cDNA Clone ICK (mouse)

• Plasmid: pGEX6P-1-ICK (mouse)
• Parent Plasmid: pGEX6P2
• Expressed: GST-ICK (mouse)
• Genbank: AAH28863
• Tag: GST
• Species: Mouse

Sequence of Insert

GCGGCCGCGATGAATAGATACACAACGATCAAGCAGCTTGGGGATGGGAC
CTACGGCTCTGTCCTGCTGGGGAGAAGCATTGAGTCTGGAGAACTGATTG
CCATTAAAAAAATGAAAAGAAAGTTTTACTCCTGGGAAGAATGCATGAAC
CTTCGGGAGGTTAAGTCTTTAAAGAAGCTTAATCATGCCAATATCGTAAA
GTTAAAAGAGGTTATCAGGGAAAACGACCATCTTTACTTTATCTTCGAGT
ACATGAAGGAAAACCTTTACCAGCTCATTAAAGAAAGAAATAAGTTGTTT
CCAGAGTCTGCTATAAGGAATATAATGTACCAGATATTGCAAGGACTGGC
ATTCATTCACAAACACGGTTTCTTCCACCGGGACTTAAAACCCGAGAACC
TCCTCTGCATGGGACCAGAACTTGTGAAAATCGCCGACTTTGGATTGGCT
CGAGAAATCCGATCAAGACCTCCGTACACAGACTATGTGTCTACTAGATG
GTACAGGGCTCCAGAGGTACTGCTGAGATCCACCAACTACAGCTCCCCCA
TTGACATCTGGGCGGTAGGCTGCATCATGGCGGAGGTGTACACGCTCCGG
CCCCTTTTCCCTGGGGCCAGTGAAATTGACACAATTTTCAAAATTTGCCA
AGTGTTGGGAACACCAAAGAAGACTGACTGGCCTGAAGGCTACCAGCTGT
CAAGTGCTATGAATTTCCTCTGGCCCCAGTGCATACCCAATAACTTAAAG
ACTCTAATCCCAAATGCTAGCAGCGAAGCCATTCAGCTCCTGAGAGACTT
GCTTCAGTGGGATCCCAAGAAACGACCAACCGCTAGTCAGGCTCTTCGAT
ATCCTTACTTCCAGATCGGACACCCACTGGGCATCATCAGCAAGGACTCA
GGAAAACCACAGAGAGAGGTCCAGGACAAGACAGGCCCTCCTCCTTACAT
CAAGCCAGCCCCTCCTGCCCAGGCCCCGGCCAAGGCATATACGCTGATTT
CTTCACGACCAAGCCAAGCCAGTCAGCCCCCTCAGCATTCTGTGCACCCC
TACAAAGGCGATGTCTCTAGGACTGAGCAGCTGAGCCATGTTCAGGAGGG
CAAGCCAAGCCCTCCATTCTTCCCATCCCTCCACAATAAGAATCTTCAGC
CAAAAATCCTAGCCAGCCTGGAACAGAAAAATGGTGAAATCAAGCCAAAG
AGCAGGAGAAGATGGGGTCTCATCTCCAGGTCGACAAAGGGTTCTGACGA
CTGGGCCGACTTGGATGACTTAGACTTCAGTCCCTCTCTCACCAGGATAG
ATGTAAAAAACAAGAAGAGACAGAGTGATGACACCCTCTGCAGGTTTGAA
AGTGTTCTGGACCTGAAGCCATCTGAGTCAGTGGGTACAGGAACCACTGT
CTCCACGCAGGCTTCCTCGCAGAGGAGAGACACGCCGACCCTGCAGTCCT
CAGCAAAGCAGCACTACTTGAAGCACTCTCGCTACTTGCCTGGAATAAAT
ATAAGAAATGGCGTGCTCCCAAATCCAGGCAAGGACTTTCTTCCATCAAA
TTCATGGTCCAGTTCCGGCTTGTCTGGAAAATCTTCAGGAACAGTATCAG
TAGTGAGCAAAATAACTTCAGTTGGCTCAGGCTCTGCGAGCTCTAGTGGA
CTGACTGGAAGCTACATCCCTTCCTTTCTGAAAAAGGAAATCGGTTCTGT
CATGCAGAGGGTTCAGCTGGCGCCCCTCGCAGCCCCTCCCCCCGGCTACT
CTTCCCTCAAGGCCGTGAGACCTCACCCGGGGAGGCCTTTCTTTCACACC
CAGCCCAGGAGCACGCCCGGCTTGATCCCCCGACCTCCAGCTGCTCAGCC
TGTGCACGGCAGGATAGACTGGTCTTCCAAGTACCCATCCCGGCGGTGAG
CGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMNRYTTIK
QLGDGTYGSVLLGRSIESGELIAIKKMKRKFYSWEECMNLREVKSLKKLN
HANIVKLKEVIRENDHLYFIFEYMKENLYQLIKERNKLFPESAIRNIMYQ
ILQGLAFIHKHGFFHRDLKPENLLCMGPELVKIADFGLAREIRSRPPYTD
YVSTRWYRAPEVLLRSTNYSSPIDIWAVGCIMAEVYTLRPLFPGASEIDT
IFKICQVLGTPKKTDWPEGYQLSSAMNFLWPQCIPNNLKTLIPNASSEAI
QLLRDLLQWDPKKRPTASQALRYPYFQIGHPLGIISKDSGKPQREVQDKT
GPPPYIKPAPPAQAPAKAYTLISSRPSQASQPPQHSVHPYKGDVSRTEQL
SHVQEGKPSPPFFPSLHNKNLQPKILASLEQKNGEIKPKSRRRWGLISRS
TKGSDDWADLDDLDFSPSLTRIDVKNKKRQSDDTLCRFESVLDLKPSESV
GTGTTVSTQASSQRRDTPTLQSSAKQHYLKHSRYLPGINIRNGVLPNPGK
DFLPSNSWSSSGLSGKSSGTVSVVSKITSVGSGSASSSGLTGSYIPSFLK
KEIGSVMQRVQLAPLAAPPPGYSSLKAVRPHPGRPFFHTQPRSTPGLIPR
PPAAQPVHGRIDWSSKYPSRR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3990
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.; Calc Mol Weight : 98333 Daltons