cDNA Clone SMARCA3

• Plasmid: pGEX6P-3-SMARCA3 H200-end
• Parent Plasmid: pGEX6P3
• Expressed: GST-SMARCA3 H200-end
• Genbank: NM_003071.3
• Tag: GST
• Species: Human

Sequence of Insert

GTCGACCATGCTGCAGTACAGATGACAACTGAACAGCTTAAAACAGAATT
TGACAAATTGTTTGAAGATTTAAAAGAAGATGATAAAACCCATGAAATGG
AACCAGCTGAGGCTATTGAAACACCACTGCTTCCACATCAAAAACAAGCT
CTAGCTTGGATGGTGTCACGGGAAAATAGCAAAGAACTTCCACCATTCTG
GGAACAGCGAAATGACTTATACTATAACACAATAACAAATTTTTCTGAAA
AGGACCGACCAGAAAATGTCCATGGAGGAATTTTAGCTGATGATATGGGT
TTGGGTAAAACTCTTACAGCCATTGCAGTAATCCTTACCAACTTCCATGA
TGGCAGACCTCTTCCTATTGAAAGAGTTAAAAAGAATCTACTGAAGAAGG
AATATAATGTTAACGATGACTCTATGAAACTTGGAGGAAACAATACCAGT
GAAAAGGCAGATGGACTAAGCAAAGACGCATCTAGATGTAGTGAACAACC
CAGTATTTCAGATATCAAGGAGAAGAGTAAGTTTCGCATGTCAGAATTGT
CTAGCTCCCGCCCCAAAAGAAGAAAAACTGCTGTCCAGTACATAGAAAGC
AGTGATTCAGAGGAAATTGAAACAAGTGAATTGCCGCAGAAAATGAAAGG
CAAACTGAAAAATGTACAGTCTGAAACTAAAGGCAGGGCGAAAGGATCTT
CTAAGGTTATAGAAGATGTGGCATTTGCATGTGCATTAACTTCATCTGTT
CCTACAACAAAAAAGAAAATGTTGAAAAAGGGAGCTTGTGCAGTGGAGGG
GTCAAAGAAAACTGATGTTGAGGAGAGACCAAGAACAACACTGATCATCT
GTCCGCTTTCTGTGTTAAGCAACTGGATTGACCAGTTTGGACAACATATA
AAATCAGATGTACACTTGAATTTTTATGTTTATTATGGTCCTGATCGTAT
TAGAGAACCGGCCTTACTTTCAAAACAGGATATTGTTTTGACTACGTATA
ATATTTTAACTCATGACTATGGAACTAAAGGAGATAGTCCATTACATAGC
ATAAGGTGGCTAAGAGTGATCCTGGATGAAGGACATGCCATACGAAATCC
AAATGCTCAGCAGACAAAAGCTGTACTTGACTTAGAATCAGAAAGAAGAT
GGGTTTTGACAGGTACTCCAATCCAGAATTCTTTAAAGGACTTGTGGTCT
CTTCTTTCCTTTTTAAAACTTAAACCATTTATTGATAGAGAATGGTGGCA
TAGAACAATACAGCGTCCTGTCACAATGGGAGATGAAGGAGGACTTAGGC
GTTTACAGTCCCTAATTAAAAATATTACACTTAGAAGAACAAAGACAAGC
AAAATTAAAGGAAAACCTGTTTTGGAGTTACCAGAACGTAAAGTATTTAT
TCAGCACATTACACTTTCAGATGAAGAGAGAAAGATTTATCAGTCTGTGA
AAAATGAAGGCAGAGCCACTATTGGAAGGTATTTTAATGAAGGGACTGTC
CTGGCACATTATGCAGATGTCCTGGGTCTTTTGCTTAGACTGCGGCAAAT
TTGTTGCCATACTTACCTTCTTACAAATGCAGTGTCTTCCAATGGCCCCT
CAGGAAATGATACACCTGAAGAACTGAGAAAGAAGTTAATAAGGAAGATG
AAGTTAATTCTGAGCTCAGGTTCAGATGAGGAATGTGCAATTTGCCTGGA
TTCTTTAACAGTTCCTGTGATAACACATTGTGCACATGTATTTTGTAAAC
