cDNA Clone Glucose oxidase

• Plasmid: pGEX6P-1-Glucose oxidase (23-605)
• Parent Plasmid: pGEX6P1
• Species: Aspergillus niger

Sequence of Insert

ggatccAGCAATGGCATTGAAGCAAACCTCCTGACCGATCCCAACGATGT
CGCCGGCCGCACCGTCGACTACATCATCGCTGGTGGAGGTCTGACTGGGC
TCACCACCGCCGCCCGTCTGACTGAGGACCCCAACATCACCGTCCTCGTC
ATTGAAAGTGGCTCCTACGAGTCAGACAGGGGTGCTATCATCGAGGACCT
CAACGCCTACGGCGACATCTTTGGCAGCAGCGTCGACCATGCCTACGAGA
CCGTGACGCTCGCCACCAACAATCAGACTGCGCTGATCCGCTCCGGAAAT
GGTCTCGGTGGCTCTACCTTGGTCAATGGTGGCACTTGGACTCGCCCCCA
CAAAGCGCAAGTTGACTCATGGGAGACTGTCTTTGGCAATGAGGGCTGGA
ACTGGGACAACGTGGCTGCCTACTCCCTCCAGGCGGAGCGTGCTCGCGCA
CCCAACGCCAAACAGATCGCTGCTGGCCACTACTTCAACGCATCCTGTCA
TGGTGTCAACGGCACTGTCCATGCCGGACCCCGCGACACCGGCGATGACT
ATTCTCCCATCGTCCAGGCTCTCATGAGCGCTGTGGAAGACAGGGGCGTC
CCTACCAAGAAGGACTTTGGATGCGGTGACCCCCATGGTGTGTCCATGTT
CCCCAACACCTTGCACGAAGACCAAGTGCGCTCCGATGCCGCTCGCGAAT
GGCTCCTTCCCAACTACCAGCGTCCCAACTTGCAAGTCCTGACCGGACAG
TTTGTCGGAAAGGTCTTGCTCAACCAGAACACGACCACCCCTCGTGCCGT
CGGTGTGGAATTCGGCACGCACAAGGGCAACACCCACAACGTCTACGCGA
AGCACGAGGTCCTCCTGGCCGCTGGCTCCGCTATCTCTCCCACCATCCTC
GAATATTCCGGTATCGGCATGAAGTCCATCCTGGAGCCTCTTGGAATCGA
TACCGTCGTTGACCTTCCTGTCGGCCTGAACCTGCAGGACCAGACTACTG
CTACCGTCCGCTCGCGCATCACCTCCGCCGGTGCCGGACAGGGACAGGCT
GCTTGGTTCGCCACCTTCAACGAGACCTTTGGTGACTACGCCGAAAAGGC
GCATGAGCTGCTGAACACCAAGCTGGAGCAGTGGGCCGAAGAGGCCGTCG
CCCGTGGCGGATTCCACAACACCACCGCCTTGCTCATCCAGTACGAGAAC
TACCGCGACTGGATCGTCAACCACAATGTCGCGTACGCGGAACTTTTCCT
CGACACGGCCGGAGTGGCCAGCTTTGACGTCTGGGACCTCCTTCCCTTCA
CGAGAGGATACGTCCATATTCTCGACAAGGACCCCTACCTCCGCCACTTC
GCCTACGACCCTCAGTACTTCCTCAACGAGCTGGACCTGCTCGGTCAGGC
GTCCGCTCTTCAGCTGGCCCGGAACATCTCCAACTCGGGTGCCATGCAGA
CGTACTTCGCAGGAGAGACCATTCCCGGTGATAACCTCGCGTATGATGCC
GATTTGATGGCCTGGGCTGAGTATCTCCCGTACCACTTCCGTCCCAACTA
CCATGGAGTGGGAACGTGCTCCATGATGTCGAGGGAGATGGGCGGTGTGG
TCGACAATGCTGCCCGTGTGTATGGTGTGCAGGGGCTGCGGGTCATTGAT
GGTTCTATTCCGCCTACGCAGGTCTCGTCCCATGTGATGACGGTGTTTTA
TGCCATGGCGCTGAAGATTGCGGACGCTATCTTGGAAGATTATGCTTCCA
TGCAGTGAggatcc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSSNGIEANLLTDPNDVAGRTVDYIIAGGGLTGLTTAARLTEDPNITVLVIESGSYESDRGAIIEDLNAYGDIFGSSVDHAYETVTLATNNQTALIRSGNGLGGSTLVNGGTWTRPHKAQVDSWETVFGNEGWNWDNVAAYSLQAERARAPNAKQIAAGHYFNASCHGVNGTVHAGPRDTGDDYSPIVQALMSAVEDRGVPTKKDFGCGDPHGVSMFPNTLHEDQVRSDAAREWLLPNYQRPNLQVLTGQFVGKVLLNQNTTTPRAVGVEFGTHKGNTHNVYAKHEVLLAAGSAISPTILEYSGIGMKSILEPLGIDTVVDLPVGLNLQDQTTATVRSRITSAGAGQGQAAWFATFNETFGDYAEKAHELLNTKLEQWAEEAVARGGFHNTTALLIQYENYRDWIVNHNVAYAELFLDTAGVASFDVWDLLPFTRGYVHILDKDPYLRHFAYDPQYFLNELDLLGQASALQLARNISNSGAMQTYFAGETIPGDNLAYDADLMAWAEYLPYHFRPNYHGVGTCSMMSREMGGVVDNAARVYGVQGLRVIDGSIPPTQVSSHVMTVFYAMALKIADAILEDYASMQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1202
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion