cDNA Clone CAMKK beta

• Synonyms: CAMKK2
• Plasmid: pGEX6P-1-CAMKK beta *dont use*
• Parent Plasmid: pGEX6P1
• Genbank: NM_153499
• Species: Human

Sequence of Insert

GGATCCTCATCATGTGTCTCTAGCCAGCCCAGCAGCAACCGGGCCGCCCC
CCAGGATGAGCTGGGGGGCAGGGGCAGCAGCAGCAGCGAAAGCCAGAAGC
CCTGTGAGGCCCTGCGGGGCCTCTCATCCTTGAGCATCCACCTGGGCATG
GAGTCCTTCATTGTGGTCACCGAGTGTGAGCCGGGCTGTGCTGTGGACCT
CGGCTTGGCGCGGGACCGGCCCCTGGAGGCCGATGGCCAAGAGGTCCCCC
TTGACACCTCCGGGTCCCAGGCCCGGCCCCACCTCTCCGGTCGCAAGCTG
TCTCTGCAAGAGCGGTCCCAGGGTGGGCTGGCAGCCGGTGGCAGCCTGGA
CATGAACGGACGCTGCATCTGCCCGTCCCTGCCCTACTCACCCGTCAGCT
CCCCGCAGTCCTCGCCTCGGCTGCCCCGGCGGCCGACAGTGGAGTCTCAC
CACGTCTCCATCACGGGTATGCAGGACTGTGTGCAGCTGAATCAGTATAC
CCTGAAGGATGAAATTGGAAAGGGCTCCTATGGTGTCGTCAAGTTGGCCT
ACAATGAAAATGACAATACCTACTATGCAATGAAGGTGCTGTCCAAAAAG
AAGCTGATCCGGCAGGCCGGCTTTCCACGTCGCCCTCCACCCCGAGGCAC
CCGGCCAGCTCCTGGAGGCTGCATCCAGCCCAGGGGCCCCATTGAGCAGG
TGTACCAGGAAATTGCCATCCTCAAGAAGCTGGACCACCCCAATGTGGTG
AAGCTGGTGGAGGTCCTGGATGACCCCAATGAGGACCATCTGTACATGGT
GTTCGAACTGGTCAACCAAGGGCCCGTGATGGAAGTGCCCACCCTCAAAC
CACTCTCTGAAGACCAGGCCCGTTTCTACTTCCAGGATCTGATCAAAGGC
ATCGAGTACTTACACTACCAGAAGATCATCCACCGTGACATCAAACCTTC
CAACCTCCTGGTCGGAGAAGATGGGCACATCAAGATCGCTGACTTTGGTG
TGAGCAATGAATTCAAGGGCAGTGACGCGCTCCTCTCCAACACCGTGGGC
ACGCCCGCCTTCATGGCACCCGAGTCGCTCTCTGAGACCCGCAAGATATT
CTCTGGGAAGGCCTTGGATGTTTGGGCCATGGGTGTGACACTATACTGCT
TTGTCTTTGGCCAGTGCCCATTCATGGACGAGCGGATCATGTGTTTACAC
AGTAAGATCAAGAGTCAGGCCCTGGAATTTCCAGACCAGCCCGACATAGC
TGAGGACTTGAAGGACCTGATCACCCGTATGCTGGACAAGAACCCCGAGT
CGAGGATCGTGGTGCCGGAAATCAAGCTGCACCCCTGGGTCACGAGGCAT
GGGGCGGAGCCGTTGCCGTCGGAGGATGAGAACTGCACGCTGGTCGAAGT
GACTGAAGAGGAGGTCGAGAACTCAGTCAAACACATTCCCAGCTTGGCAA
CCGTGATCCTGGTGAAGACCATGATACGTAAACGCTCCTTTGGGAACCCA
TTCGAGGGCAGCCGGCGGGAGGAACGCTCACTGTCAGCGCCTGGAAACTT
GCTCACCAAAAAACCAACCAGGGAATGTGAGTCCCTGTCTGAGCTCAAGG
AAGCAAGGCAGCGAAGACAACCTCCAGGGCACCGACCCGCCCCCCGTGGG
GGAGGAGGAAGTGCTCTTGCACCACCACCACCACCACCACCTCGAGCGGC
CGCATCGTGACTGA

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSSSCVSSQPSSNRAAPQDEL
GGRGSSSSESQKPCEALRGLSSLSIHLGMESFIVVTECEPGCAVDLGLAR
DRPLEADGQEVPLDTSGSQARPHLSGRKLSLQERSQGGLAAGGSLDMNGR
CICPSLPYSPVSSPQSSPRLPRRPTVESHHVSITGMQDCVQLNQYTLKDE
IGKGSYGVVKLAYNENDNTYYAMKVLSKKKLIRQAGFPRRPPPRGTRPAP
GGCIQPRGPIEQVYQEIAILKKLDHPNVVKLVEVLDDPNEDHLYMVFELV
NQGPVMEVPTLKPLSEDQARFYFQDLIKGIEYLHYQKIIHRDIKPSNLLV
GEDGHIKIADFGVSNEFKGSDALLSNTVGTPAFMAPESLSETRKIFSGKA
LDVWAMGVTLYCFVFGQCPFMDERIMCLHSKIKSQALEFPDQPDIAEDLK
DLITRMLDKNPESRIVVPEIKLHPWVTRHGAEPLPSEDENCTLVEVTEEE
VENSVKHIPSLATVILVKTMIRKRSFGNPFEGSRREERSLSAPGNLLTKK
PTRECESLSELKEARQRRQPPGHRPAPRGGGGSALAPPPPPPPRAAAS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8205
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression. Expressed protein contains an C terminal tail compared to genbank. (PTRECESLSELKEARQRRQPPGHRPAPRGGGGSALAPPPPPPPRAAAS); ; Estimated MW of expressed protein : 89086 Daltons