cDNA Clone RING H2

• Plasmid: pGEX6P-1-RING H2
• Parent Plasmid: pGEX6P1
• Expressed: GST-RING H2
• Genbank: AAO72970
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGATTCTCTACCAGATGAATTTTTTGTGAGGCATCCTGCTGT
GGAGGATCAGAGGAAGGAAGAAACTGAGAATAAGCTAGAAAAATCATCTG
GTCAACTGAACAAACAGGAAAATGACATACCTACTGATCTTGTCCCTGTT
AACCTACTATTAGAAGTGAAGAAGTTATTAAATGCAATTAATACTCTACC
AAAAGGTGTGGTTCCTCACATTAAGAAGTTCTTACAAGAAGATTTTTCCT
TCCAAACTATGCAGAGAGAAGTTGCAGCTAACAGCCAGAATGGTGAGGAA
ATTGTTCCTGCTTTGACTTTACGTTTCTTGATTACACAGCTAGAAGCAGC
ACTTAGGAACATTCAAGCTGGCAATTATACCGCACACCAGATTAATATTG
GTTATTATTTGACATTACTGTTTTTATATGGAGTAGCACTCACTGAAAGA
GGAAAGAAAGAGGATTATACAGAAGCTGAGAATAAATTTCTGGTGATGAA
GATGATGATCCAAGAAAATGAAATTTGTGAAAACTTTATGTCTTTAGTTT
ATTTTGGACGTGGTTTACTGCGATGTGCTCAAAAGAGATATAATGGAGGA
CTGCTAGAATTTCATAAAAGCTTACAAGAAATTGGAGACAAAAATGACCA
TTGGTTTGACATAGATCCTACAGAAGATGAAGATTTACCTACAACTTTTA
AAGATCTTCTTAATAATTTTATCAAGACAACTGAAAGCAATATAATGAAG
CAGACGATTTGTAGTTACCTAGATTGTGAACGATCTTGTGAAGCTGACAT
TTTGAAGAACACCAGTTATAAGGGATTTTTTCAGTTAATGTGCAGTAAAA
GTTGCTGTGTTTATTTCCATAAAATTTGCTGGAAAAAGTTCAAGAATTTA
AAGTATCCAGAGTTTTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMDSLPDEFFVRHPAVEDQR
KEETENKLEKSSGQLNKQENDIPTDLVPVNLLLEVKKLLNAINTLPKGVV
PHIKKFLQEDFSFQTMQREVAANSQNGEEIVPALTLRFLITQLEAALRNI
QAGNYTAHQINIGYYLTLLFLYGVALTERGKKEDYTEAENKFLVMKMMIQ
ENEICENFMSLVYFGRGLLRCAQKRYNGGLLEFHKSLQEIGDKNDHWFDI
DPTEDEDLPTTFKDLLNNFIKTTESNIMKQTICSYLDCERSCEADILKNT
SYKGFFQLMCSKSCCVYFHKICWKKFKNLKYPEF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8055
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.; Estimated MW of expressed protein : 62024 Daltons