cDNA Clone SMARCA3

• Plasmid: pFastBacHTc SMARCA3
• Parent Plasmid: pFastbac HTc
• Expressed: 6His-SMARCA3
• Genbank: NM_003071.3
• Tag: 6His
• Species: Human

Sequence of Insert

GTCGACATGTCCTGGATGTTCAAGAGGGATCCAGTTTGGAAGTACTTGCA
GACTGTCCAGTATGGAGTTCATGGAAATTTTCCACGCCTCTCATATCCAA
CTTTCTTTCCACGTTTTGAATTCCAAGATGTTATCCCTCCAGATGACTTT
CTAACTAGTGATGAAGAAGTAGATTCCGTTTTATTTGGAAGTTTGAGAGG
TCATGTGGTTGGACTACGCTATTACACGGGAGTAGTTAATAATAATGAAA
TGGTTGCATTACAACGAGATCCTAATAACCCTTATGATAAGAATGCAATT
AAAGTAAACAATGTGAATGGAAATCAAGTTGGCCATTTAAAGAAAGAGCT
TGCAGGTGCTTTGGCCTATATCATGGACAACAAATTGGCACAAATTGAAG
GGGTAGTTCCTTTTGGTGCAAACAATGCTTTTACCATGCCTCTGCATATG
ACTTTTTGGGGAAAAGAAGAAAATAGAAAAGCGGTTTCAGATCAGTTGAA
GAAACATGGATTTAAATTGGGTCCTGCACCAAAAACTTTAGGATTCAATT
TGGAAAGTGGTTGGGGCTCTGGAAGAGCTGGACCAAGCTATAGTATGCCA
GTGCATGCTGCAGTACAGATGACAACTGAACAGCTTAAAACAGAATTTGA
CAAATTGTTTGAAGATTTAAAAGAAGATGATAAAACCCATGAAATGGAAC
CAGCTGAGGCTATTGAAACACCACTGCTTCCACATCAAAAACAAGCTCTA
GCTTGGATGGTGTCACGGGAAAATAGCAAAGAACTTCCACCATTCTGGGA
ACAGCGAAATGACTTATACTATAACACAATAACAAATTTTTCTGAGAAGG
ACCGACCAGAAAATGTCCATGGAGGAATTTTAGCTGATGATATGGGTTTG
GGTAAAACTCTTACAGCCATTGCAGTAATCCTTACCAACTTCCATGATGG
CAGACCTCTTCCTATTGAAAGAGTTAAAAAGAATCTACTGAAGAAGGAAT
ATAATGTTAACGATGACTCTATGAAACTTGGAGGAAACAATACCAGTGAA
AAGGCAGATGGACTAAGCAAAGACGCATCTAGATGTAGTGAACAACCCAG
TATTTCAGATATCAAGGAGAAGAGTAAGTTTCGCATGTCAGAATTGTCTA
GCTCCCGCCCCAAAAGAAGAAAAACTGCTGTCCAGTACATAGAAAGCAGT
GATTCAGAGGAAATTGAAACAAGTGAATTGCCGCAGAAAATGAAAGGCAA
ACTGAAAAATGTACAGTCTGAAACTAAAGGCAGGGCGAAAGGATCTTCTA
AGGTTATAGAAGATGTGGCATTTGCATGTGCATTAACTTCATCTGTTCCT
ACAACAAAAAAGAAAATGTTGAAAAAGGGAGCTTGTGCAGTGGAGGGGTC
AAAGAAAACTGATGTTGAGGAGAGACCAAGAACAACACTGATCATCTGTC
CGCTTTCTGTGTTAAGCAACTGGATTGACCAGTTTGGACAACATATAAAA
TCAGATGTACACTTGAATTTTTATGTTTATTATGGTCCTGATCGTATTAG
AGAACCGGCCTTACTTTCAAAACAGGATATTGTTTTGACTACGTATAATA
TTTTAACTCATGACTATGGAACTAAAGGAGATAGTCCATTACATAGCATA
AGGTGGCTAAGAGTGATCCTGGATGAAGGACATGCCATACGAAATCCAAA
TGCTCAGCAGACAAAAGCTGTACTTGACTTAGAATCAGAAAGAAGATGGG
TTTTGACAGGTACTCCAATCCAGAATTCTTTAAAGGACTTGTGGTCTCTT
CTTTCCTTTTTAAAACTTAAACCATTTATTGATAGAGAATGGTGGCATAG
AACAATACAGCGTCCTGTCACAATGGGAGATGAAGGAGGACTTAGGCGTT
TACAGTCCCTAATTAAAAATATTACACTTAGAAGAACAAAGACAAGCAAA
ATTAAAGGAAAACCTGTTTTGGAGTTACCAGAACGTAAAGTATTTATTCA
GCACATTACACTTTCAGATGAAGAGAGAAAGATTTATCAGTCTGTGAAAA
ATGAAGGCAGAGCCACTATTGGAAGGTATTTTAATGAAGGGACTGTCCTG
GCACATTATGCAGATGTCCTGGGTCTTTTGCTTAGACTGCGGCAAATTTG
