subtilis

Sequence of Insert

DNA sequence HEPS insert :
CATATGTTGCAAGACATCTACGGAACTTTAGCCAATCTGAACACGAAATT
AAAACAAAAGCTGTCTCATCCTTATTTAGCGAAGCATATTTCTGCGCCGA
AAATTGATGAGGATAAGCTTCTTCTTTTTCATGCTTTATTTGAAGAAGCC
GACATAAAAAACAACGACAGAGAAAATTATATTGTAACAGCGATGCTTGT
ACAAAGCGCCCTTGATACCCATGATGAAGTGACGACAGCTAGAGTCATAA
AACGAGACGAAAACAAAAACCGCCAATTGACTGTTCTCGCGGGCGATTAT
TTCAGCGGGCTGTACTACTCTTTACTATCTGAAATGAAGGATATCTACAT
GATTCGGACGCTTGCTACAGCCATTAAAGAAATCAACGAACATAAAATTC
GTCTGTATGACCGTTCTTTCAAGGACGAAAACGATTTTTTCGAAAGTGTC
GGCATCGTTGAATCAGCTTTATTCCATCGTGTGGCGGAACACTTCAACCT
CCCGCGCTGGAAAAAGCTGTCGAGTGATTTTTTTGTATTTAAGCGGCTTA
TGAACGGAAATGATGCATTTCTGGATGTGATCGGCAGTTTTATACAGCTG
GGAAAAACAAAAGAAGAGATATTAGAAGATTGTTTTAAAAAAGCGAAAAA
CAGCATTGAGTCACTTCTGCCTCTAAATTCACCTATTCAGAACATTTTAA
TAAACCGTCTGAAGACAATCAGCCAAGATCAAACCTATCATCAGAAAGTG
GAAGAAGGGTAAGGTACC

DNA sequence HEPT insert : GAATTCGATGTTAAATATCATTCGTTTACTGGCGGAGTCGCTGCCACGCA
TATCGGATGGAAATGAAAACACAGATGTTTGGGTGAATGATATGAAATTT
AAAATGGCCTACTCTTTTTTAAATGACGATATTGATGTAATCGAAAGAGA
ACTTGAACAAACCGTACGTTCCGATTACCCGCTTTTAAGCGAGGCAGGTC
TTCACCTGCTGCAGGCCGGAGGGAAACGTATTCGGCCTGTTTTCGTGCTG
CTTTCTGGCATGTTTGGCGATTATGATATTAATAAGATTAAATATGTCGC
CGTCACTCTGGAAATGATTCACATGGCATCTTTGGTTCATGATGATGTCA
TTGATGATGCAGAGCTTCGCCGAGGAAAACCGACAATCAAAGCAAAGTGG
GACAATCGTATTGCGATGTACACAGGCGATTATATGCTTGCGGGATCTCT
TGAAATGATGACGAGAATTAACGAACCGAAAGCCCATAGGATTTTGTCAC
AGACGATCGTTGAAGTTTGTCTAGGGGAAATTGAGCAGATCAAAGACAAA
TACAACATGGAACAAAATCTCAGAACGTATCTCCGCCGTATCAAAAGAAA
AACAGCTCTCTTGATCGCGGTCAGCTGCCAGCTTGGTGCCATTGCGTCTG
GAGCTGATGAGAAGATTCATAAGGCATTGTACTGGTTTGGGTATTACGTC
GGCATGTCTTATCAGATTATTGATGATATTCTTGATTTTACTTCAACTGA
GGAAGAGCTGGGTAAACCCGTAGGAGGAGATTTGCTTCAAGGAAACGTCA
CATTGCCAGTGCTGTATGCCCTGAAAAATCCTGCATTAAAAAACCAGCTT
AAATTGATTAACAGTGAGACAACGCAGGAACAGCTTGAACCAATCATTGA
AGAAATCAAAAAAACAGATGCAATTGAAGCATCTATGGCAGTAAGCGAAA
TGTATCTGCAGAAAGCTTTTCAGAAATTAAACACGCTTCCTCGAGGGCGC
GCACGCTCGTCTCTTGCAGCCATCGCAAAATATATCGGTAAAAGAAAATT
TTAAGCGGCCGC

Amino Acid Sequence

Protein sequence of HEPS :
MLQDIYGTLANLNTKLKQKLSHPYLAKHISAPKIDEDKLLLFHALFEEAD
IKNNDRENYIVTAMLVQSALDTHDEVTTARVIKRDENKNRQLTVLAGDYF
SGLYYSLLSEMKDIYMIRTLATAIKEINEHKIRLYDRSFKDENDFFESVG
IVESALFHRVAEHFNLPRWKKLSSDFFVFKRLMNGNDAFLDVIGSFIQLG
KTKEEILEDCFKKAKNSIESLLPLNSPIQNILINRLKTISQDQTYHQKVE
EG*

Protein sequence of HEPT :
MGSHHHHHHSQDPNSMLNIIRLLAESLPRISDGNENTDVWVNDMKFKMAY
SFLNDDIDVIERELEQTVRSDYPLLSEAGLHLLQAGGKRIRPVFVLLSGM
FGDYDINKIKYVAVTLEMIHMASLVHDDVIDDAELRRGKPTIKAKWDNRI
AMYTGDYMLAGSLEMMTRINEPKAHRILSQTIVEVCLGEIEQIKDKYNME
QNLRTYLRRIKRKTALLIAVSCQLGAIASGADEKIHKALYWFGYYVGMSY
QIIDDILDFTSTEEELGKPVGGDLLQGNVTLPVLYALKNPALKNQLKLIN
SETTQEQLEPIIEEIKKTDAIEASMAVSEMYLQKAFQKLNTLPRGRARSS
LAAIAKYIGKRKF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8874
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Dual expression vector for bacterial expression.; ; Estimated MW of expressed HEPS : 29234 Daltons; ; Estimated MW of expressed HEPT : 41241 Daltons