cDNA Clone MELK

• Plasmid: pCMV5-HA MELK
• Parent Plasmid: pCMV5
• Expressed: HA-MELK
• Genbank: NM_014791
• Tag: HA
• Species: Human

Sequence of Insert

gaattcGCCACCATGTACCCATACGATGTGCCAGATTACGCCAAAGATTA
TGATGAACTTCTCAAATATTATGAATTACATGAAACTATTGGGACAGGTG
GCTTTGCAAAGGTCAAACTTGCCTGCCATATCCTTACTGGAGAGATGGTA
GCTATAAAAATCATGGATAAAAACACACTAGGGAGTGATTTGCCCCGGAT
CAAAACGGAGATTGAGGCCTTGAAGAACCTGAGACATCAGCATATATGTC
AACTCTACCATGTGCTAGAGACAGCCAACAAAATATTCATGGTTCTTGAG
TACTGCCCTGGAGGAGAGCTGTTTGACTATATAATTTCCCAGGATCGCCT
GTCAGAAGAGGAGACCCGGGTTGTCTTCCGTCAGATAGTATCTGCTGTTG
CTTATGTGCACAGCCAGGGCTATGCTCACAGGGACCTCAAGCCAGAAAAT
TTGCTGTTTGATGAATATCATAAATTAAAGCTGATTGACTTTGGTCTCTG
TGCAAAACCCAAGGGTAACAAGGATTACCATCTACAGACATGCTGTGGGA
GTCTGGCTTATGCAGCACCTGAGTTAATACAAGGCAAATCATATCTTGGA
TCAGAGGCAGATGTTTGGAGCATGGGCATACTGTTATATGTTCTTATGTG
TGGATTTCTACCATTTGATGATGATAATGTAATGGCTTTATACAAGAAGA
TTATGAGAGGAAAATATGATGTTCCCAAGTGGCTCTCTCCCAGTAGCATT
CTGCTTCTTCAACAAATGCTGCAGGTGGACCCAAAGAAACGGATTTCTAT
GAAAAATCTATTGAACCATCCCTGGATCATGCAAGATTACAACTATCCTG
TTGAGTGGCAAAGCAAGAATCCTTTTATTCACCTCGATGATGATTGCGTA
ACAGAACTTTCTGTACATCACAGAAACAACAGGCAAACAATGGAGGATTT
AATTTCACTGTGGCAGTATGATCACCTCACGGCTACCTATCTTCTGCTTC
TAGCCAAGAAGGCTCGGGGAAAACCAGTTCGTTTAAGGCTTTCTTCTTTC
TCCTGTGGACAAGCCAGTGCTACCCCATTCACAGACATCAAGTCAAATAA
TTGGAGTCTGGAAGATGTGACCGCAAGTGATAAAAATTATGTGGCGGGAT
TAATAGACTATGATTGGTGTGAAGATGATTTATCAACAGGTGCTGCTACT
CCCCGAACATCACAGTTTACCAAGTACTGGACAGAATCAAATGGGGTGGA
ATCTAAATCATTAACTCCAGCCTTATGCAGAACACCTGCAAATAAATTAA
AGAACAAAGAAAATGTATATACTCCTAAGTCTGCTGTAAAGAATGAAGAG
TACTTTATGTTTCCTGAGCCAAAGACTCCAGTTAATAAGAACCAGCATAA
GAGAGAAATACTCACTACGCCAAATCGTTACACTACACCCTCAAAAGCTA
GAAACCAGTGCCTGAAAGAAACTCCAATTAAAATACCAGTAAATTCAACA
GGAACAGACAAGTTAATGACAGGTGTCATTAGCCCTGAGAGGCGGTGCCG
CTCAGTGGAATTGGATCTCAACCAAGCACATATGGAGGAGACTCCAAAAA
GAAAGGGAGCCAAAGTGTTTGGGAGCCTTGAAAGGGGGTTGGATAAGGTT
ATCACTGTGCTCACCAGGAGCAAAAGGAAGGGTTCTGCCAGAGACGGGCC
CAGAAGACTAAAGCTTCACTATAATGTGACTACAACTAGATTAGTGAATC
CAGATCAACTGTTGAATGAAATAATGTCTATTCTTCCAAAGAAGCATGTT
GACTTTGTACAAAAGGGTTATACACTGAAGTGTCAAACACAGTCAGATTT
TGGGAAAGTGACAATGCAATTTGAATTAGAAGTGTGCCAGCTTCAAAAAC
CCGATGTGGTGGGTATCAGGAGGCAGCGGCTTAAGGGCGATGCCTGGGTT
TACAAAAGATTAGTGGAAGACATCCTATCTAGCTGCAAGGTATAAggatc
c

Amino Acid Sequence

MYPYDVPDYAKDYDELLKYYELHETIGTGGFAKVKLACHILTGEMVAIKI
MDKNTLGSDLPRIKTEIEALKNLRHQHICQLYHVLETANKIFMVLEYCPG
GELFDYIISQDRLSEEETRVVFRQIVSAVAYVHSQGYAHRDLKPENLLFD
EYHKLKLIDFGLCAKPKGNKDYHLQTCCGSLAYAAPELIQGKSYLGSEAD
VWSMGILLYVLMCGFLPFDDDNVMALYKKIMRGKYDVPKWLSPSSILLLQ
QMLQVDPKKRISMKNLLNHPWIMQDYNYPVEWQSKNPFIHLDDDCVTELS
VHHRNNRQTMEDLISLWQYDHLTATYLLLLAKKARGKPVRLRLSSFSCGQ
ASATPFTDIKSNNWSLEDVTASDKNYVAGLIDYDWCEDDLSTGAATPRTS
QFTKYWTESNGVESKSLTPALCRTPANKLKNKENVYTPKSAVKNEEYFMF
PEPKTPVNKNQHKREILTTPNRYTTPSKARNQCLKETPIKIPVNSTGTDK
LMTGVISPERRCRSVELDLNQAHMEETPKRKGAKVFGSLERGLDKVITVL
TRSKRKGSARDGPRRLKLHYNVTTTRLVNPDQLLNEIMSILPKKHVDFVQ
KGYTLKCQTQSDFGKVTMQFELEVCQLQKPDVVGIRRQRLKGDAWVYKRL
VEDILSSCKV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU1102
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express MELK with N terminal HA tag