cDNA Clone RING H2

• Plasmid: pEBG6P-RING H2
• Parent Plasmid: pEBG6P
• Expressed: GST-RING H2
• Genbank: AAO72970
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGATTCTCTACCAGATGAATTTTTTGTGAGGCATCCTGCTGT
GGAGGATCAGAGGAAGGAAGAAACTGAGAATAAGCTAGAAAAATCATCTG
GTCAACTGAACAAACAGGAAAATGACATACCTACTGATCTTGTCCCTGTT
AACCTACTATTAGAAGTGAAGAAGTTATTAAATGCAATTAATACTCTACC
AAAAGGTGTGGTTCCTCACATTAAGAAGTTCTTACAAGAAGATTTTTCCT
TCCAAACTATGCAGAGAGAAGTTGCAGCTAACAGCCAGAATGGTGAGGAA
ATTGTTCCTGCTTTGACTTTACGTTTCTTGATTACACAGCTAGAAGCAGC
ACTTAGGAACATTCAAGCTGGCAATTATACCGCACACCAGATTAATATTG
GTTATTATTTGACATTACTGTTTTTATATGGAGTAGCACTCACTGAAAGA
GGAAAGAAAGAGGATTATACAGAAGCTGAGAATAAATTTCTGGTGATGAA
GATGATGATCCAAGAAAATGAAATTTGTGAAAACTTTATGTCTTTAGTTT
ATTTTGGACGTGGTTTACTGCGATGTGCTCAAAAGAGATATAATGGAGGA
CTGCTAGAATTTCATAAAAGCTTACAAGAAATTGGAGACAAAAATGACCA
TTGGTTTGACATAGATCCTACAGAAGATGAAGATTTACCTACAACTTTTA
AAGATCTTCTTAATAATTTTATCAAGACAACTGAAAGCAATATAATGAAG
CAGACGATTTGTAGTTACCTAGATTGTGAACGATCTTGTGAAGCTGACAT
TTTGAAGAACACCAGTTATAAGGGATTTTTTCAGTTAATGTGCAGTAAAA
GTTGCTGTGTTTATTTCCATAAAATTTGCTGGAAAAAGTTCAAGAATTTA
AAGTATCCAGAGTTTTAGGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMDSLPDEFFVRHP
AVEDQRKEETENKLEKSSGQLNKQENDIPTDLVPVNLLLEVKKLLNAINT
LPKGVVPHIKKFLQEDFSFQTMQREVAANSQNGEEIVPALTLRFLITQLE
AALRNIQAGNYTAHQINIGYYLTLLFLYGVALTERGKKEDYTEAENKFLV
MKMMIQENEICENFMSLVYFGRGLLRCAQKRYNGGLLEFHKSLQEIGDKN
DHWFDIDPTEDEDLPTTFKDLLNNFIKTTESNIMKQTICSYLDCERSCEA
DILKNTSYKGFFQLMCSKSCCVYFHKICWKKFKNLKYPEF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8058
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression.; Estimated MW of expressed protein : 62671 Daltons