cDNA Clone CHD1 (yeast)

• Synonyms: YER164W
• Plasmid: pGEX6P1 CHD1 yeast
• Parent Plasmid: pGEX6P1
• Expressed: GST 3C CHD1 yeast
• Genbank: NM_001179054.1
• Tag: GST
• Species: Saccharomyces cerevisiae

Sequence of Insert

GGATCCATGGCAGCCAAGGATATTTCTACTGAAGTGCTGCAAAATCCTGA
ACTATATGGGCTGAGGCGGTCACATAGAGCTGCTGCGCATCAACAAAACT
ACTTCAACGATAGCGATGATGAGGACGATGAAGATAATATTAAACAGTCG
AGGAGGAAGAGAATGACCACGATTGAAGACGACGAAGACGAATTTGAAGA
TGAAGAAGGCGAAGAAGATTCTGGAGAGGACGAAGATGAGGAAGATTTTG
AAGAAGATGATGATTATTATGGCTCTCCTATAAAACAAAATCGATCAAAA
CCGAAATCAAGAACAAAATCAAAGTCTAAATCTAAGCCAAAATCTCAATC
TGAAAAGCAATCTACAGTGAAAATACCAACAAGGTTTTCTAATCGTCAAA
ACAAAACGGTAAATTATAACATCGATTATAGCGATGATGACCTCTTAGAA
TCTGAAGATGACTATGGTTCCGAGGAGGCTTTATCAGAGGAAAATGTGCA
TGAAGCATCTGCCAATCCTCAACCAGAGGACTTCCACGGTATTGATATTG
TTATCAATCACAGACTAAAGACATCTTTGGAAGAAGGAAAGGTCTTGGAA
AAGACTGTTCCTGACCTCAATAACTGCAAGGAAAATTATGAATTCTTAAT
CAAATGGACAGATGAATCTCATCTCCACAATACCTGGGAAACTTATGAAT
CCATTGGCCAAGTCCGTGGATTGAAGAGGTTGGACAACTATTGTAAGCAA
TTCATCATCGAAGATCAACAAGTAAGATTAGATCCATACGTTACCGCCGA
AGATATAGAGATCATGGACATGGAACGTGAACGTAGGCTTGATGAATTTG
AGGAGTTCCACGTGCCAGAAAGGATTATTGATAGCCAACGTGCTTCGTTG
GAAGACGGCACCTCACAATTACAGTATTTAGTTAAATGGCGTCGTCTAAA
TTACGACGAGGCTACTTGGGAGAATGCCACAGATATCGTGAAATTGGCAC
CTGAACAAGTGAAACATTTTCAAAACAGAGAAAACTCTAAGATCCTCCCA
CAATATTCCAGTAATTATACTTCACAGAGACCGCGTTTTGAGAAGTTAAG
CGTGCAACCTCCGTTCATTAAAGGTGGGGAATTAAGAGATTTTCAACTAA
CTGGTATTAATTGGATGGCATTTTTGTGGTCCAAAGGTGATAATGGTATA
CTGGCAGATGAGATGGGCCTGGGAAAAACGGTCCAGACTGTCGCCTTTAT
CAGTTGGCTGATATTTGCTCGTAGACAAAACGGACCTCACATCATTGTCG
TTCCTTTATCGACAATGCCTGCCTGGTTGGATACTTTTGAGAAATGGGCG
CCTGATTTGAATTGTATATGCTATATGGGCAACCAAAAATCAAGAGATAC
CATTCGAGAATATGAATTTTACACCAATCCAAGGGCAAAAGGGAAAAAAA
CAATGAAATTTAATGTTTTATTAACAACATACGAGTACATCTTAAAGGAT
CGTGCTGAATTAGGAAGTATAAAATGGCAATTTATGGCCGTTGACGAAGC
TCATAGACTAAAAAATGCTGAATCATCCCTTTATGAATCATTAAACAGTT
TCAAGGTCGCCAACCGTATGTTAATCACAGGCACACCTCTTCAGAATAAT
ATTAAAGAGTTAGCTGCGTTGGTTAATTTCCTAATGCCCGGAAGGTTTAC
GATTGATCAGGAGATTGATTTTGAAAACCAAGATGAAGAGCAAGAAGAAT
ATATTCATGATTTACACCGAAGAATACAGCCTTTTATTCTTCGTCGGTTG
AAGAAAGACGTAGAAAAATCACTTCCATCAAAGACAGAGCGTATTTTAAG
AGTTGAATTGTCCGACGTACAGACTGAGTACTATAAAAATATTCTGACTA
