cDNA Clone Ubiquitin

• Plasmid: pET15b6P Ubiquitin K33R
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His Ubiquitin K33R
• Genbank: Pet15-6P
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGA
AGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACA
GGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGACTCGAG

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSQIFVKTLTGKTITLEVEPSDTIENVKA
KIQDREGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8725
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His for bacterial expression.