TTGCCATACTTACCTTCTTACAAATGCAGTGTCTTCCAATGGCCCCTCAG
GAAATGATACACCTGAAGAACTGAGAAAGAAGTTAATAAGGAAGATGAAG
TTAATTCTGAGCTCAGGTTCAGATGAGGAATGTGCAATTTGCCTGGATTC
TTTAACAGTTCCTGTGATAACACATTGTGCACATGTATTTTGTAAACCCT
GTATTTGCCAAGTCATTCAGAATGAGCAGCCACATGCTAAATGCCCTTTA
TGCAGAAATGATATACATGAAGATAATTTATTAGAATGTCCTCCAGAAGA
ATTAGCACGTGACAGTGAGAAAAAGTCTGATATGGAATGGACATCCAGTT
CAAAGATTAATGCGCTAATGCACGCATTGACTGACTTAAGAAAGAAGAAT
CCCAACATAAAAAGTTTGGTTGTTTCTCAGTTTACAACATTCCTGTCTTT
AATAGAAATACCACTTAAAGCCTCTGGATTTGTGTTTACTCGTTTGGATG
GTTCCATGGCCCAAAAGAAAAGAGTTGAATCAATTCAGTGTTTTCAAAAC
ACTGAAGCAGGATCTCCAACTATAATGCTTCTGTCCTTAAAAGCAGGTGG
AGTTGGTTTGAATCTGTCTGCAGCTTCTCGAGTGTTTTTAATGGATCCAG
CCTGGAATCCTGCTGCTGAAGATCAGTGCTTTGACAGATGCCATAGACTT
GGTCAGAAGCAAGAAGTTATCATCACAAAATTCATTGTAAAGGACTCTGT
TGAAGAAAATATGCTGAAAATACAAAACAAAAAGAGAGAACTTGCAGCAG
GAGCCTTTGGAACTAAAAAACCAAATGCTGACGAAATGAAACAAGCCAAA
ATTAATGAAATCAGAACATTAATTGACTTATAAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGIRNSKAYVDMSWMFKRDPVWKYL
QTVQYGVHGNFPRLSYPTFFPRFEFQDVIPPDDFLTSDEEVDSVLFGSLR
GHVVGLRYYTGVVNNNEMVALQRDPNNPYDKNAIKVNNVNGNQVGHLKKE
LAGALAYIMDNKLAQIEGVVPFGANNAFTMPLHMTFWGKEENRKAVSDQL
KKHGFKLGPAPKTLGFNLESGWGSGRAGPSYSMPVHAAVQMTTEQLKTEF
DKLFEDLKEDDKTHEMEPAEAIETPLLPHQKQALAWMVSRENSKELPPFW
EQRNDLYYNTITNFSEKDRPENVHGGILADDMGLGKTLTAIAVILTNFHD
GRPLPIERVKKNLLKKEYNVNDDSMKLGGNNTSEKADGLSKDASRCSEQP
SISDIKEKSKFRMSELSSSRPKRRKTAVQYIESSDSEEIETSELPQKMKG
KLKNVQSETKGRAKGSSKVIEDVAFACALTSSVPTTKKKMLKKGACAVEG
SKKTDVEERPRTTLIICPLSVLSNWIDQFGQHIKSDVHLNFYVYYGPDRI
REPALLSKQDIVLTTYNILTHDYGTKGDSPLHSIRWLRVILDEGHAIRNP
NAQQTKAVLDLESERRWVLTGTPIQNSLKDLWSLLSFLKLKPFIDREWWH
RTIQRPVTMGDEGGLRRLQSLIKNITLRRTKTSKIKGKPVLELPERKVFI
QHITLSDEERKIYQSVKNEGRATIGRYFNEGTVLAHYADVLGLLLRLRQI
CCHTYLLTNAVSSNGPSGNDTPEELRKKLIRKMKLILSSGSDEECAICLD
SLTVPVITHCAHVFCKPCICQVIQNEQPHAKCPLCRNDIHEDNLLECPPE
ELARDSEKKSDMEWTSSSKINALMHALTDLRKKNPNIKSLVVSQFTTFLS
LIEIPLKASGFVFTRLDGSMAQKKRVESIQCFQNTEAGSPTIMLLSLKAG
GVGLNLSAASRVFLMDPAWNPAAEDQCFDRCHRLGQKQEVIITKFIVKDS
VEENMLKIQNKKRELAAGAFGTKKPNADEMKQAKINEIRTLIDL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU11484
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; Sequence differences compared with NM_003071.3 CDS:; g909a (sil T303) ; delta agc nt1284-1286 (delta residue A429*) ; *derives from EST -also deleted in some published seqs.; ; Estimated MW of expressed protein : 118186 Daltons Grow at or below 30ºC
• Low Temp: Yes