AAAACTACTCTGCTTTAACTGCTGGAGCTAAAGGGGGTCATTTCTCTTTA
CTGAATATTATGAACGAGTTGAAAAAGGCATCGAACCATCCATATCTCTT
CGATAATGCTGAAGAGCGCGTCTTACAGAAATTTGGGGATGGTAAAATGA
CTCGAGAAAACGTACTAAGAGGTTTGATCATGTCTTCGGGTAAGATGGTT
CTTTTAGACCAATTATTGACCAGATTGAAGAAAGATGGGCACCGCGTGTT
GATTTTTTCACAAATGGTCAGAATGCTTGACATTTTAGGCGACTATTTAT
CCATTAAAGGTATTAACTTCCAAAGGTTAGATGGTACAGTACCATCTGCT
CAAAGAAGGATATCTATTGATCATTTCAACTCTCCGGATTCAAACGACTT
CGTATTTTTACTTTCTACTCGTGCTGGTGGTTTGGGTATCAACTTAATGA
CCGCCGATACCGTTGTGATTTTTGATTCCGATTGGAATCCGCAAGCCGAT
TTACAAGCAATGGCTAGAGCTCATCGTATTGGCCAAAAAAATCACGTTAT
GGTGTATAGGTTGGTTTCAAAAGACACAGTAGAGGAAGAGGTATTAGAAA
GAGCACGGAAGAAAATGATTCTGGAATATGCTATTATTTCTCTTGGAGTG
ACAGATGGTAACAAATACACTAAGAAGAATGAACCAAATGCCGGTGAATT
ATCGGCAATCCTGAAGTTCGGTGCAGGCAATATGTTCACTGCCACAGACA
ACCAGAAAAAATTGGAGGATCTTAACTTGGATGATGTTTTGAATCATGCA
GAGGATCACGTTACTACTCCAGACTTAGGAGAGTCGCATCTTGGCGGTGA
AGAGTTTTTGAAGCAATTCGAAGTGACTGATTATAAAGCTGATATAGATT
GGGATGATATCATTCCAGAAGAAGAACTAAAAAAACTCCAAGATGAAGAG
CAGAAACGCAAGGATGAAGAATATGTTAAAGAACAACTTGAAATGATGAA
TAGAAGGGATAACGCCCTAAAAAAAATAAAAAACAGTGTTAATGGTGATG
GGACCGCCGCAAACTCAGATTCTGACGACGATAGTACTTCCAGATCTTCT
AGAAGAAGAGCAAGAGCTAATGACATGGACTCTATTGGTGAATCGGAGGT
GAGAGCTTTGTATAAAGCTATTTTAAAATTTGGCAACCTAAAGGAGATTT
TGGACGAGTTGATTGCAGATGGAACCCTGCCGGTCAAATCATTTGAAAAA
TACGGTGAAACTTACGATGAAATGATGGAAGCAGCTAAAGACTGTGTACA
CGAGGAAGAGAAAAATAGAAAAGAAATCTTGGAGAAACTTGAGAAGCATG
CTACCGCCTATAGAGCAAAGCTAAAAAGCGGTGAAATAAAAGCAGAGAAC
CAACCAAAGGATAATCCGTTGACTAGATTATCTTTAAAAAAAAGAGAAAA
GAAAGCCGTCCTTTTCAACTTTAAAGGTGTTAAATCTTTAAACGCCGAAT
CTTTACTAAGTAGAGTGGAGGATTTAAAGTACTTGAAGAACTTGATAAAT
TCAAACTACAAAGATGATCCATTAAAGTTTAGCCTAGGCAACAACACACC
TAAGCCTGTACAGAATTGGTCATCTAATTGGACGAAAGAGGAAGATGAGA
AGCTATTGATTGGTGTTTTCAAATATGGATATGGTTCCTGGACACAAATA
AGAGACGATCCATTTCTAGGCATTACTGATAAAATATTCTTGAATGAAGT
TCACAATCCGGTAGCAAAAAAGTCGGCAAGCTCCTCTGATACAACACCAA
CACCCTCAAAGAAAGGAAAGGGAATAACGGGTTCTTCAAAAAAAGTACCT
GGTGCCATTCACTTGGGCAGAAGAGTTGATTATCTATTATCCTTCTTAAG
AGGAGGCCTAAATACAAAGAGTCCCAGTGCTGACATAGGCTCGAAAAAAC
TCCCTACTGGTCCTTCCAAAAAGAGACAAAGAAAACCTGCTAATCACAGC
AAATCCATGACTCCAGAAATTACAAGTTCTGAGCCTGCAAATGGCCCGCC
AAGCAAACGTATGAAAGCCCTCCCAAAAGGTCCTGCCGCCTTAATAAATA
ACACGAGATTGTCTCCAAACAGCCCCACGCCCCCATTAAAGTCCAAGGTT
