cDNA Clone PIAS1

• Plasmid: pGEX6P-1-PIAS1
• Parent Plasmid: pGEX6P1
• Expressed: GST-PIAS1
• Genbank: BAC35902
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCATGGCGGACAGTGCGGAACTAAAGCAAATGGTTATGAGCCTTAG
AGTTTCTGAACTCCAAGTACTGTTGGGCTACGCTGGGAGGAACAAGCACG
GACGCAAACACGAACTTCTTACAAAAGCCCTGCATTTGTTAAAGGCTGGC
TGTAGTCCTGCTGTACAAATGAAAATTAAAGAACTCTACAGGAGGCGGTT
CCCTCAGAAAATTATGACGCCTGCGGACTTGTCTATCCCCAACGTACATT
CAAGTCCTATGCCTCCGACTCTTTCTCCATCCACCATTCCACAGCTCACT
TATGATGGCCACCCTGCATCATCCCCACTACTCCCTGTTTCTCTTCTGGG
ACCCAAACATGAACTGGAACTCCCACATCTCACGTCAGCGCTGCACCCAG
TCCACCCGGACATAAAGCTGCAGAAGCTACCATTCTATGACCTGTTGGAT
GAACTGATCAAGCCCACCAGTCTAGCTTCAGACAACAGCCAGCGCTTTCG
GGAAACCTGTTTTGCATTTGCCTTGACACCACAACAGGTGCAGCAGATCA
GCAGCTCCATGGATATTTCTGGGACCAAATGTGACTTCACAGTGCAGGTC
CAATTAAGGTTTTGTTTATCAGAAACCAGTTGTCCACAAGAAGATCACTT
CCCACCCAACCTTTGTGTAAAAGTGAATACAAAACCTTGCAGCCTTCCAG
GTTACCTTCCACCTACTAAAAACGGTGTGGAACCAAAGCGACCTAGCCGA
CCAATTAATATCACCTCACTTGTCCGATTGTCCACGACAGTACCAAATAC
CATTGTTGTTTCTTGGACTGCAGAAATTGGAAGAACCTATTCCATGGCAG
TATATCTTGTAAAACAGTTGTCCTCAACAGTTCTTCTTCAGAGGTTACGA
GCAAAGGGAATAAGGAATCCGGATCATTCTAGAGCTTTAATTAAAGAGAA
GTTAACTGCAGATCCAGATAGTGAGATAGCTACTACCAGCCTACGGGTTT
CGCTGCTGTGTCCACTTGGGAAAATGCGACTGACAATCCCCTGTCGGGCA
CTTACCTGCTCCCACCTTCAGTGTTTTGATGCAACTCTTTACATTCAAAT
GAATGAGAAAAAACCAACATGGGTTTGTCCTGTCTGTGATAAGAAGGCCC
CATATGAACACCTTATTATTGACGGGTTGTTTATGGAAATTCTAAAGTAC
TGCACAGACTGTGACGAGATACAGTTTAAGGAGGATGGCTCGTGGGCTCC
AATGAGGTCAAAGAAGGAGGTTCAAGAAGTCACTGCCTCCTACAATGGAG
TTGATGGTTGCTTGAGCTCCACATTGGAGCATCAGGTAGCGTCCCACAAC
CAGTCCTCAAATAAAAACAAGAAAGTCGAGGTCATTGACCTAACCATTGA
CAGCTCGTCAGATGAAGAGGAGGAAGAACCCCCTGCCAAGAGGACCTGTC
CTTCCCTGTCTCCTACGTCACCACTAAGTAATAAAGGCATTTTAAGTCTT
CCTCATCAAGCCTCGCCTGTGTCCCGCACCCCAAGCCTTCCTGCTGTAGA
TACAAGCTACATCAACACCTCCCTCATCCAGGACTACAGGCACCCCTTCC
ACATGACGCCTATGCCTTATGACTTACAAGGATTAGATTTCTTTCCTTTC
TTATCAGGAGACAATCAGCATTACAACACCTCCCTGCTAGCCGCAGCTGC
AGCGGCGGTCTCAGATGACCAGGACCTCCTGCACTCCTCCCGGTTTTTCC
CGTATACCTCCTCGCAGATGTTTCTCGACCAGCTAAGTGCAGGAGGGAGC
ACATCTCTGCCAGCCACCAACGGAAGCAGTAGCGGCAGCAACAGCAGCCT
TGTGTCTTCCAACAGTCTGAGAGAGAGCCATGGCCATGGTGTGGCCAGCA
GGAGCAGCGCAGACACAGCGTCCATCTTTGGCATCATACCAGACATTATC
TCATTGGACTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMADSAELKQMVMSLRVSEL
QVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKELYRRRFPQKI
MTPADLSIPNVHSSPMPPTLSPSTIPQLTYDGHPASSPLLPVSLLGPKHE
LELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASDNSQRFRETCF
AFALTPQQVQQISSSMDISGTKCDFTVQVQLRFCLSETSCPQEDHFPPNL
CVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVS
WTAEIGRTYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSRALIKEKLTAD
PDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDATLYIQMNEKK
PTWVCPVCDKKAPYEHLIIDGLFMEILKYCTDCDEIQFKEDGSWAPMRSK
KEVQEVTASYNGVDGCLSSTLEHQVASHNQSSNKNKKVEVIDLTIDSSSD
EEEEEPPAKRTCPSLSPTSPLSNKGILSLPHQASPVSRTPSLPAVDTSYI
NTSLIQDYRHPFHMTPMPYDLQGLDFFPFLSGDNQHYNTSLLAAAAAAVS
DDQDLLHSSRFFPYTSSQMFLDQLSAGGSTSLPATNGSSSGSNSSLVSSN
SLRESHGHGVASRSSADTASIFGIIPDIISLD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8105
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; Estimated MW of expressed protein : 98378 Daltons