cDNA Clone RIPK2

• Plasmid: pEBG-2T-RICK/RIPK2
• Parent Plasmid: pEBG2T
• Expressed: GST-RIPK2
• Genbank: NM_003821
• Tag: GST
• Species: Human

Sequence of Insert

actagtATGAACGGGGAGGCCATCTGCAGCGCCCTGCCCACCATTCCCTACCACAAACTCGCCGACCTGCGCTACCTGAGCCGCGGCGCCTCTGGCACTGTGTCGTCCGCCCGCCACGCAGACTGGCGCGTCCAGGTGGCCGTGAAGCACCTGCACATCCACACTCCGCTGCTCGACAGTGAAAGAAAGGATGTCTTAAGAGAAGCTGAAATTTTACACAAAGCTAGATTTAGTTACATTCTTCCAATTTTGGGAATTTGCAATGAGCCTGAATTTTTGGGAATAGTTACTGAATACATGCCAAAT
GGATCATTAAATGAACTCCTACATAGGAAAACTGAATATCCTGATGTTGC
TTGGCCATTGAGATTTCGCATCCTGCATGAAATTGCCCTTGGTGTAAATT
ACCTGCACAATATGACTCCTCCTTTACTTCATCATGACTTGAAGACTCAG
AATATCTTATTGGACAATGAATTTCATGTTAAGATTGCAGATTTTGGTTT
ATCAAAGTGGCGCATGATGTCCCTCTCACAGTCACGAAGTAGCAAATCTG
CACCAGAAGGAGGGACAATTATCTATATGCCACCTGAAAACTATGAACCT
GGACAAAAATCAAGGGCCAGTATCAAGCACGATATATATAGCTATGCAGT
TATCACATGGGAAGTGTTATCCAGAAAACAGCCTTTTGAAGATGTCACCA
ATCCTTTGCAGATAATGTATAGTGTGTCACAAGGACATCGACCTGTTATT
AATGAAGAAAGTTTGCCATATGATATACCTCACCGAGCACGTATGATCTC
TCTAATAGAAAGTGGATGGGCACAAAATCCAGATGAAAGACCATCTTTCT
TAAAATGTTTAATAGAACTTGAACCAGTTTTGAGAACATTTGAAGAGATA
ACTTTTCTTGAAGCTGTTATTCAGCTAAAGAAAACAAAGTTACAGAGTGT
TTCAAGTGCCATTCACCTATGTGACAAGAAGAAAATGGAATTATCTCTGA
ACATACCTGTAAATCATGGTCCACAAGAGGAATCATGTGGATCCTCTCAG
CTCCATGAAAATAGTGGTTCTCCTGAAACTTCAAGGTCCCTGCCAGCTCC
TCAAGACAATGATTTTTTATCTAGAAAAGCTCAAGACTGTTATTTTATGA
AGCTGCATCACTGTCCTGGAAATCACAGTTGGGATAGCACCATTTCTGGA
TCTCAAAGGGCTGCATTCTGTGATCACAAGACCACTCCATGCTCTTCAGC
AATAATAAATCCACTCTCAACTGCAGGAAACTCAGAACGTCTGCAGCCTG
GTATAGCCCAGCAGTGGATCCAGAGCAAAAGGGAAGACATTGTGAACCAA
ATGACAGAAGCCTGCCTTAACCAGTCGCTAGATGCCCTTCTGTCCAGGGA
CTTGATCATGAAAGAGGACTATGAACTTGTTAGTACCAAGCCTACAAGGA
CCTCAAAAGTCAGACAATTACTAGACACTACTGACATCCAAGGAGAAGAA
TTTGCCAAAGTTATAGTACAAAAATTGAAAGATAACAAACAAATGGGTCT
TCAGCCTTACCCGGAAATACTTGTGGTTTCTAGATCACCATCTTTAAATT
TACTTCAAAATAAAAGCATGTAAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSTSMNGEAICSALPTIPYHKLADLR
YLSRGASGTVSSARHADWRVQVAVKHLHIHTPLLDSERKDVLREAEILHK
ARFSYILPILGICNEPEFLGIVTEYMPNGSLNELLHRKTEYPDVAWPLRF
RILHEIALGVNYLHNMTPPLLHHDLKTQNILLDNEFHVKIADFGLSKWRM
MSLSQSRSSKSAPEGGTIIYMPPENYEPGQKSRASIKHDIYSYAVITWEV
LSRKQPFEDVTNPLQIMYSVSQGHRPVINEESLPYDIPHRARMISLIESG
WAQNPDERPSFLKCLIELEPVLRTFEEITFLEAVIQLKKTKLQSVSSAIH
LCDKKKMELSLNIPVNHGPQEESCGSSQLHENSGSPETSRSLPAPQDNDF
LSRKAQDCYFMKLHHCPGNHSWDSTISGSQRAAFCDHKTTPCSSAIINPL
STAGNSERLQPGIAQQWIQSKREDIVNQMTEACLNQSLDALLSRDLIMKE
DYELVSTKPTRTSKVRQLLDTTDIQGEEFAKVIVQKLKDNKQMGLQPYPE
ILVVSRSPSLNLLQNKSM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU1181
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express RIPK2 with N-terminal GST ; Estimated Mol Weight 87619 Daltons