cDNA Clone Beclin1

• Synonyms: BECN1
• Plasmid: pFastbac GST Beclin1
• Parent Plasmid: pFastbac GST
• Expressed: GST Beclin1
• Genbank: NP_003757.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAAGGGTCTAAGACGTCCAACAACAGCACCATGCAGGTGAG
CTTCGTGTGCCAGCGCTGCAGCCAGCCCCTGAAACTGGACACGAGTTTCA
AGATCCTGGACCGTGTCACCATCCAGGAACTCACAGCTCCATTACTTACC
ACAGCCCAGGCGAAACCAGGAGAGACCCAGGAGGAAGAGACTAACTCAGG
AGAGGAGCCATTTATTGAAACTCCTCGCCAGGATGGTGTCTCTCGCAGAT
TCATCCCCCCAGCCAGGATGATGTCCACAGAAAGTGCCAACAGCTTCACT
CTGATTGGGGAGGCATCTGATGGCGGCACCATGGAGAACCTCAGCCGAAG
ACTGAAGGTCACTGGGGACCTTTTTGACATCATGTCGGGCCAGACAGATG
TGGATCACCCACTCTGTGAGGAATGCACAGATACTCTTTTAGACCAGCTG
GACACTCAGCTCAACGTCACTGAAAATGAGTGTCAGAACTACAAACGCTG
TTTGGAGATCTTAGAGCAAATGAATGAGGATGACAGTGAACAGTTACAGA
TGGAGCTAAAGGAGCTGGCACTAGAGGAGGAGAGGCTGATCCAGGAGCTG
GAAGACGTGGAAAAGAACCGCAAGATAGTGGCAGAAAATCTCGAGAAGGT
CCAGGCTGAGGCTGAGAGACTGGATCAGGAGGAAGCTCAGTATCAGAGAG
AATACAGTGAATTTAAACGACAGCAGCTGGAGCTGGATGATGAGCTGAAG
AGTGTTGAAAACCAGATGCGTTATGCCCAGACGCAGCTGGATAAGCTGAA
GAAAACCAACGTCTTTAATGCAACCTTCCACATCTGGCACAGTGGACAGT
TTGGCACAATCAATAACTTCAGGCTGGGTCGCCTGCCCAGTGTTCCCGTG
GAATGGAATGAGATTAATGCTGCTTGGGGCCAGACTGTGTTGCTGCTCCA
TGCTCTGGCCAATAAGATGGGTCTGAAATTTCAGAGATACCGACTTGTTC
CTTACGGAAACCATTCATATCTGGAGTCTCTGACAGACAAATCTAAGGAG
CTGCCGTTATACTGTTCTGGGGGGTTGCGGTTTTTCTGGGACAACAAGTT
TGACCATGCAATGGTGGCTTTCCTGGACTGTGTGCAGCAGTTCAAAGAAG
AGGTTGAGAAAGGCGAGACACGTTTTTGTCTTCCCTACAGGATGGATGTG
GAGAAAGGCAAGATTGAAGACACAGGAGGCAGTGGCGGCTCCTATTCCAT
CAAAACCCAGTTTAACTCTGAGGAGCAGTGGACAAAAGCTCTCAAGTTCA
TGCTGACGAATCTTAAGTGGGGTCTTGCTTGGGTGTCCTCACAATTTTAT
AACAAATGAGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMEGSKTSNNSTMQVSFVCQ
RCSQPLKLDTSFKILDRVTIQELTAPLLTTAQAKPGETQEEETNSGEEPF
IETPRQDGVSRRFIPPARMMSTESANSFTLIGEASDGGTMENLSRRLKVT
GDLFDIMSGQTDVDHPLCEECTDTLLDQLDTQLNVTENECQNYKRCLEIL
EQMNEDDSEQLQMELKELALEEERLIQELEDVEKNRKIVAENLEKVQAEA
ERLDQEEAQYQREYSEFKRQQLELDDELKSVENQMRYAQTQLDKLKKTNV
FNATFHIWHSGQFGTINNFRLGRLPSVPVEWNEINAAWGQTVLLLHALAN
KMGLKFQRYRLVPYGNHSYLESLTDKSKELPLYCSGGLRFFWDNKFDHAM
VAFLDCVQQFKEEVEKGETRFCLPYRMDVEKGKIEDTGGSGGSYSIKTQF
NSEEQWTKALKFMLTNLKWGLAWVSSQFYNK*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU8850
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for baculovirus expression.; ; Estimated MW of expressed protein : 78718 Daltons