cDNA Clone CDK17

• Plasmid: pEBG6P-CDK17
• Parent Plasmid: pEBG6P
• Expressed: GST CDK17
• Genbank: AAH33005.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGAAAAAATTTAAGAGAAGGCTATCCCTCACACTCCGAGGAAG
TCAGACTATTGATGAATCATTGTCTGAATTGGCTGAACAAATGACTATTG
AAGAAAACAGCAGCAAGGATAATGAGCCTATTGTGAAGAATGGCAGGCCT
CCAACGTCTCACAGTATGCATTCCTTCCTCCACCAGTACACAGGATCTTT
CAAGAAGCCCCCATTGCGGAGACCACACAGTGTTATTGGAGGGAGCCTTG
GCTCCTTCATGGCAATGCCCAGAAATGGAAGCAGATTAGATATTGTTCAT
GAAAATCTAAAAATGGGATCAGATGGTGAGAGTGACCAAGCTTCTGGGAC
ATCATCTGATGAAGTCCAGTCACCTACAGGTGTTTGTCTCAGAAATCGTA
TACATAGACGGATCTCAATGGAGGATTTAAATAAGCGGTTATCACTGCCT
GCAGACATCAGAATACCTGATGGATATCTTGAAAAGTTGCAGATAAACAG
TCCACCATTTGACCAACCAATGAGTCGAAGGTCTCGTAGAGCTTCCTTAT
CAGAAATTGGCTTTGGAAAAATGGAAACCTACATCAAATTGGAAAAGCTT
GGAGAGGGTACATATGCAACAGTATATAAAGGAAGAAGTAAATTGACAGA
GAATTTGGTGGCATTAAAAGAGATCCGATTGGAACATGAAGAAGGTGCAC
CCTGCACAGCTATAAGAGAAGTTTCACTATTAAAGGATTTAAAACATGCA
AATATAGTAACCTTACATGACATTGTTCACACAGATAAATCCTTGACTTT
GGTGTTTGAGTATCTGGATAAAGACCTGAAACAGTACATGGATGACTGTG
GAAACATCATGAGTATGCACAACGTAAAGCTGTTTCTGTACCAAATTCTA
CGTGGTTTGGCATATTGCCATAGAAGAAAGGTATTGCATCGAGACTTGAA
ACCACAGAACCTCCTCATTAATGAGAAAGGAGAATTAAAGCTAGCAGATT
TTGGACTAGCCCGAGCCAAGTCAGTTCCCACAAAGACCTACTCAAATGAA
GTTGTCACACTATGGTACCGGCCACCTGATGTGCTTCTTGGTTCCTCGGA
GTACTCAACACAGATTGACATGTGGGGTGTTGGTTGCATTTTCTTTGAAA
TGGCTTCTGGAAGACCTTTATTTCCAGGATCAACCGTGGAAGATGAACTG
CACTTAATTTTCCGACTGCTAGGAACTCCATCTCAGGAAACTTGGCCAGG
TATTTCTTCAAATGAGGAGTTCAAGAACTACAACTTTCCAAAATATAAAC
CACAGCCTCTAATTAACCACGCACCCAGGTTAGACTCTGAAGGAATTGAG
TTGATAACAAAATTTCTTCAGTATGAATCTAAGAAAAGGGTTTCAGCTGA
AGAGGCCATGAAACATGTGTACTTTCGAAGTCTGGGACCAAGAATACATG
CTTTACCAGAAAGTGTATCAATATTCAGTTTGAAAGAGATTCAGTTGCAA
AAGGACCCGGGTTTTCGAAATTCTTCTTATCCAGAGACAGGACATGGGAA
GAACAGAAGACAGAGCATGCTCTTTTAAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMKKFKRRLSLTLR
GSQTIDESLSELAEQMTIEENSSKDNEPIVKNGRPPTSHSMHSFLHQYTG
SFKKPPLRRPHSVIGGSLGSFMAMPRNGSRLDIVHENLKMGSDGESDQAS
GTSSDEVQSPTGVCLRNRIHRRISMEDLNKRLSLPADIRIPDGYLEKLQI
NSPPFDQPMSRRSRRASLSEIGFGKMETYIKLEKLGEGTYATVYKGRSKL
TENLVALKEIRLEHEEGAPCTAIREVSLLKDLKHANIVTLHDIVHTDKSL
TLVFEYLDKDLKQYMDDCGNIMSMHNVKLFLYQILRGLAYCHRRKVLHRD
LKPQNLLINEKGELKLADFGLARAKSVPTKTYSNEVVTLWYRPPDVLLGS
SEYSTQIDMWGVGCIFFEMASGRPLFPGSTVEDELHLIFRLLGTPSQETW
PGISSNEEFKNYNFPKYKPQPLINHAPRLDSEGIELITKFLQYESKKRVS
AEEAMKHVYFRSLGPRIHALPESVSIFSLKEIQLQKDPGFRNSSYPETGH
GKNRRQSMLF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU35699
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression; ; Estimated MW of expressed protein : 86998 Daltons