cDNA Clone RAS

• Plasmid: pEBG6P-RAS
• Parent Plasmid: pEBG6P
• Expressed: GST-RAS
• Genbank: P01112
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCACGGAATATAAGCTGGTGGTGGTGGGCGCCGGCGGTGTGGGCAA
GAGTGCGCTGACCATCCAGCTGATCCAGAACCATTTTGTGGACGAATACG
ACCCCACTATAGAGGATTCCTACCGGAAGCAGGTGGTCATTGATGGGGAG
ACGTGCCTGTTGGACATCCTGGATACCGCCGGCCAGGAGGAGTACAGCGC
CATGCGGGACCAGTACATGCGCACCGGGGAGGGCTTCCTGTGTGTGTTTG
CCATCAACAACACCAAGTCTTTTGAGGACATCCACCAGTACAGGGAGCAG
ATCAAACGGGTGAAGGACTCGGATGACGTGCCCATGGTGCTGGTGGGGAA
CAAGTGTGACCTGGCTGCACGCACTGTGGAATCTCGGCAGGCTCAGGACC
TCGCCCGAAGCTACGGCATCCCCTACATCGAGACCTCGGCCAAGACCCGG
CAGGGAGTGGAGGATGCCTTCTACACGTTGGTGCGTGAGATCCGGCAGCA
CAAGCTGCGGAAGCTGAACCCTCCTGATGAGAGTGGCCCCGGCTGCATGA
GCTGCAAGTGTGTGCTCTCCTGAGAATTCAAAGGCCTACGTCGACGAGCT
CACTAGTCGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTEYKLVVVGAGGV
GKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEY
SAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLV
GNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIR
QHKLRKLNPPDESGPGCMSCKCVLS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8020
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST tag for mammalian expression.; Estimated MW of expressed protein : 48553 Daltons.