cDNA Clone SMARCA3

• Plasmid: pGFPTAP BN SMARCA3
• Parent Plasmid: pGFP TAP BN
• Expressed: GFP-TAP SMARCA3
• Genbank: NM_003071.3
• Tag: GFP
• Tag: TAP
• Species: Human

Sequence of Insert

GCGGCCGCCATGTCCTGGATGTTCAAGAGGGATCCAGTTTGGAAGTACTT
GCAGACTGTCCAGTATGGAGTTCATGGAAATTTTCCACGCCTCTCATATC
CAACTTTCTTTCCACGTTTTGAATTCCAAGATGTTATCCCTCCAGATGAC
TTTCTAACTAGTGATGAAGAAGTAGATTCCGTTTTATTTGGAAGTTTGAG
AGGTCATGTGGTTGGACTACGCTATTACACGGGAGTAGTTAATAATAATG
AAATGGTTGCATTACAACGAGATCCTAATAACCCTTATGATAAGAATGCA
ATTAAAGTAAACAATGTGAATGGAAATCAAGTTGGCCATTTAAAGAAAGA
GCTTGCAGGTGCTTTGGCCTATATCATGGACAACAAATTGGCACAAATTG
AAGGGGTAGTTCCTTTTGGTGCAAACAATGCTTTTACCATGCCTCTGCAT
ATGACTTTTTGGGGAAAAGAAGAAAATAGAAAAGCGGTTTCAGATCAGTT
GAAGAAACATGGATTTAAATTGGGTCCTGCACCAAAAACTTTAGGATTCA
ATTTGGAAAGTGGTTGGGGCTCTGGAAGAGCTGGACCAAGCTATAGTATG
CCAGTGCATGCTGCAGTACAGATGACAACTGAACAGCTTAAAACAGAATT
TGACAAATTGTTTGAAGATTTAAAAGAAGATGATAAAACCCATGAAATGG
AACCAGCTGAGGCTATTGAAACACCACTGCTTCCACATCAAAAACAAGCT
CTAGCTTGGATGGTGTCACGGGAAAATAGCAAAGAACTTCCACCATTCTG
GGAACAGCGAAATGACTTATACTATAACACAATAACAAATTTTTCTGAGA
AGGACCGACCAGAAAATGTCCATGGAGGAATTTTAGCTGATGATATGGGT
TTGGGTAAAACTCTTACAGCCATTGCAGTAATCCTTACCAACTTCCATGA
TGGCAGACCTCTTCCTATTGAAAGAGTTAAAAAGAATCTACTGAAGAAGG
AATATAATGTTAACGATGACTCTATGAAACTTGGAGGAAACAATACCAGT
GAAAAGGCAGATGGACTAAGCAAAGACGCATCTAGATGTAGTGAACAACC
CAGTATTTCAGATATCAAGGAGAAGAGTAAGTTTCGCATGTCAGAATTGT
CTAGCTCCCGCCCCAAAAGAAGAAAAACTGCTGTCCAGTACATAGAAAGC
AGTGATTCAGAGGAAATTGAAACAAGTGAATTGCCGCAGAAAATGAAAGG
CAAACTGAAAAATGTACAGTCTGAAACTAAAGGCAGGGCGAAAGGATCTT
CTAAGGTTATAGAAGATGTGGCATTTGCATGTGCATTAACTTCATCTGTT
CCTACAACAAAAAAGAAAATGTTGAAAAAGGGAGCTTGTGCAGTGGAGGG
GTCAAAGAAAACTGATGTTGAGGAGAGACCAAGAACAACACTGATCATCT
GTCCGCTTTCTGTGTTAAGCAACTGGATTGACCAGTTTGGACAACATATA
AAATCAGATGTACACTTGAATTTTTATGTTTATTATGGTCCTGATCGTAT
TAGAGAACCGGCCTTACTTTCAAAACAGGATATTGTTTTGACTACGTATA
ATATTTTAACTCATGACTATGGAACTAAAGGAGATAGTCCATTACATAGC
ATAAGGTGGCTAAGAGTGATCCTGGATGAAGGACATGCCATACGAAATCC
AAATGCTCAGCAGACAAAAGCTGTACTTGACTTAGAATCAGAAAGAAGAT
GGGTTTTGACAGGTACTCCAATCCAGAATTCTTTAAAGGACTTGTGGTCT
CTTCTTTCCTTTTTAAAACTTAAACCATTTATTGATAGAGAATGGTGGCA
TAGAACAATACAGCGTCCTGTCACAATGGGAGATGAAGGAGGACTTAGGC
GTTTACAGTCCCTAATTAAAAATATTACACTTAGAAGAACAAAGACAAGC
AAAATTAAAGGAAAACCTGTTTTGGAGTTACCAGAACGTAAAGTATTTAT
TCAGCACATTACACTTTCAGATGAAGAGAGAAAGATTTATCAGTCTGTGA
AAAATGAAGGCAGAGCCACTATTGGAAGGTATTTTAATGAAGGGACTGTC
CTGGCACATTATGCAGATGTCCTGGGTCTTTTGCTTAGACTGCGGCAAAT
