cDNA Clone TCII

• Plasmid: pGEX6P-1-TCII
• Parent Plasmid: pGEX6P1
• Expressed: GST-TCII
• Genbank: CAK54921.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGAGGCACCTTGGGGCCTTCCTCTTCCTTCTGGGGGTCCTGGG
GGCCCTCACTGAGATGTGTGAAATACCAGAGATGGACAGCCATCTGGTAG
AGAAGTTGGGCCAGCACCTCTTACCTTGGATGGACCGGCTTTCCCTGGAG
CACTTGAACCCCAGCATCTATGTGGGCCTACGCCTCTCCAGTCTGCAGGC
TGGGACCAAGGAAGACCTCTACCTGCACAGCCTCAAGCTTGGTTACCAGC
AGTGCCTCCTAGGGTCTGCCTTCAGCGAGGATGACAGTGACTGCCAGGGC
AAGCCTTCCATGGGCCAGCTGGCCCTCTACCTGCTCGCTCTCAGAGCCAA
CTGTGAGTTTGTCAGGGGCCACAAGGGGGACAGGCTGGTCTCACAGCTCA
AATGGTTCCTGGAGGATGAGAAGAGAGCCATTGGGCATGATCACAAGGGC
CACCCCCACACTAGCTACTACCAGTATGGCCTGTGCATTCTGGCCCTGTG
TCTCCACCAGAAGCGGGTCCATGACAGCGTGGTGGACAAACTTCTGTATG
CTGTGGAACCTTTCCACCAGGGCCACCATTCTGTGGACACAGCAGCCATG
GCAGGCTTGGCATTCACCTGTCTGAAGCGCTCAAACTTCAACCCTGGTCG
GAGACAACGGATCACCATGGCCATCAGAACAGTGCGAGAGGAGATCTTGA
AGGCCCAGACCCCCGAGGGCCACTTTGGGAATGTCTACAGCACCCCATTG
GCATTACAGTTCCTCATGACTTCCCCCATGCCTGGGGCAGAACTGGGAAC
AGCATGTCTCAAGGCGAGGGTTGCTTTGCTGGCCAGTCTGCAGGATGGAG
CCTTCCAGAATGCTCTCATGATTTCCCAGCTGCTGCCCGTTCTGAACCAC
AAGACCTACATTGATCTGATCTTCCCAGACTGTCTGGCACCACGAGTCAT
GTTGGAACCAGCTGCTGAGACCATTCCTCAGACCCAAGAGATCATCAGTG
TCACGCTGCAGGTGCTTAGTCTCTTGCCGCCGTACAGACAGTCCATCTCT
GTTCTGGCCGGGTCCACCGTGGAAGATGTCCTGAAGAAGGCCCATGAGTT
AGGAGGATTCACATATGAAACACAGGCCTCCTTGTCAGGCCCCTACTTAA
CCTCCGTGATGGGGAAAGCGGCCGGAGAAAGGGAGTTCTGGCAGCTTCTC
CGAGACCCCAACACCCCACTGTTGCAAGGTATTGCTGACTACAGACCCAA
GGATGGAGAAACCATTGAGCTGAGGCTGGTTAGCTGGTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMRHLGAFLFLLGVLGALTE
MCEIPEMDSHLVEKLGQHLLPWMDRLSLEHLNPSIYVGLRLSSLQAGTKE
DLYLHSLKLGYQQCLLGSAFSEDDSDCQGKPSMGQLALYLLALRANCEFV
RGHKGDRLVSQLKWFLEDEKRAIGHDHKGHPHTSYYQYGLGILALCLHQK
RVHDSVVDKLLYAVEPFHQGHHSVDTAAMAGLAFTCLKRSNFNPGRRQRI
TMAIRTVREEILKAQTPEGHFGNVYSTPLALQFLMTSPMPGAELGTACLK
ARVALLASLQDGAFQNALMISQLLPVLNHKTYIDLIFPDCLAPRVMLEPA
AETIPQTQEIISVTLQVLSLLPPYRQSISVLAGSTVEDVLKKAHELGGFT
YETQASLSGPYLTSVMGKAAGEREFWQLLRDPNTPLLQGIADYRPKDGET
IELRLVSW*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8834
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression. Differs to genbank at G94S. (as supplied by external customer); ; Estimated MW of expressed protein : 74327 Daltons