cDNA Clone DDI2

• Plasmid: pGEX6P-1-DDI2
• Parent Plasmid: pGEX6P1
• Expressed: GST-DDI2
• Genbank: NP_115717.3
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCTGCTCACCGTGTACTGTGTGCGGAGGGACCTCTCCGAGGT
GACCTTTTCCCTCCAGGTCGACGCCGACTTCGAGCTGCACAACTTCCGCG
CGCTGTGCGAGCTCGAGTCTGGCATCCCCGCAGCCGAGAGCCAGATCGTC
TATGCGGAAAGACCTCTCACAGACAACCACAGATCATTGGCTTCTTATGG
CTTGAAAGATGGGGACGTTGTGATTTTACGACAGAAGGAGAATGCAGACC
CTCGACCTCCAGTGCAGTTCCCAAACTTACCCCGAATAGATTTCAGTAGT
ATAGCTGTGCCTGGCACATCAAGTCCCCGGCAGCGCCAGCCACCAGGAAC
ACAGCAGTCCCACTCATCTCCTGGAGAAATAACTTCATCTCCTCAGGGCT
TGGACAATCCAGCCTTGCTCCGAGATATGTTGCTGGCCAACCCGCATGAG
CTGTCCTTGCTGAAGGAACGCAATCCACCCCTGGCAGAAGCTCTGCTCAG
TGGAGACCTTGAGAAATTTTCTAGAGTCCTGGTGGAGCAGCAGCAGGACC
GAGCCCGGAGAGAGCAAGAAAGGATTCGTCTGTTTTCTGCTGATCCCTTT
GACCTTGAAGCTCAGGCAAAGATAGAAGAAGATATAAGGCAACAGAACAT
TGAGGAAAACATGACAATAGCTATGGAAGAGGCTCCGGAAAGTTTTGGCC
AAGTAGTGATGCTTTATATTAACTGCAAAGTGAATGGACATCCTGTGAAA
GCCTTTGTTGACTCAGGTGCCCAGATGACTATCATGAGCCAAGCTTGTGC
AGAAAGGTGTAACATAATGAGACTGGTGGACCGTCGGTGGGCAGGGATTG
CCAAAGGAGTGGGCACCCAGAAGATTATTGGAAGGGTACATCTAGCTCAG
GTTCAGATTGAAGGAGATTTTTTGCCATGTTCCTTCTCTATACTTGAGGA
ACAGCCCATGGACATGCTTCTGGGACTGGACATGCTTAAACGGCACCAGT
GTTCCATCGACCTGAAGAAAAATGTACTCGTGATCGGCACCACAGGCTCC
CAGACCACCTTTCTTCCTGAGGGAGAGCTACCAGAGTGTGCCCGGTTGGC
ATATGGGGCTGGAAGAGAGGATGTACGGCCAGAGGAGATTGCAGACCAAG
AATTAGCAGAAGCCCTTCAAAAATCAGCAGAGGATGCAGAGCGTCAGAAG
CCATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMLLTVYCVRRDLSEVTFSL
QVDADFELHNFRALCELESGIPAAESQIVYAERPLTDNHRSLASYGLKDG
DVVILRQKENADPRPPVQFPNLPRIDFSSIAVPGTSSPRQRQPPGTQQSH
SSPGEITSSPQGLDNPALLRDMLLANPHELSLLKERNPPLAEALLSGDLE
KFSRVLVEQQQDRARREQERIRLFSADPFDLEAQAKIEEDIRQQNIEENM
TIAMEEAPESFGQVVMLYINCKVNGHPVKAFVDSGAQMTIMSQACAERCN
IMRLVDRRWAGIAKGVGTQKIIGRVHLAQVQIEGDFLPCSFSILEEQPMD
MLLGLDMLKRHQCSIDLKKNVLVIGTTGSQTTFLPEGELPECARLAYGAG
REDVRPEEIADQELAEALQKSAEDAERQKP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8665
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 71344 Daltons