cDNA Clone beta-catenin

• Synonyms: CTNNB1
• Plasmid: pGEX6P-1-beta-catenin
• Parent Plasmid: pGEX6P1
• Expressed: GST-beta-catenin
• Genbank: NM_001904
• Tag: GST
• Species: Human

Sequence of Insert

ggatccGCTACTCAAGCTGATTTGATGGAGTTGGACATGGCCATGGAACC
AGACAGAAAAGCGGCTGTTAGTCACTGGCAGCAACAGTCTTACCTGGACT
CTGGAATCCATTCTGGTGCCACTACCACAGCTCCTTCTCTGAGTGGTAAA
GGCAATCCTGAGGAAGAGGATGTGGATACCTCCCAAGTCCTGTATGAGTG
GGAACAGGGATTTTCTCAGTCCTTCACTCAAGAACAAGTAGCTGATATTG
ATGGACAGTATGCAATGACTCGAGCTCAGAGGGTACGAGCTGCTATGTTC
CCTGAGACATTAGATGAGGGCATGCAGATCCCATCTACACAGTTTGATGC
TGCTCATCCCACTAATGTCCAGCGTTTGGCTGAACCATCACAGATGCTGA
AACATGCAGTTGTAAACTTGATTAACTATCAAGATGATGCAGAACTTGCC
ACACGTGCAATCCCTGAACTGACAAAACTGCTAAATGACGAGGACCAGGT
GGTGGTTAATAAGGCTGCAGTTATGGTCCATCAGCTTTCTAAAAAGGAAG
CTTCCAGACACGCTATCATGCGTTCTCCTCAGATGGTGTCTGCTATTGTA
CGTACCATGCAGAATACAAATGATGTAGAAACAGCTCGTTGTACCGCTGG
GACCTTGCATAACCTTTCCCATCATCGTGAGGGCTTACTGGCCATCTTTA
AGTCTGGAGGCATTCCTGCCCTGGTGAAAATGCTTGGTTCACCAGTGGAT
TCTGTGTTGTTTTATGCCATTACAACTCTCCACAACCTTTTATTACATCA
AGAAGGAGCTAAAATGGCAGTGCGTTTAGCTGGTGGGCTGCAGAAAATGG
TTGCCTTGCTCAACAAAACAAATGTTAAATTCTTGGCTATTACGACAGAC
TGCCTTCAAATTTTAGCTTATGGCAACCAAGAAAGCAAGCTCATCATACT
GGCTAGTGGTGGACCCCAAGCTTTAGTAAATATAATGAGGACCTATACTT
ACGAAAAACTACTGTGGACCACAAGCAGAGTGCTGAAGGTGCTATCTGTC
TGCTCTAGTAATAAGCCGGCTATTGTAGAAGCTGGTGGAATGCAAGCTTT
AGGACTTCACCTGACAGATCCAAGTCAACGTCTTGTTCAGAACTGTCTTT
GGACTCTCAGGAATCTTTCAGATGCTGCAACTAAACAGGAAGGGATGGAA
GGTCTCCTTGGGACTCTTGTTCAGCTTCTGGGTTCAGATGATATAAATGT
GGTCACCTGTGCAGCTGGAATTCTTTCTAACCTCACTTGCAATAATTATA
AGAACAAGATGATGGTCTGCCAAGTGGGTGGTATAGAGGCTCTTGTGCGT
ACTGTCCTTCGGGCTGGTGACAGGGAAGACATCACTGAGCCTGCCATCTG
TGCTCTTCGTCATCTGACCAGCCGACACCAAGAAGCAGAGATGGCCCAGA
ATGCAGTTCGCCTTCACTATGGACTACCAGTTGTGGTTAAGCTCTTACAC
CCACCATCCCACTGGCCTCTGATAAAGGCTACTGTTGGATTGATTCGAAA
TCTTGCCCTTTGTCCCGCAAATCATGCACCTTTGCGTGAGCAGGGTGCCA
TTCCACGACTAGTTCAGTTGCTTGTTCGTGCACATCAGGATACCCAGCGC
CGTACGTCCATGGGTGGGACACAGCAGCAATTTGTGGAGGGGGTCCGCAT
GGAAGAAATAGTTGAAGGTTGTACCGGAGCCCTTCACATCCTAGCTCGGG
ATGTTCACAACCGAATTGTTATCAGAGGACTAAATACCATTCCATTGTTT
GTGCAGCTGCTTTATTCTCCCATTGAAAACATCCAAAGAGTAGCTGCAGG
GGTCCTCTGTGAACTTGCTCAGGACAAGGAAGCTGCAGAAGCTATTGAAG
CTGAGGGAGCCACAGCTCCTCTGACAGAGTTACTTCACTCTAGGAATGAA
GGTGTGGCGACATATGCAGCTGCTGTTTTGTTCCGAATGTCTGAGGACAA
GCCACAAGATTACAAGAAACGGCTTTCAGTTGAGCTGACCAGCTCTCTCT
TCAGAACAGAGCCAATGGCTTGGAATGAGACTGCTGATCTTGGACTTGAT
ATTGGTGCCCAGGGAGAACCCCTTGGATATCGCCAGGATGATCCTAGCTA
TCGTTCTTTTCACTCTGGTGGATATGGCCAGGATGCCTTGGGTATGGACC
CCATGATGGAACATGAGATGGGTGGCCACCACCCTGGTGCTGACTATCCA
GTTGATGGGCTGCCAGATCTGGGGCATGCCCAGGACCTCATGGATGGGCT
GCCTCCAGGTGACAGCAATCAGCTGGCCTGGTTTGATACTGACCTGTAAc
ccggg

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1105
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion