cDNA Clone TNF alpha

• Plasmid: pEBG6P-TNFalpha 77-234
• Parent Plasmid: pEBG6P
• Expressed: GST-TNFalpha 77-234
• Genbank: AAH28148.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGTCAGATCATCTTCTCGAACCCCGAGTGACAAGCCTGTAGCCCA
TGTTGTAGCAAACCCTCAAGCTGAGGGGCAGCTCCAGTGGCTGAACCGCC
GGGCCAATGCCCTCCTGGCCAATGGCGTGGAGCTGAGAGATAACCAGCTG
GTGGTGCCATCAGAGGGCCTGTACCTCATCTACTCCCAGGTCCTCTTCAA
GGGCCAAGGCTGCCCCTCCACCCATGTGCTCCTCACCCACACCATCAGCC
GCATCGCCGTCTCCTACCAGACCAAGGTCAACCTCCTCTCTGCCATCAAG
AGCCCCTGCCAGAGGGAGACCCCAGAGGGGGCTGAGGCCAAGCCCTGGTA
TGAGCCCATCTATCTGGGAGGGGTCTTCCAGCTGGAGAAGGGTGACCGAC
TCAGCGCTGAGATCAATCGGCCCGACTATCTCGACTTTGCCGAGTCTGGG
CAGGTCTACTTTGGGATCATTGCCCTGTGAGGATCC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSVRSSSRTPSDKPV
AHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVL
FKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKP
WYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8655
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; Estimated MW of expressed protein : 44769 Daltons