cDNA Clone SMARCA3

• Plasmid: pcDNA5-FRT/TO-FLAG SMARCA3
• Parent Plasmid: pcDNA5D FRT/TO FLAG
• Expressed: FLAG SMARCA3
• Genbank: NM_003071.3*
• Tag: FLAG
• Species: Human

Sequence of Insert

AGATCTATGTCCTGGATGTTCAAGAGGGATCCAGTTTGGAAGTACTTGCAGACTGTCCAGTATGGAGTTCATGGAAATTTTCCACGCCTCTCATATCCAACTTTCTTTCCACGTTTTGAATTCCAAGATGTTATCCCTCCAGATGACTTTCTAACTAGTGATGAAGAAGTAGATTCCGTTTTATTTGGAAGTTTGAGAGGTCATGTGGTTGGACTACGCTATTACACGGGAGTAGTTAATAATAATGAAATGGTTGCATTACAACGAGATCCTAATAACCCTTATGATAAGAATGCAATTAAAGTA
AACAATGTGAATGGAAATCAAGTTGGCCATTTAAAGAAAGAGCTTGCAGG
TGCTTTGGCCTATATCATGGACAACAAATTGGCACAAATTGAAGGGGTAG
TTCCTTTTGGTGCAAACAATGCTTTTACCATGCCTCTGCATATGACTTTT
TGGGGAAAAGAAGAAAATAGAAAAGCGGTTTCAGATCAGTTGAAGAAACA
TGGATTTAAATTGGGTCCTGCACCAAAAACTTTAGGATTCAATTTGGAAA
GTGGTTGGGGCTCTGGAAGAGCTGGACCAAGCTATAGTATGCCAGTGCAT
GCTGCAGTACAGATGACAACTGAACAGCTTAAAACAGAATTTGACAAATT
GTTTGAAGATTTAAAAGAAGATGATAAAACCCATGAAATGGAACCAGCTG
AGGCTATTGAAACACCACTGCTTCCACATCAAAAACAAGCTCTAGCTTGG
ATGGTGTCACGGGAAAATAGCAAAGAACTTCCACCATTCTGGGAACAGCG
AAATGACTTATACTATAACACAATAACAAATTTTTCTGAGAAGGACCGAC
CAGAAAATGTCCATGGAGGAATTTTAGCTGATGATATGGGTTTGGGTAAA
ACTCTTACAGCCATTGCAGTAATCCTTACCAACTTCCATGATGGCAGACC
TCTTCCTATTGAAAGAGTTAAAAAGAATCTACTGAAGAAGGAATATAATG
TTAACGATGACTCTATGAAACTTGGAGGAAACAATACCAGTGAAAAGGCA
GATGGACTAAGCAAAGACGCATCTAGATGTAGTGAACAACCCAGTATTTC
AGATATCAAGGAGAAGAGTAAGTTTCGCATGTCAGAATTGTCTAGCTCCC
GCCCCAAAAGAAGAAAAACTGCTGTCCAGTACATAGAAAGCAGTGATTCA
GAGGAAATTGAAACAAGTGAATTGCCGCAGAAAATGAAAGGCAAACTGAA
AAATGTACAGTCTGAAACTAAAGGCAGGGCGAAAGGATCTTCTAAGGTTA
TAGAAGATGTGGCATTTGCATGTGCATTAACTTCATCTGTTCCTACAACA
AAAAAGAAAATGTTGAAAAAGGGAGCTTGTGCAGTGGAGGGGTCAAAGAA
AACTGATGTTGAGGAGAGACCAAGAACAACACTGATCATCTGTCCGCTTT
CTGTGTTAAGCAACTGGATTGACCAGTTTGGACAACATATAAAATCAGAT
GTACACTTGAATTTTTATGTTTATTATGGTCCTGATCGTATTAGAGAACC
GGCCTTACTTTCAAAACAGGATATTGTTTTGACTACGTATAATATTTTAA
CTCATGACTATGGAACTAAAGGAGATAGTCCATTACATAGCATAAGGTGG
CTAAGAGTGATCCTGGATGAAGGACATGCCATACGAAATCCAAATGCTCA
GCAGACAAAAGCTGTACTTGACTTAGAATCAGAAAGAAGATGGGTTTTGA
CAGGTACTCCAATCCAGAATTCTTTAAAGGACTTGTGGTCTCTTCTTTCC
TTTTTAAAACTTAAACCATTTATTGATAGAGAATGGTGGCATAGAACAAT
ACAGCGTCCTGTCACAATGGGAGATGAAGGAGGACTTAGGCGTTTACAGT
CCCTAATTAAAAATATTACACTTAGAAGAACAAAGACAAGCAAAATTAAA
GGAAAACCTGTTTTGGAGTTACCAGAACGTAAAGTATTTATTCAGCACAT
TACACTTTCAGATGAAGAGAGAAAGATTTATCAGTCTGTGAAAAATGAAG
GCAGAGCCACTATTGGAAGGTATTTTAATGAAGGGACTGTCCTGGCACAT
