cDNA Clone CARD7

• Plasmid: pEBG6p-CARD7 (NLRP1)
• Parent Plasmid: pEBG6P
• Expressed: GST-CARD7
• Genbank: NM_033004.3
• Tag: GST
• Species: Human

Sequence of Insert

ACTAGTATGGCTGGCGGAGCCTGGGGCCGCCTGGCCTGTTACTTGGAGTTCCTGAAGAAGGAGGAGCTGAAGGAGTTCCAGCTTCTGCTCGCCAATAAAGCGCACTCCAGGAGCTCTTCGGGTGAGACACCCGCTCAGCCAGAGAAGACGAGTGGCATGGAGGTGGCCTCGTACCTGGTGGCTCAGTATGGGGAGCAGCGGGCCTGGGACCTAGCCCTCCATACCTGGGAGCAGATGGGGCTGAGGTCACTGTGCGCCCAAGCCCAGGAAGGGGCAGGCCACTCTCCCTCATTCCCCTACAGCCCAAGTGAACCCCACCTGGGGTCTCCCAGCCAACCCACCTCCACCGCAGTGCTAATGCCCTGGATCCATGAATTGCCGGCGGGGTGCACCCAGGGCTCAGAGAGAAGGGTTTTGAGACAGCTGCCTGACACATCTGGACGCCGCTGGAGAGAAATCTCTGCCTCACTCCTCTACCAAGCTCTTCCAAGCTCCCCAGACCATGAGTCTCCAAGCCAGGAGTCACCCAACGCCCCCACATCCACAGCAGTGCTGGGGAGCTGGGGATCCCCACCTCAGCCCAGCCTAGCACCCAGAGAGCAGGAGGCTCCTGGGACCCAATGGCCTCTGGATGAAACGTCAGGAATTTACTACACAGAAATCAGAGAAAGAGAGAGAGAGAAATCAGAGAAAGGCAGGCCCCCATGGGCAGCGGTGGTAGGAACGCCCCCACAGGCGCACACCAGCCTACAGCCCCACCACCACCCATGGGAGCCTTCTGTGAGAGAGAGCCTCTGTTCCACATGGCCCTGGAAAAATGAGGATTTTAACCAAAAATTCACACAGCTGCTACTTCTACAAAGATCTCACCCCAGAAGCCAAGATCCCCTGGTCAAGAGAAGCTGGCCTGATTATGTGGAGGAGAATCGAGGACATTTAATTGAGATCAGAGACTTATTTGGCCCAGGCCTGGATACCCAAGAACCTCGCATAGTCATACTGCAGGGGGCTGCTGGAATTGGGAAGTCAACACTGGCCAGGCAGGTGAAGGAAGCCTGGGGGAGAGGCCAGCTGTATGGGGACCGCTTCCAGCATGTCTTCTACTTCAGCTGCAGAGAGCTGGCCCAGTCCAAGGTGGTGAGTCTCGCTGAGCTCATCGGAAAAGATGGGACAGCCACTCCGGCTCCCATTAGACAGATCCTGTCTAGGCCAGAGCGGCTGCTCTTCATCCTCGATGGTGTAGATGAGCCAGGATGGGTCTTGCAGGAGCCGAGTTCTGAGCTCTGTCTGCACTGGAGCCAGCCACAGCCGGCGGATGCACTGCTGGGCAGTTTGCTGGGGAAAACTATACTTCCCGAGGCATCCTTCCTGATCACGGCTCGGACCACAGCTCTGCAGAACCTCATTCCTTCTTTGGAGCAGGCACGTTGGGTAGAGGTCCTGGGGTTCTCTGAGTCCAGCAGGAAGGAATATTTCTACAGATATTTCACAGATGAAAGGCAAGCAATTAGAGCCTTTAGGTTGGTCAAATCAAACAAAGAGCTCTGGGCCCTGTGTCTTGTGCCCTGGGTGTCCTGGCTGGCCTGCACTTGCCTGATGCAGCAGATGAAGCGGAAGGAAAAACTCACACTGACTTCCAAGACCACCACAACCCTCTGTCTACATTACCTTGCCCAGGCTCTCCAAGCTCAGCCATTGGGACCCCAGCTCAGAGACCTCTGCTCTCTGGCTGCTGAGGGCATCTGGCAAAAAAAGACCCTTTTCAGTCCAGATGACCTCAGGAAGCATGGGTTAGATGGGGCCATCATCTCCACCTTCTTGAAGATGGGTATTCTTCAAGAGCACCCCATCCCTCTGAGCTACAGCTTCATTCACCTCTGTTTCCAAGAGTTCTTTGCAGCAATGTCCTATGTCTTGGAGGATGAGAAGGGGAGAGGTAAACATTCTAATTGCATCATAGATTTGGAAAAGACGCTAGAAGCATATGGAATACATGGCCTGTTTGGGGCATCAACCACACGTTTCCTATTGGGCCTGTTAAGTGATGAGGGGGAGAGAGAGATGGAGAACATCTTTCACTGCCGGCTGTCTCAGGGGAGGAACCTGATGCAGTGGGTCCCGTCCCTGCAGCTGCTGCTGCAGCCACACTCTCTGGAGTCCCTCCACTGCTTGTACGAGACTCGGAACAAAACGTTCCTGACACAAGTGATGGCCCATTTCGAAGAAATGGGCATGTGTGTAGAAACAGACATGGAGCTCTTAGTGTGCACTTTCTGCATTAAATTCAGCCGCCACGTGAAGAAGCTTCAGCTGATTGAGGGCAGGCAGCACAGATCAACATGGAGCCCCACCATGGTAGTCCTGTTCAGGTGGGTCCCAGTCACAGATGCCTATTGGCAGATTCTCTTCTCCGTCCTCAAGGTCACCAGAAACCTGAAGGAGCTGGACCTAAGTGGAAACTCGCTGAGCCACTCTGCAGTGAAGAGTCTTTGTAAGACCCTGAGACGCCCTCGCTGCCTCCTGGAGACCCTGCGGTTGGCTGGCTGTGGCCTCACAGCTGAGGACTGCAAGGACCTTGCCTTTGGGCTGAGAGCCAACCAGACCCTGACCGAGCTGGACCTGAGCTTCAATGTGCTCACGGATGCTGGAGCCAAACACCTTTGCCAGAGACTGAGACAGCCGAGCTGCAAGCTACAGCGACTGCAGCTGGTCAGCTGTGGCCTCACGTCTGACTGCTGCCAGGACCTGGCCTCTGTGCTTAGTGCCAGCCCCAGCCTGAAGGAGCTAGACCTGCAGCAGAACAACCTGGATGACGTTGGCGTGCGACTGCTCTGTGAGGGGCTCAGGCATCCTGCCTGCAAACTCATACGCCTGGGGCTGGACCAGACAACTCTGAGTGATGAGATGAGGCAGGAACTGAGGGCCCTGGAGCAGGAGAAACCTCAGCTGCTCATCTTCAGCAGACGGAAACCAAGTGTGATGACCCCTACTGAGGGCCTGGATACGGGAGAGATGAGTAATAGCACATCCTCACTCAAGCGGCAGAGACTCGGATCAGAGAGGGCGGCTTCCCATGTTGCTCAGGCTAATCTCAAACTCCTGGACGTGAGCAAGATCTTCCCAATTGCTGAGATTGCAGAGGAAAGCTCCCCAGAGGTAGTACCGGTGGAACTCTTGTGCGTGCCTTCTCCTGCCTCTCAAGGGGACCTGCATACGAAGCCTTTGGGGACTGACGATGACTTCTGGGGCCCCACGGGGCCTGTGGCTACTGAGGTAGTTGACAAAGAAAAGAACTTGTACCGAGTTCACTTCCCTGTAGCTGGCTCCTACCGCTGGCCCAACACGGGTCTCTGCTTTGTGATGAGAGAAGCGGTGACCGTTGAGATTGAATTCTGTGTGTGGGACCAGTTCCTGGGTGAGATCAACCCACAGCACAGCTGGATGGTGGCAGGGCCTCTGCTGGACATCAAGGCTGAGCCTGGAGCTGTGGAAGCTGTGCACCTCCCTCACTTTGTGGCTCTCCAAGGGGGCCATGTGGACACATCCCTGTTCCAAATGGCCCACTTTAAAGAGGAGGGGATGCTCCTGGAGAAGCCAGCCAGGGTGGAGCTGCATCACATAGTTCTGGAAAACCCCAGCTTCTCCCCCTTGGGAGTCCTCCTGAAAATGATCCATAATGCCCTGCGCTTCATTCCCGTCACCTCTGTGGTGTTGCTTTACCACCGCGTCCATCCTGAGGAAGTCACCTTCCACCTCTACCTGATCCCAAGTGACTGCTCCATTCGGAAGGCCATAGATGATCTAGAAATGAAATTCCAGTTTGTGCGAATCCACAAGCCACCCCCGCTGACCCCACTTTATATGGGCTGTCGTTACACTGTGTCTGGGTCTGGTTCAGGGATGCTGGAAATACTCCCCAAGGAACTGGAGCTCTGCTATCGAAGCCCTGGAGAAGACCAGCTGTTCTCGGAGTTCTACGTTGGCCACTTGGGATCAGGGATCAGGCTGCAAGTGAAAGACAAGAAAGATGAGACTCTGGTGTGGGAGGCCTTGGTGAAACCAGGAGATCTCATGCCTGCAACTACTCTGATCCCTCCAGCCCGCATAGCCGTACCTTCACCTCTGGATGCCCCGCAGTTGCTGCACTTTGTGGACCAGTATCGAGAGCAGCTGATAGCCCGAGTGACATCGGTGGAGGTTGTCTTGGACAAACTGCATGGACAGGTGCTGAGCCAGGAGCAGTACGAGAGGGTGCTGGCTGAGAACACGAGGCCCAGCCAGATGCGGAAGCTGTTCAGCTTGAGCCAGTCCTGGGACCGGAAGTGCAAAGATGGACTCTACCAAGCCCTGAAGGAGACCCATCCTCACCTCATTATGGAACTCTGGGAGAAGGGCAGCAAAAAGGGACTCCTGCCACTCAGCAGCTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSMAGGAWGRLACYLEFLKKEELKEFQLLLANKAHSRSSSGETPAQPEKTSGMEVASYLVAQYGEQRAWDLALHTWEQMGLRSLCAQAQEGAGHSPSFPYSPSEPHLGSPSQPTSTAVLMPWIHELPAGCTQGSERRVLRQLPDTSGRRWREISASLLYQALPSSPDHESPSQESPNAPTSTAVLGSWGSPPQPSLAPREQEAPGTQWPLDETSGIYYTEIREREREKSEKGRPPWAAVVGTPPQAHTSLQPHHHPWEPSVRESLCSTWPWKNEDFNQKFTQLLLLQRSHPRSQDPLVKRSWPDYVEENRGHLIEIRDLFGPGLDTQEPRIVILQGAAGIGKSTLARQVKEAWGRGQLYGDRFQHVFYFSCRELAQSKVVSLAELIGKDGTATPAPIRQILSRPERLLFILDGVDEPGWVLQEPSSELCLHWSQPQPADALLGSLLGKTILPEASFLITARTTALQNLIPSLEQARWVEVLGFSESSRKEYFYRYFTDERQAIRAFRLVKSNKELWALCLVPWVSWLACTCLMQQMKRKEKLTLTSKTTTTLCLHYLAQALQAQPLGPQLRDLCSLAAEGIWQKKTLFSPDDLRKHGLDGAIISTFLKMGILQEHPIPLSYSFIHLCFQEFFAAMSYVLEDEKGRGKHSNCIIDLEKTLEAYGIHGLFGASTTRFLLGLLSDEGEREMENIFHCRLSQGRNLMQWVPSLQLLLQPHSLESLHCLYETRNKTFLTQVMAHFEEMGMCVETDMELLVCTFCIKFSRHVKKLQLIEGRQHRSTWSPTMVVLFRWVPVTDAYWQILFSVLKVTRNLKELDLSGNSLSHSAVKSLCKTLRRPRCLLETLRLAGCGLTAEDCKDLAFGLRANQTLTELDLSFNVLTDAGAKHLCQRLRQPSCKLQRLQLVSCGLTSDCCQDLASVLSASPSLKELDLQQNNLDDVGVRLLCEGLRHPACKLIRLGLDQTTLSDEMRQELRALEQEKPQLLIFSRRKPSVMTPTEGLDTGEMSNSTSSLKRQRLGSERAASHVAQANLKLLDVSKIFPIAEIAEESSPEVVPVELLCVPSPASQGDLHTKPLGTDDDFWGPTGPVATEVVDKEKNLYRVHFPVAGSYRWPNTGLCFVMREAVTVEIEFCVWDQFLGEINPQHSWMVAGPLLDIKAEPGAVEAVHLPHFVALQGGHVDTSLFQMAHFKEEGMLLEKPARVELHHIVLENPSFSPLGVLLKMIHNALRFIPVTSVVLLYHRVHPEEVTFHLYLIPSDCSIRKAIDDLEMKFQFVRIHKPPPLTPLYMGCRYTVSGSGSGMLEILPKELELCYRSPGEDQLFSEFYVGHLGSGIRLQVKDKKDETLVWEALVKPGDLMPATTLIPPARIAVPSPLDAPQLLHFVDQYREQLIARVTSVEVVLDKLHGQVLSQEQYERVLAENTRPSQMRKLFSLSQSWDRKCKDGLYQALKETHPHLIMELWEKGSKKGLLPLSS*AAX

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU17873
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Pro 287 in NM_033004.3 is a Serin our clone from EST–needs correction if more work will go on with it.; ; Amplified from IMAGE:5756099 (N-ter); IMAGE:5211072 (C-ter)