TCCAGAGATAATGGTACAAGGCAATCAAGCAACCCCAGTAGCGGCAGCGC
TCACGAAAAAGAATATGACAGCATGGATGAAGAAGACTGTCGACACACAA
TGAGTGCAATACGTACTTCTTTGAAGCGACTTAGAAGAGGCGGCAAAAGT
CTTGATCGTAAGGAATGGGCCAAAATTTTGAAGACCGAATTAACTACGAT
TGGTAATCATATCGAATCGCAAAAGGGCTCATCGAGAAAGGCAAGCCCAG
AAAAATATCGCAAGCACCTTTGGTCTTACAGTGCCAACTTTTGGCCTGCC
GACGTTAAGAGTACAAAGCTGATGGCAATGTACGACAAGATAACAGAGTC
TCAAAAGAAGTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAAKDISTEVLQNPELYGL
RRSHRAAAHQQNYFNDSDDEDDEDNIKQSRRKRMTTIEDDEDEFEDEEGE
EDSGEDEDEEDFEEDDDYYGSPIKQNRSKPKSRTKSKSKSKPKSQSEKQS
TVKIPTRFSNRQNKTVNYNIDYSDDDLLESEDDYGSEEALSEENVHEASA
NPQPEDFHGIDIVINHRLKTSLEEGKVLEKTVPDLNNCKENYEFLIKWTD
ESHLHNTWETYESIGQVRGLKRLDNYCKQFIIEDQQVRLDPYVTAEDIEI
MDMERERRLDEFEEFHVPERIIDSQRASLEDGTSQLQYLVKWRRLNYDEA
TWENATDIVKLAPEQVKHFQNRENSKILPQYSSNYTSQRPRFEKLSVQPP
FIKGGELRDFQLTGINWMAFLWSKGDNGILADEMGLGKTVQTVAFISWLI
FARRQNGPHIIVVPLSTMPAWLDTFEKWAPDLNCICYMGNQKSRDTIREY
EFYTNPRAKGKKTMKFNVLLTTYEYILKDRAELGSIKWQFMAVDEAHRLK
NAESSLYESLNSFKVANRMLITGTPLQNNIKELAALVNFLMPGRFTIDQE
IDFENQDEEQEEYIHDLHRRIQPFILRRLKKDVEKSLPSKTERILRVELS
DVQTEYYKNILTKNYSALTAGAKGGHFSLLNIMNELKKASNHPYLFDNAE
ERVLQKFGDGKMTRENVLRGLIMSSGKMVLLDQLLTRLKKDGHRVLIFSQ
MVRMLDILGDYLSIKGINFQRLDGTVPSAQRRISIDHFNSPDSNDFVFLL
STRAGGLGINLMTADTVVIFDSDWNPQADLQAMARAHRIGQKNHVMVYRL
VSKDTVEEEVLERARKKMILEYAIISLGVTDGNKYTKKNEPNAGELSAIL
KFGAGNMFTATDNQKKLEDLNLDDVLNHAEDHVTTPDLGESHLGGEEFLK
QFEVTDYKADIDWDDIIPEEELKKLQDEEQKRKDEEYVKEQLEMMNRRDN
ALKKIKNSVNGDGTAANSDSDDDSTSRSSRRRARANDMDSIGESEVRALY
KAILKFGNLKEILDELIADGTLPVKSFEKYGETYDEMMEAAKDCVHEEEK
NRKEILEKLEKHATAYRAKLKSGEIKAENQPKDNPLTRLSLKKREKKAVL
FNFKGVKSLNAESLLSRVEDLKYLKNLINSNYKDDPLKFSLGNNTPKPVQ
NWSSNWTKEEDEKLLIGVFKYGYGSWTQIRDDPFLGITDKIFLNEVHNPV
AKKSASSSDTTPTPSKKGKGITGSSKKVPGAIHLGRRVDYLLSFLRGGLN
TKSPSADIGSKKLPTGPSKKRQRKPANHSKSMTPEITSSEPANGPPSKRM
KALPKGPAALINNTRLSPNSPTPPLKSKVSRDNGTRQSSNPSSGSAHEKE
YDSMDEEDCRHTMSAIRTSLKRLRRGGKSLDRKEWAKILKTELTTIGNHI
ESQKGSSRKASPEKYRKHLWSYSANFWPADVKSTKLMAMYDKITESQKK*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P1 CHD1 yeast86.38 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU8178
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.; Estimated MW of expressed protein : 195060 Daltons
• Low Temp: No