TTGTTGCCATACTTACCTTCTTACAAATGCAGTGTCTTCCAATGGCCCCT
CAGGAAATGATACACCTGAAGAACTGAGAAAGAAGTTAATAAGGAAGATG
AAGTTAATTCTGAGCTCAGGTTCAGATGAGGAATGTGCAATTTGCCTGGA
TTCTTTAACAGTTCCTGTGATAACACATTGTGCACATGTATTTTGTAAAC
CCTGTATTTGCCAAGTCATTCAGAATGAGCAGCCACATGCTAAATGCCCT
TTATGCAGAAATGATATACATGAAGATAATTTATTAGAATGTCCTCCAGA
AGAATTAGCACGTGACAGTGAGAAAAAGTCTGATATGGAATGGACATCCA
GTTCAAAGATTAATGCGCTAATGCACGCATTGACTGACTTAAGAAAGAAG
AATCCCAACATAAAAAGTTTGGTTGTTTCTCAGTTTACAACATTCCTGTC
TTTAATAGAAATACCACTTAAAGCCTCTGGATTTGTGTTTACTCGTTTGG
ATGGTTCCATGGCCCAAAAGAAAAGAGTTGAATCAATTCAGTGTTTTCAA
AACACTGAAGCAGGATCTCCAACTATAATGCTTCTGTCCTTAAAAGCAGG
TGGAGTTGGTTTGAATCTGTCTGCAGCTTCTCGAGTGTTTTTAATGGATC
CAGCCTGGAATCCTGCTGCTGAAGATCAGTGCTTTGACAGATGCCATAGA
CTTGGTCAGAAGCAAGAAGTTATCATCACAAAATTCATTGTAAAGGACTC
TGTTGAAGAAAATATGCTGAAAATACAAAACAAAAAGAGAGAACTTGCAG
CAGGAGCCTTTGGAACTAAAAAACCAAATGCTGACGAAATGAAACAAGCC
AAAATTAATGAAATCAGAACATTAATTGACTTATAAGTCGAC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSFDIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGRSMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSRSGTAAAMSWMFKRDPVWKYLQTVQYGVHGNFPRLSYPTFFPRFEFQDVIPPDDFLTSDEEVDSVLFGSLRGHVVGLRYYTGVVNNNEMVALQRDPNNPYDKNAIKVNNVNGNQVGHLKKELAGALAYIMDNKLAQIEGVVPFGANNAFTMPLHMTFWGKEENRKAVSDQLKKHGFKLGPAPKTLGFNLESGWGSGRAGPSYSMPVHAAVQMTTEQLKTEFDKLFEDLKEDDKTHEMEPAEAIETPLLPHQKQALAWMVSRENSKELPPFWEQRNDLYYNTITNFSEKDRPENVHGGILADDMGLGKTLTAIAVILTNFHDGRPLPIERVKKNLLKKEYNVNDDSMKLGGNNTSEKADGLSKDASRCSEQPSISDIKEKSKFRMSELSSSRPKRRKTAVQYIESSDSEEIETSELPQKMKGKLKNVQSETKGRAKGSSKVIEDVAFACALTSSVPTTKKKMLKKGACAVEGSKKTDVEERPRTTLIICPLSVLSNWIDQFGQHIKSDVHLNFYVYYGPDRIREPALLSKQDIVLTTYNILTHDYGTKGDSPLHSIRWLRVILDEGHAIRNPNAQQTKAVLDLESERRWVLTGTPIQNSLKDLWSLLSFLKLKPFIDREWWHRTIQRPVTMGDEGGLRRLQSLIKNITLRRTKTSKIKGKPVLELPERKVFIQHITLSDEERKIYQSVKNEGRATIGRYFNEGTVLAHYADVLGLLLRLRQICCHTYLLTNAVSSNGPSGNDTPEELRKKLIRKMKLILSSGSDEECAICLDSLTVPVITHCAHVFCKPCICQVIQNEQPHAKCPLCRNDIHEDNLLECPPEELARDSEKKSDMEWTSSSKINALMHALTDLRKKNPNIKSLVVSQFTTFLSLIEIPLKASGFVFTRLDGSMAQKKRVESIQCFQNTEAGSPTIMLLSLKAGGVGLNLSAASRVFLMDPAWNPAAEDQCFDRCHRLGQKQEVIITKFIVKDSVEENMLKIQNKKRELAAGAFGTKKPNADEMKQAKINEIRTLIDL*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU11497
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence differences compared with NM_003071.3 CDS:; g909a (sil T303) ; delta agc nt1284-1286 (delta residue A429*) ; *derives from EST - also deleted in some published seqs.; Grow at or below 30ºC
• Low Temp: Yes