cDNA Clone ICK (mouse)

• Plasmid: pCMV-HA ICK (mouse) T157A Y159F
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-ICK (mouse) T157A Y159F
• Genbank: AAH28863
• Tag: HA
• Species: Mouse

Sequence of Insert

GCGGCCGCGATGAATAGATACACAACGATCAAGCAGCTTGGGGATGGGAC
CTACGGCTCTGTCCTGCTGGGGAGAAGCATTGAGTCTGGAGAACTGATTG
CCATTAAAAAAATGAAAAGAAAGTTTTACTCCTGGGAAGAATGCATGAAC
CTTCGGGAGGTTAAGTCTTTAAAGAAGCTTAATCATGCCAATATCGTAAA
GTTAAAAGAGGTTATCAGGGAAAACGACCATCTTTACTTTATCTTCGAGT
ACATGAAGGAAAACCTTTACCAGCTCATTAAAGAAAGAAATAAGTTGTTT
CCAGAGTCTGCTATAAGGAATATAATGTACCAGATATTGCAAGGACTGGC
ATTCATTCACAAACACGGTTTCTTCCACCGGGACTTAAAACCCGAGAACC
TCCTCTGCATGGGACCAGAACTTGTGAAAATCGCCGACTTTGGATTGGCT
CGAGAAATCCGATCAAGACCTCCGTACGCAGACTTTGTGTCTACTAGATG
GTACAGGGCTCCAGAGGTACTGCTGAGATCCACCAACTACAGCTCCCCCA
TTGACATCTGGGCGGTAGGCTGCATCATGGCGGAGGTGTACACGCTCCGG
CCCCTTTTCCCTGGGGCCAGTGAAATTGACACAATTTTCAAAATTTGCCA
AGTGTTGGGAACACCAAAGAAGACTGACTGGCCTGAAGGCTACCAGCTGT
CAAGTGCTATGAATTTCCTCTGGCCCCAGTGCATACCCAATAACTTAAAG
ACTCTAATCCCAAATGCTAGCAGCGAAGCCATTCAGCTCCTGAGAGACTT
GCTTCAGTGGGATCCCAAGAAACGACCAACCGCTAGTCAGGCTCTTCGAT
ATCCTTACTTCCAGATCGGACACCCACTGGGCATCATCAGCAAGGACTCA
GGAAAACCACAGAGAGAGGTCCAGGACAAGACAGGCCCTCCTCCTTACAT
CAAGCCAGCCCCTCCTGCCCAGGCCCCGGCCAAGGCATATACGCTGATTT
CTTCACGACCAAGCCAAGCCAGTCAGCCCCCTCAGCATTCTGTGCACCCC
TACAAAGGCGATGTCTCTAGGACTGAGCAGCTGAGCCATGTTCAGGAGGG
CAAGCCAAGCCCTCCATTCTTCCCATCCCTCCACAATAAGAATCTTCAGC
CAAAAATCCTAGCCAGCCTGGAACAGAAAAATGGTGAAATCAAGCCAAAG
AGCAGGAGAAGATGGGGTCTCATCTCCAGGTCGACAAAGGGTTCTGACGA
CTGGGCCGACTTGGATGACTTAGACTTCAGTCCCTCTCTCACCAGGATAG
ATGTAAAAAACAAGAAGAGACAGAGTGATGACACCCTCTGCAGGTTTGAA
AGTGTTCTGGACCTGAAGCCATCTGAGTCAGTGGGTACAGGAACCACTGT
CTCCACGCAGGCTTCCTCGCAGAGGAGAGACACGCCGACCCTGCAGTCCT
CAGCAAAGCAGCACTACTTGAAGCACTCTCGCTACTTGCCTGGAATAAAT
ATAAGAAATGGCGTGCTCCCAAATCCAGGCAAGGACTTTCTTCCATCAAA
TTCATGGTCCAGTTCCGGCTTGTCTGGAAAATCTTCAGGAACAGTATCAG
TAGTGAGCAAAATAACTTCAGTTGGCTCAGGCTCTGCGAGCTCTAGTGGA
CTGACTGGAAGCTACATCCCTTCCTTTCTGAAAAAGGAAATCGGTTCTGT
CATGCAGAGGGTTCAGCTGGCGCCCCTCGCAGCCCCTCCCCCCGGCTACT
CTTCCCTCAAGGCCGTGAGACCTCACCCGGGGAGGCCTTTCTTTCACACC
CAGCCCAGGAGCACGCCCGGCTTGATCCCCCGACCTCCAGCTGCTCAGCC
TGTGCACGGCAGGATAGACTGGTCTTCCAAGTACCCATCCCGGCGGTGAG
CGGCCGC

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAMNRYTTIKQLGDGTYGSVLLGRSIESG
ELIAIKKMKRKFYSWEECMNLREVKSLKKLNHANIVKLKEVIRENDHLYF
IFEYMKENLYQLIKERNKLFPESAIRNIMYQILQGLAFIHKHGFFHRDLK
PENLLCMGPELVKIADFGLAREIRSRPPYADFVSTRWYRAPEVLLRSTNY
SSPIDIWAVGCIMAEVYTLRPLFPGASEIDTIFKICQVLGTPKKTDWPEG
YQLSSAMNFLWPQCIPNNLKTLIPNASSEAIQLLRDLLQWDPKKRPTASQ
ALRYPYFQIGHPLGIISKDSGKPQREVQDKTGPPPYIKPAPPAQAPAKAY
TLISSRPSQASQPPQHSVHPYKGDVSRTEQLSHVQEGKPSPPFFPSLHNK
NLQPKILASLEQKNGEIKPKSRRRWGLISRSTKGSDDWADLDDLDFSPSL
TRIDVKNKKRQSDDTLCRFESVLDLKPSESVGTGTTVSTQASSQRRDTPT
LQSSAKQHYLKHSRYLPGINIRNGVLPNPGKDFLPSNSWSSSGLSGKSSG
TVSVVSKITSVGSGSASSSGLTGSYIPSFLKKEIGSVMQRVQLAPLAAPP
PGYSSLKAVRPHPGRPFFHTQPRSTPGLIPRPPAAQPVHGRIDWSSKYPS
RR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8009
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA tag for mammalian expression.; Estimated MW of expressed protein : 72864 Daltons