cDNA Clone MEKK3

• Synonyms: MAP3K3
• Plasmid: pLent tap MEKK3
• Parent Plasmid: pLent TAP
• Expressed: GFP-TAP-MEKK3
• Tag: GFP
• Tag: TAP
• Species: Human

Sequence of Insert

ACTAGTATGGACGAACAGGAGGCATTGAACTCAATCATGAACGATCTGGT
GGCCCTCCGGATGAACCGACGTCACCGGATGCCTGGATATGAGACCATGA
AGAACAAAGACACAGGTCACTCAAATAGGCAGAGTGACGTCAGAATCAAG
TTCGAGCACAACGGGGAGAGGCGAATTATAGCGTTCAGCCGGCCTGTGAA
ATATGAAGATGTGGAGCACAAGGTGACAACAGTATTTGGACAACCTCTTG
ATCTACATTACATGAACAATGAGCTCTCCATCCTGCTGAAAAACCAAGAT
GATCTTGATAAAGCAATTGACATTTTAGATAGAAGCTCAAGCATGAAAAG
CCTTAGGATATTGCTGTTGTCCCAGGACAGAAACCATAACAGTTCCTCTC
CCCACTCTGGGGTGTCCAGACAGGTGCGGATCAAGGCTTCCCAGTCCGCA
GGGGATATAAATACTATCTACCAGCCCCCCGAGCCCAGAAGCAGGCACCT
CTCTGTCAGCTCCCAGAACCCTGGCCGAAGCTCACCTCCCCCTGGCTATG
TTCCTGAGCGGCAGCAGCACATTGCCCGGCAGGGGTCCTACACCAGCATC
AACAGTGAGGGGGAGTTCATCCCAGAGACCAGCGAGCAGTGCATGCTGGA
TCCCCTGAGCAGTGCAGAAAATTCCTTGTCTGGAAGCTGCCAATCCTTGG
ACAGCCCATCCTTCCGGAAATCACGAATGTCCCGTGCCCAGAGCTTCCCT
GACAACAGACAGGAATACTCAGATCGGGAAACTCAGCTTTATGACAAAGG
GGTCAAAGGTGGAACCTACCCCCGGCGCTACCACGTGTCTGTGCACCACA
AGGACTACAGTGATGGCAGAAGAACATTTCCCCGAATACGGCGTCATCAA
GGCAACTTGTTCACCCTGGTGCCCTCCAGCCGCTCCCTGAGCACAAATGG
CGAGAACATGGGCCTGGCTGTGCAATACCTGGACCCCCGTGGGCGCCTGC
GGAGTGCGGACAGCGAGAATGCCCTCTCTGTGCAGGAGAGGAATGTGCCA
ACCAAGTCTCCCAGTGCCCCCATCAACTGGCGCCGGGGAAAGCTCCTGGG
CCAGGGTGCCTTCGGCAGGGTCTATTTGTGCTATGACGTGGACACGGGAC
GTGAACTTGCTTCCAAGCAGGTCCAATTTGATCCAGACAGTCCTGAGACA
AGCAAGGAGGTGAGTGCTCTGGAGTGCGAGATCCAGTTGCTAAAGAACTT
GCAGCATGAGCGCATCGTGCAGTACTATGGCTGTCTGCGGGACCGCGCTG
AGAAGACCCTGACCATCTTCATGGAGTACATGCCAGGGGGCTCGGTGAAA
GACCAGTTGAAGGCTTACGGTGCTCTGACAGAGAGCGTGACCCGAAAGTA
CACGCGGCAGATCCTGGAGGGCATGTCCTACCTGCACAGCAACATGATTG
TTCACCGGGACATTAAGGGAGCCAACATCCTCCGAGACTCTGCTGGGAAT
GTAAAGCTGGGGGACTTTGGGGCCAGCAAACGCCTGCAGACGATCTGTAT
GTCGGGGACGGGCATGCGCTCCGTCACTGGCACACCCTACTGGATGAGCC
CTGAGGTGATCAGCGGCGAGGGCTATGGAAGGAAAGCAGACGTGTGGAGC
CTGGGCTGCACTGTGGTGGAGATGCTGACAGAGAAACCACCGTGGGCAGA
GTATGAAGCTATGGCCGCCATCTTCAAGATTGCCACCCAGCCCACCAATC
CTCAGCTGCCCTCCCACATCTCTGAACATGGCCGGGACTTCCTGAGGCGC
ATTTTTGTGGAGGCTCGCCAGAGACCTTCAGCTGAGGAGCTGCTCACACA
CCACTTTGCACAGCTCATGTACTGAACTAGT

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLTSMDE
QEALNSIMNDLVALRMNRRHRMPGYETMKNKDTGHSNRQSDVRIKFEHNG
ERRIIAFSRPVKYEDVEHKVTTVFGQPLDLHYMNNELSILLKNQDDLDKA
IDILDRSSSMKSLRILLLSQDRNHNSSSPHSGVSRQVRIKASQSAGDINT
IYQPPEPRSRHLSVSSQNPGRSSPPPGYVPERQQHIARQGSYTSINSEGE
FIPETSEQCMLDPLSSAENSLSGSCQSLDSPSFRKSRMSRAQSFPDNRQE
YSDRETQLYDKGVKGGTYPRRYHVSVHHKDYSDGRRTFPRIRRHQGNLFT
LVPSSRSLSTNGENMGLAVQYLDPRGRLRSADSENALSVQERNVPTKSPS
APINWRRGKLLGQGAFGRVYLCYDVDTGRELASKQVQFDPDSPETSKEVS
ALECEIQLLKNLQHERIVQYYGCLRDRAEKTLTIFMEYMPGGSVKDQLKA
YGALTESVTRKYTRQILEGMSYLHSNMIVHRDIKGANILRDSAGNVKLGD
FGASKRLQTICMSGTGMRSVTGTPYWMSPEVISGEGYGRKADVWSLGCTV
VEMLTEKPPWAEYEAMAAIFKIATQPTNPQLPSHISEHGRDFLRRIFVEA
RQRPSAEELLTHHFAQLMY*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8789
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GFP-TAP for mammalian expression.; ; Estimated MW of expressed protein : 120621 Daltons