cDNA Clone RIPK1

• Plasmid: pCMV-FLAG-2 RIPK1
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG-RIPK1
• Genbank: NM_003804
• Tag: FLAG
• Species: Human

Sequence of Insert

GCGGCCGCGCAACCAGACATGTCCTTGAATGTCATTAAGATGAAATCCAG
TGACTTCCTGGAGAGTGCAGAACTGGACAGCGGAGGCTTCGGGAAGGTGT
CTCTGTGTTTCCACAGAACCCAGGGACTCATGATCATGAAAACAGTGTAC
AAGGGGCCCAACTGCATTGAGCACAACGAGGCCCTCTTGGAGGAGGCGAA
GATGATGAACAGACTGAGACACAGCCGGGTGGTGAAGCTCCTGGGCGTCA
TCATAGAGGAAGGGAAGTACTCCCTGGTGATGGAGTACATGGAGAAGGGC
AACCTGATGCACGTGCTGAAAGCCGAGATGAGTACTCCGCTTTCTGTAAA
AGGAAGGATAATTTTGGAAATCATTGAAGGAATGTGCTACTTACATGGAA
AAGGCGTGATACACAAGGACCTGAAGCCTGAAAATATCCTTGTTGATAAT
GACTTCCACATTAAGATCGCAGACCTCGGCCTTGCCTCCTTTAAGATGTG
GAGCAAACTGAATAATGAAGAGCACAATGAGCTGAGGGAAGTGGACGGCA
CCGCTAAGAAGAATGGCGGCACCCTCTACTACATGGCGCCCGAGCACCTG
AATGACGTCAACGCAAAGCCCACAGAGAAGTCGGATGTGTACAGCTTTGC
TGTAGTACTCTGGGCGATATTTGCAAATAAGGAGCCATATGAAAATGCTA
TCTGTGAGCAGCAGTTGATAATGTGCATAAAATCTGGGAACAGGCCAGAT
GTGGATGACATCACTGAGTACTGCCCAAGAGAAATTATCAGTCTCATGAA
GCTCTGCTGGGAAGCGAATCCGGAAGCTCGGCCGACATTTCCTGGCATTG
AAGAAAAATTTAGGCCTTTTTATTTAAGTCAATTAGAAGAAAGTGTAGAA
GAGGACGTGAAGAGTTTAAAGAAAGAGTATTCAAACGAAAATGCAGTTGT
GAAGAGAATGCAGTCTCTTCAACTTGATTGTGTGGCAGTACCTTCAAGCC
GGTCAAATTCAGCCACAGAACAGCCTGGTTCACTGCACAGTTCCCAGGGA
CTTGGGATGGGTCCTGTGGAGGAGTCCTGGTTTGCTCCTTCCCTGGAGCA
CCCACAAGAAGAGAATGAGCCCAGCCTGCAGAGTAAACTCCAAGACGAAG
CCAACTACCATCTTTATGGCAGCCGCATGGACAGGCAGACGAAACAGCAG
CCCAGACAGAATGTGGCTTACAACAGAGAGGAGGAAAGGAGACGCAGGGT
CTCCCATGACCCTTTTGCACAGCAAAGACCTTACGAGAATTTTCAGAATA
CAGAGGGAAAAGGCACTGCTTATTCCAGTGCAGCCAGTCATGGTAATGCA
GTGCACCAGCCCTCAGGGCTCACCAGCCAACCTCAAGTACTGTATCAGAA
CAATGGATTATATAGCTCACATGGCTTTGGAACAAGACCACTGGATCCAG
GAACAGCAGGTCCCAGAGTTTGGTACAGGCCAATTCCAAGTCATATGCCT
AGTCTGCATAATATCCCAGTGCCTGAGACCAACTATCTAGGAAATACACC
CACCATGCCATTCAGCTCCTTGCCACCAACAGATGAATCTATAAAATATA
CCATATACAATAGTACTGGCATTCAGATTGGAGCCTACAATTATATGGAG
ATTGGTGGGACGAGTTCATCACTACTAGACAGCACAAATACGAACTTCAA
AGAAGAGCCAGCTGCTAAGTACCAAGCTATCTTTGATAATACCACTAGTC
TGACGGATAAACACCTGGACCCAATCAGGGAAAATCTGGGAAAGCACTGG
AAAAACTGTGCCCGTAAACTGGGCTTCACACAGTCTCAGATTGATGAAAT
TGACCATGACTATGAGCGAGATGGACTGAAAGAAAAGGTTTACCAGATGC
TCCAAAAGTGGGTGATGAGGGAAGGCATAAAGGGAGCCACGGTGGGGAAG
CTGGCCCAGGCGCTCCACCAGTGTTCCAGGATCGACCTTCTGAGCAGCTT
GATTTACGTCAGCCAGAACTAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGIPGIPGSTRAAAQPDMSLNVIKMKSSDFLESAELDSGGFG
KVSLCFHRTQGLMIMKTVYKGPNCIEHNEALLEEAKMMNRLRHSRVVKLL
GVIIEEGKYSLVMEYMEKGNLMHVLKAEMSTPLSVKGRIILEIIEGMCYL
HGKGVIHKDLKPENILVDNDFHIKIADLGLASFKMWSKLNNEEHNELREV
DGTAKKNGGTLYYMAPEHLNDVNAKPTEKSDVYSFAVVLWAIFANKEPYE
NAICEQQLIMCIKSGNRPDVDDITEYCPREIISLMKLCWEANPEARPTFP
GIEEKFRPFYLSQLEESVEEDVKSLKKEYSNENAVVKRMQSLQLDCVAVP
SSRSNSATEQPGSLHSSQGLGMGPVEESWFAPSLEHPQEENEPSLQSKLQ
DEANYHLYGSRMDRQTKQQPRQNVAYNREEERRRRVSHDPFAQQRPYENF
QNTEGKGTAYSSAASHGNAVHQPSGLTSQPQVLYQNNGLYSSHGFGTRPL
DPGTAGPRVWYRPIPSHMPSLHNIPVPETNYLGNTPTMPFSSLPPTDESI
KYTIYNSTGIQIGAYNYMEIGGTSSSLLDSTNTNFKEEPAAKYQAIFDNT
TSLTDKHLDPIRENLGKHWKNCARKLGFTQSQIDEIDHDYERDGLKEKVY
QMLQKWVMREGIKGATVGKLAQALHQCSRIDLLSSLIYVSQN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8614
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter FLAg for mammalian expression.; N.B. Sequence differences compared with NM_003804 CDS:; t84c (silent in F28); t1313c (V438A). ; Both present in other published mRNAs for RIPK1.; ; Estimated MW of expressed protein : 78073 Daltons