cDNA Clone Ubiquitin

• Plasmid: pGFP TAPP Ubiquitin K63R
• Parent Plasmid: pGFP TAP BN
• Expressed: GFP-TAP-Ubiquitin K63R
• Genbank: P62988
• Tag: GFP
• Tag: TAP
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCCAGATCTT
CGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTG
ACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCT
CCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACG
CACCCTGTCTGACTACAACATCCAGAGAGAGTCCACCCTGCACCTGGTCC
TCCGTCTCAGAGGTGGGTGATAAGGATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSATM
YPYDVPDYAQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQR
LIFAGKQLEDGRTLSDYNIQRESTLHLVLRLRGG**

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8215
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GFP/TAPP/HA tag for mammalian expression.; Estimated MW of expressed protein : 59932 Daltons