cDNA Clone RIPK2

• Plasmid: pLent TAp RIPK2
• Parent Plasmid: pLent TAP
• Expressed: GFP-TAP-RIPK2
• Genbank: NP_003812
• Tag: GFP
• Tag: TAP
• Species: Human

Sequence of Insert

ACTAGTGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGT
GCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCG
TGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAG
TTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGAC
CACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGA
AGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAG
CGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGT
GAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCG
ACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTAC
AACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAA
GGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCG
CCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTG
CCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAA
CGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGA
TCACTCTCGGCATGGACGAGCTGTACAAGTCCGGCCGGACTCAGATCTCG
AGCTCAAGCTTCGATATCGCCCTTGCCACCATGGACACAAGTGCCCACGA
TGAAGCCGTAGACAACAAATTCAACAAAGAACAACAAAACGCGTTCTATG
AGATCTTACATTTACCTAACTTAAACGAAGAACAACGAAACGCCTTCATC
CAAAGTTTAAAAGATGACCCAAGCCAAAGCGCTAACCTTTTAGCAGAAGC
TAAAAAGCTAAATGATGCTCAGGCGCCGAAAGTAGACAACAAATTCAACA
AAGAACGACAAAACGCGTTCTATGAGATCTTACATTTACCTAACTTAAAC
GAAGAACAACGAAACGCCTTCATCCAAAGTTTAAAAGATGACCCAAGCCA
AAGCGCTAACCTTTTAGCAGAAGCTAAAAAGCTAAATGATGCTCAGGCGC
CGAAAGTAGACGCGAGTTGGTCAGCGGGTGCTAGCGAGAACTTGTACTTC
CAGGGCGGAAGATCGATGGAAAAGAGAAGATGGAAAAAGAATTTCATAGC
CGTCTCAGCAGCCAACCGCTTTAAGAAAATCTCATCCTCCGGGGCACTTG
ATTATGATATTCCAACTGCTCTGGAAGTTCTGTTCCAGGGGCCCCTGGGA
TCCAGATCTGGTACCGCGGCCGCCAACGGGGAGGCCATCTGCAGCGCCCT
GCCCACCATTCCCTACCACAAACTCGCCGACCTGCGCTACCTGAGCCGCG
GCGCCTCTGGCACTGTGTCGTCCGCCCGCCACGCAGACTGGCGCGTCCAG
GTGGCCGTGAAGCACCTGCACATCCACACTCCGCTGCTCGACAGTGAAAG
AAAGGATGTCTTAAGAGAAGCTGAAATTTTACACAAAGCTAGATTTAGTT
ACATTCTTCCAATTTTGGGAATTTGCAATGAGCCTGAATTTTTGGGAATA
GTTACTGAATACATGCCAAATGGATCATTAAATGAACTCCTACATAGGAA
AACTGAATATCCTGATGTTGCTTGGCCATTGAGATTTCGCATCCTGCATG
AAATTGCCCTTGGTGTAAATTACCTGCACAATATGACTCCTCCTTTACTT
CATCATGACTTGAAGACTCAGAATATCTTATTGGACAATGAATTTCATGT
TAAGATTGCAGATTTTGGTTTATCAAAGTGGCGCATGATGTCCCTCTCAC
AGTCACGAAGTAGCAAATCTGCACCAGAAGGAGGGACAATTATCTATATG
CCACCTGAAAACTATGAACCTGGACAAAAATCAAGGGCCAGTATCAAGCA
CGATATATATAGCTATGCAGTTATCACATGGGAAGTGTTATCCAGAAAAC
AGCCTTTTGAAGATGTCACCAATCCTTTGCAGATAATGTATAGTGTGTCA
CAAGGACATCGACCTGTTATTAATGAAGAAAGTTTGCCATATGATATACC
TCACCGAGCACGTATGATCTCTCTAATAGAAAGTGGATGGGCACAAAATC
CAGATGAAAGACCATCTTTCTTAAAATGTTTAATAGAACTTGAACCAGTT
TTGAGAACATTTGAAGAGATAACTTTTCTTGAAGCTGTTATTCAGCTAAA
GAAAACAAAGTTACAGAGTGTTTCAAGTGCCATTCACCTATGTGACAAGA
AGAAAATGGAATTATCTCTGAACATACCTGTAAATCATGGTCCACAAGAG
GAATCATGTGGATCCTCTCAGCTCCATGAAAATAGTGGTTCTCCTGAAAC
TTCAAGGTCCCTGCCAGCTCCTCAAGACAATGATTTTTTATCTAGAAAAG
CTCAAGACTGTTATTTTATGAAGCTGCATCACTGTCCTGGAAATCACAGT
TGGGATAGCACCATTTCTGGATCTCAAAGGGCTGCATTCTGTGATCACAA
GACCACTCCATGCTCTTCAGCAATAATAAATCCACTCTCAACTGCAGGAA
ACTCAGAACGTCTGCAGCCTGGTATAGCCCAGCAGTGGATCCAGAGCAAA
AGGGAAGACATTGTGAACCAAATGACAGAAGCCTGCCTTAACCAGTCGCT
AGATGCCCTTCTGTCCAGGGACTTGATCATGAAAGAGGACTATGAACTTG
TTAGTACCAAGCCTACAAGGACCTCAAAAGTCAGACAATTACTAGACACT
ACTGACATCCAAGGAGAAGAATTTGCCAAAGTTATAGTACAAAAATTGAA
AGATAACAAACAAATGGGTCTTCAGCCTTACCCGGAAATACTTGTGGTTT
CTAGATCACCATCTTTAAATTTACTTCAAAATAAAAGCATGTAAACTAGT

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSRSG
TAAANGEAICSALPTIPYHKLADLRYLSRGASGTVSSARHADWRVQVAVK
HLHIHTPLLDSERKDVLREAEILHKARFSYILPILGICNEPEFLGIVTEY
MPNGSLNELLHRKTEYPDVAWPLRFRILHEIALGVNYLHNMTPPLLHHDL
KTQNILLDNEFHVKIADFGLSKWRMMSLSQSRSSKSAPEGGTIIYMPPEN
YEPGQKSRASIKHDIYSYAVITWEVLSRKQPFEDVTNPLQIMYSVSQGHR
PVINEESLPYDIPHRARMISLIESGWAQNPDERPSFLKCLIELEPVLRTF
EEITFLEAVIQLKKTKLQSVSSAIHLCDKKKMELSLNIPVNHGPQEESCG
SSQLHENSGSPETSRSLPAPQDNDFLSRKAQDCYFMKLHHCPGNHSWDST
ISGSQRAAFCDHKTTPCSSAIINPLSTAGNSERLQPGIAQQWIQSKREDI
VNQMTEACLNQSLDALLSRDLIMKEDYELVSTKPTRTSKVRQLLDTTDIQ
GEEFAKVIVQKLKDNKQMGLQPYPEILVVSRSPSLNLLQNKSM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8790
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express RICK with N terminal GFP/TAPP tag.; N.B. sequence change compared with sequence NP_003812: t1200a (silent in G400). This change present in EST sequences.; ; Estimated MW of expressed protein : 111759 Daltons