TATGCAGATGTCCTGGGTCTTTTGCTTAGACTGCGGCAAATTTGTTGCCA
TACTTACCTTCTTACAAATGCAGTGTCTTCCAATGGCCCCTCAGGAAATG
ATACACCTGAAGAACTGAGAAAGAAGTTAATAAGGAAGATGAAGTTAATT
CTGAGCTCAGGTTCAGATGAGGAATGTGCAATTTGCCTGGATTCTTTAAC
AGTTCCTGTGATAACACATTGTGCACATGTATTTTGTAAACCCTGTATTT
GCCAAGTCATTCAGAATGAGCAGCCACATGCTAAATGCCCTTTATGCAGA
AATGATATACATGAAGATAATTTATTAGAATGTCCTCCAGAAGAATTAGC
ACGTGACAGTGAGAAAAAGTCTGATATGGAATGGACATCCAGTTCAAAGA
TTAATGCGCTAATGCACGCATTGACTGACTTAAGAAAGAAGAATCCCAAC
ATAAAAAGTTTGGTTGTTTCTCAGTTTACAACATTCCTGTCTTTAATAGA
AATACCACTTAAAGCCTCTGGATTTGTGTTTACTCGTTTGGATGGTTCCA
TGGCCCAAAAGAAAAGAGTTGAATCAATTCAGTGTTTTCAAAACACTGAA
GCAGGATCTCCAACTATAATGCTTCTGTCCTTAAAAGCAGGTGGAGTTGG
TTTGAATCTGTCTGCAGCTTCTCGAGTGTTTTTAATGGATCCAGCCTGGA
ATCCTGCTGCTGAAGATCAGTGCTTTGACAGATGCCATAGACTTGGTCAG
AAGCAAGAAGTTATCATCACAAAATTCATTGTAAAGGACTCTGTTGAAGA
AAATATGCTGAAAATACAAAACAAAAAGAGAGAACTTGCAGCAGGAGCCT
TTGGAACTAAAAAACCAAATGCTGACGAAATGAAACAAGCCAAAATTAAT
GAAATCAGAACATTAATTGACTTATAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMSWMFKRDPVWKYLQTVQYGVHGNFPRLSYPTFFPRFEF
QDVIPPDDFLTSDEEVDSVLFGSLRGHVVGLRYYTGVVNNNEMVALQRDP
NNPYDKNAIKVNNVNGNQVGHLKKELAGALAYIMDNKLAQIEGVVPFGAN
NAFTMPLHMTFWGKEENRKAVSDQLKKHGFKLGPAPKTLGFNLESGWGSG
RAGPSYSMPVHAAVQMTTEQLKTEFDKLFEDLKEDDKTHEMEPAEAIETP
LLPHQKQALAWMVSRENSKELPPFWEQRNDLYYNTITNFSEKDRPENVHG
GILADDMGLGKTLTAIAVILTNFHDGRPLPIERVKKNLLKKEYNVNDDSM
KLGGNNTSEKADGLSKDASRCSEQPSISDIKEKSKFRMSELSSSRPKRRK
TAVQYIESSDSEEIETSELPQKMKGKLKNVQSETKGRAKGSSKVIEDVAF
ACALTSSVPTTKKKMLKKGACAVEGSKKTDVEERPRTTLIICPLSVLSNW
IDQFGQHIKSDVHLNFYVYYGPDRIREPALLSKQDIVLTTYNILTHDYGT
KGDSPLHSIRWLRVILDEGHAIRNPNAQQTKAVLDLESERRWVLTGTPIQ
NSLKDLWSLLSFLKLKPFIDREWWHRTIQRPVTMGDEGGLRRLQSLIKNI
TLRRTKTSKIKGKPVLELPERKVFIQHITLSDEERKIYQSVKNEGRATIG
RYFNEGTVLAHYADVLGLLLRLRQICCHTYLLTNAVSSNGPSGNDTPEEL
RKKLIRKMKLILSSGSDEECAICLDSLTVPVITHCAHVFCKPCICQVIQN
EQPHAKCPLCRNDIHEDNLLECPPEELARDSEKKSDMEWTSSSKINALMH
ALTDLRKKNPNIKSLVVSQFTTFLSLIEIPLKASGFVFTRLDGSMAQKKR
VESIQCFQNTEAGSPTIMLLSLKAGGVGLNLSAASRVFLMDPAWNPAAED
QCFDRCHRLGQKQEVIITKFIVKDSVEENMLKIQNKKRELAAGAFGTKKP
NADEMKQAKINEIRTLIDL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11613
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence differences compared with NM_003071.3 CDS:; g909a (sil T303) ; delta agc nt1284-1286 (delta residue A429*) ; *derives from EST -also deleted in some published seqs.; Grow at or below 30ºC
• Low Temp: Yes