cDNA Clone DDB1

• Plasmid: pGEX6P-2-DDB1
• Parent Plasmid: pGEX6P2
• Expressed: GST-DDB1
• Genbank: NP_001914.2
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGTCGTACAACTACGTGGTAACGGCCCAGAAGCCCACCGC
CGTGAACGGCTGCGTGACCGGACACTTTACTTCGGCCGAAGACTTAAACC
TGTTGATTGCCAAAAACACGAGATTAGAGATCTATGTGGTCACCGCCGAG
GGGCTTCGGCCCGTCAAAGAGGTGGGCATGTATGGGAAGATTGCGGTCAT
GGAGCTTTTCAGGCCCAAGGGGGAGAGCAAGGACCTGCTGTTTATCTTGA
CAGCGAAGTACAATGCCTGCATCCTGGAGTATAAACAGAGTGGCGAGAGC
ATTGACATCATTACGCGAGCCCATGGCAATGTCCAGGACCGCATTGGCCG
CCCCTCAGAGACCGGCATTATTGGCATCATTGACCCTGAGTGCCGGATGA
TTGGCCTGCGTCTCTATGATGGCCTTTTCAAGGTTATTCCACTAGATCGC
GATAATAAAGAACTCAAGGCCTTCAACATCCGCCTGGAGGAGCTGCATGT
CATTGATGTCAAGTTCCTATATGGTTGCCAAGCACCTACTATTTGCTTTG
TCTACCAGGACCCTCAGGGGCGGCACGTAAAAACCTATGAGGTGTCTCTC
CGAGAAAAGGAATTCAATAAGGGCCCTTGGAAACAGGAAAATGTCGAAGC
TGAAGCTTCCATGGTGATCGCAGTCCCAGAGCCCTTTGGGGGGGCCATCA
TCATTGGACAGGAGTCAATCACCTATCACAATGGTGACAAATACCTGGCT
ATTGCCCCTCCTATCATCAAGCAAAGCACGATTGTGTGCCACAATCGAGT
GGACCCTAATGGCTCAAGATACCTGCTGGGAGACATGGAAGGCCGGCTCT
TCATGCTGCTTTTGGAGAAGGAGGAACAGATGGATGGCACCGTCACTCTC
AAGGATCTCCGTGTAGAACTCCTTGGAGAGACCTCTATTGCTGAGTGCTT
GACATACCTTGATAATGGTGTTGTGTTTGTCGGGTCTCGCCTGGGTGACT
CCCAGCTTGTGAAGCTCAACGTTGACAGTAATGAACAAGGCTCCTATGTA
GTGGCCATGGAAACCTTTACCAACTTAGGACCCATTGTCGATATGTGCGT
GGTGGACCTGGAGAGGCAGGGGCAGGGGCAGCTGGTCACTTGCTCTGGGG
CTTTCAAGGAAGGTTCTTTGCGGATCATCCGGAATGGAATTGGAATCCAC
GAGCATGCCAGCATTGACTTACCAGGCATCAAAGGATTATGGCCACTGCG
GTCTGACCCTAATCGTGAGACTTATGACACTTTGGTGCTCTCTTTTGTGG
GCCAGACAAGAGTTCTCATGTTAAATGGAGAGGAGGTAGAAGAAACCGAA
CTGATGGGTTTCGTGGATGATCAGCAGACTTTCTTCTGTGGCAACGTGGC
TCATCAGCAGCTTATCCAGATCACTTCAGCATCGGTGAGGTTGGTCTCTC
AAGAACCCAAAGCTCTGGTCAGTGAATGGAAGGAGCCTCAGGCCAAGAAC
ATCAGTGTGGCCTCCTGCAATAGCAGCCAGGTGGTGGTGGCTGTAGGCAG
GGCCCTCTACTATCTGCAGATCCATCCTCAGGAGCTCCGGCAGATCAGCC
ACACAGAGATGGAACATGAAGTGGCTTGCTTGGACATCACCCCATTAGGA
GACAGCAATGGACTGTCCCCTCTTTGTGCCATTGGCCTCTGGACGGACAT
CTCGGCTCGTATCTTGAAGTTGCCCTCTTTTGAACTACTGCACAAGGAGA
TGCTGGGTGGAGAGATCATTCCTCGCTCCATCCTGATGACCACCTTTGAG
AGTAGCCATTACCTCCTTTGTGCCTTGGGAGATGGAGCGCTTTTCTACTT
TGGGCTCAACATTGAGACAGGTCTGTTGAGCGACCGTAAGAAGGTGACTT
TGGGCACCCAGCCCACCGTATTGAGGACTTTTCGTTCTCTTTCTACCACC
AACGTCTTTGCTTGTTCTGACCGCCCCACTGTCATCTATAGCAGCAACCA
CAAATTGGTCTTCTCAAATGTCAACCTCAAGGAAGTGAACTACATGTGTC
CCCTCAATTCAGATGGCTATCCTGACAGCCTGGCGCTGGCCAACAATAGC
ACCCTCACCATTGGCACCATCGATGAGATCCAGAAGCTGCACATTCGCAC
AGTTCCCCTCTATGAGTCTCCAAGGAAGATCTGCTACCAGGAAGTGTCCC
AGTGTTTCGGGGTCCTCTCCAGCCGCATTGAAGTCCAAGACACGAGTGGG
GGCACGACAGCCTTGAGGCCCAGCGCTAGCACCCAGGCTCTGTCCAGCAG
TGTAAGCTCCAGCAAGCTGTTCTCCAGCAGCACTGCTCCTCATGAGACCT
CCTTTGGAGAAGAGGTGGAGGTGCATAACCTACTTATCATTGACCAACAC
ACCTTTGAAGTGCTTCATGCCCACCAGTTTCTGCAGAATGAATATGCCCT
CAGTCTGGTTTCCTGCAAGCTGGGCAAAGACCCCAACACTTACTTCATTG
TGGGCACAGCAATGGTGTATCCTGAAGAGGCAGAGCCCAAGCAGGGTCGC
ATTGTGGTCTTTCAGTATTCGGATGGAAAACTACAGACTGTGGCTGAAAA
GGAAGTGAAAGGGGCCGTGTACTCTATGGTGGAATTTAACGGGAAGCTGT
TAGCCAGCATCAATAGCACGGTGCGGCTCTATGAGTGGACAACAGAGAAG
GACGTGCGCACTGAGTGCAACCACTACAACAACATCATGGCCCTCTACCT
GAAGACCAAGGGCGACTTCATCCTGGTGGGCGACCTTATGCGCTCAGTGC
TGCTGCTTGCCTACAAGCCCATGGAAGGAAACTTTGAAGAGATTGCTCGA
GACTTTAATCCCAACTGGATGAGTGCTGTGGAAATCTTGGATGATGACAA
TTTTCTGGGGGCTGAAAATGCCTTTAACTTGTTTGTGTGTCAAAAGGATA
GCGCTGCCACCACTGACGAGGAGCGGCAGCACCTCCAGGAGGTTGGTCTT
TTCCACCTGGGCGAGTTTGTCAATGTCTTTTGCCACGGCTCTCTGGTAAT
GCAGAATCTGGGTGAGACTTCCACCCCCACACAAGGCTCGGTGCTCTTCG
GCACGGTCAACGGCATGATAGGGCTGGTGACCTCACTGTCAGAGAGCTGG
TACAACCTCCTGCTGGACATGCAGAATCGACTCAATAAAGTCATCAAAAG
TGTGGGGAAGATCGAGCACTCCTTCTGGAGATCCTTTCACACCGAGCGGA
AGACAGAACCAGCCACAGGTTTCATCGACGGTGACTTGATTGAGAGTTTC
CTGGATATTAGCCGCCCCAAGATGCAGGAGGTGGTGGCAAACCTACAGTA
TGACGATGGCAGCGGTATGAAGCGAGAGGCCACTGCAGACGACCTCATCA
AGGTTGTGGAGGAGCTAACTCGGATCCATTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMSYNYVVT
AQKPTAVNGCVTGHFTSAEDLNLLIAKNTRLEIYVVTAEGLRPVKEVGMY
GKIAVMELFRPKGESKDLLFILTAKYNACILEYKQSGESIDIITRAHGNV
QDRIGRPSETGIIGIIDPECRMIGLRLYDGLFKVIPLDRDNKELKAFNIR
LEELHVIDVKFLYGCQAPTICFVYQDPQGRHVKTYEVSLREKEFNKGPWK
QENVEAEASMVIAVPEPFGGAIIIGQESITYHNGDKYLAIAPPIIKQSTI
VCHNRVDPNGSRYLLGDMEGRLFMLLLEKEEQMDGTVTLKDLRVELLGET
SIAECLTYLDNGVVFVGSRLGDSQLVKLNVDSNEQGSYVVAMETFTNLGP
IVDMCVVDLERQGQGQLVTCSGAFKEGSLRIIRNGIGIHEHASIDLPGIK
GLWPLRSDPNRETYDTLVLSFVGQTRVLMLNGEEVEETELMGFVDDQQTF
FCGNVAHQQLIQITSASVRLVSQEPKALVSEWKEPQAKNISVASCNSSQV
VVAVGRALYYLQIHPQELRQISHTEMEHEVACLDITPLGDSNGLSPLCAI
GLWTDISARILKLPSFELLHKEMLGGEIIPRSILMTTFESSHYLLCALGD
GALFYFGLNIETGLLSDRKKVTLGTQPTVLRTFRSLSTTNVFACSDRPTV
IYSSNHKLVFSNVNLKEVNYMCPLNSDGYPDSLALANNSTLTIGTIDEIQ
KLHIRTVPLYESPRKICYQEVSQCFGVLSSRIEVQDTSGGTTALRPSAST
QALSSSVSSSKLFSSSTAPHETSFGEEVEVHNLLIIDQHTFEVLHAHQFL
QNEYALSLVSCKLGKDPNTYFIVGTAMVYPEEAEPKQGRIVVFQYSDGKL
QTVAEKEVKGAVYSMVEFNGKLLASINSTVRLYEWTTEKDVRTECNHYNN
IMALYLKTKGDFILVGDLMRSVLLLAYKPMEGNFEEIARDFNPNWMSAVE
ILDDDNFLGAENAFNLFVCQKDSAATTDEERQHLQEVGLFHLGEFVNVFC
HGSLVMQNLGETSTPTQGSVLFGTVNGMIGLVTSLSESWYNLLLDMQNRL
NKVIKSVGKIEHSFWRSFHTERKTEPATGFIDGDLIESFLDISRPKMQEV
VANLQYDDGSGMKREATADDLIKVVEELTRIH

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8753
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GSt for bacterial expression.; ; Estimated MW of expressed protein : 154787 Daltons