CCTGTATTTGCCAAGTCATTCAGAATGAGCAGCCACATGCTAAATGCCCT
TTATGCAGAAATGATATACATGAAGATAATTTATTAGAATGTCCTCCAGA
AGAATTAGCACGTGACAGTGAGAAAAAGTCTGATATGGAATGGACATCCA
GTTCAAAGATTAATGCGCTAATGCACGCATTGACTGACTTAAGAAAGAAG
AATCCCAACATAAAAAGTTTGGTTGTTTCTCAGTTTACAACATTCCTGTC
TTTAATAGAAATACCACTTAAAGCCTCTGGATTTGTGTTTACTCGTTTGG
ATGGTTCCATGGCCCAAAAGAAAAGAGTTGAATCAATTCAGTGTTTTCAA
AACACTGAAGCAGGATCTCCAACTATAATGCTTCTGTCCTTAAAAGCAGG
TGGAGTTGGTTTGAATCTGTCTGCAGCTTCTCGAGTGTTTTTAATGGATC
CAGCCTGGAATCCTGCTGCTGAAGATCAGTGCTTTGACAGATGCCATAGA
CTTGGTCAGAAGCAAGAAGTTATCATCACAAAATTCATTGTAAAGGACTC
TGTTGAAGAAAATATGCTGAAAATACAAAACAAAAAGAGAGAACTTGCAG
CAGGAGCCTTTGGAACTAAAAAACCAAATGCTGACGAAATGAAACAAGCC
AAAATTAATGAAATCAGAACATTAATTGACTTATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDHAAVQMTTEQLKT
EFDKLFEDLKEDDKTHEMEPAEAIETPLLPHQKQALAWMVSRENSKELPP
FWEQRNDLYYNTITNFSEKDRPENVHGGILADDMGLGKTLTAIAVILTNF
HDGRPLPIERVKKNLLKKEYNVNDDSMKLGGNNTSEKADGLSKDASRCSE
QPSISDIKEKSKFRMSELSSSRPKRRKTAVQYIESSDSEEIETSELPQKM
KGKLKNVQSETKGRAKGSSKVIEDVAFACALTSSVPTTKKKMLKKGACAV
EGSKKTDVEERPRTTLIICPLSVLSNWIDQFGQHIKSDVHLNFYVYYGPD
RIREPALLSKQDIVLTTYNILTHDYGTKGDSPLHSIRWLRVILDEGHAIR
NPNAQQTKAVLDLESERRWVLTGTPIQNSLKDLWSLLSFLKLKPFIDREW
WHRTIQRPVTMGDEGGLRRLQSLIKNITLRRTKTSKIKGKPVLELPERKV
FIQHITLSDEERKIYQSVKNEGRATIGRYFNEGTVLAHYADVLGLLLRLR
QICCHTYLLTNAVSSNGPSGNDTPEELRKKLIRKMKLILSSGSDEECAIC
LDSLTVPVITHCAHVFCKPCICQVIQNEQPHAKCPLCRNDIHEDNLLECP
PEELARDSEKKSDMEWTSSSKINALMHALTDLRKKNPNIKSLVVSQFTTF
LSLIEIPLKASGFVFTRLDGSMAQKKRVESIQCFQNTEAGSPTIMLLSLK
AGGVGLNLSAASRVFLMDPAWNPAAEDQCFDRCHRLGQKQEVIITKFIVK
DSVEENMLKIQNKKRELAAGAFGTKKPNADEMKQAKINEIRTLIDL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11472
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; Sequence differences compared with NM_003071.3 CDS: ; g840a (silent); g909a (silent); delta agc nt1284-1286 (delta residue A429) *; ; Estimated MW of expressed protein : 118977 Daltons; ; ; *derives from EST. also deleted in some published seqs.; Grow at or below 30ºC
• Low Temp: Yes