cDNA Clone ESC4 (yeast)

• Synonyms: RTTFL
• Plasmid: pRS415 Pro-15HA-ESC4
• Parent Plasmid: pRS415
• Genbank: NP_012024
• Tag: HA
• Species: Yeast

Sequence of Insert

GGATCCTCTCCAATAAGATATCCCAATAAGCTATTGAGAATTTCAAGCGC
GTGAAATTCAAATTTATCATCAGAGAACGATGTGCGGGAACTCCAGGGCT
TTTCTGTATTTTCTCATAGACATTATTTATCAGTAATTGCAGCTGTGTAA
CAATAAAATTGACGCCACTTTCTTCGTCTTGTAGCTCCTCGTTAAGCTGC
GAAATTAACCTGCTCGTATTCACAGTAACACACTTAACCACAGAATGTTC
TTCGACATTAGAAATCTCTTGTATTTTTTGTACATCCCAAAAGAATTCAG
ACATTTCTATTGAACCATTCCAGAAATGTTGTTCCAGAAGTTTATTCTCT
TGAACCTTTACACTATTTCGTATTTTCTCGGTTTTAGCCGCCCACTTTTC
ACGCGTTAACAGGGTAAGCTATATCAGATGTGACTTTTCTTTTTCACTCA
TCTAATGTTGTTTTTCGCGTCACATTTTTTGCCAATAAATAAATACATCA
CAATGTTCTATATTGTATATTCAATGGAAGTCATTAGGTTATTGGGCAAG
ATTTCAAGGAGGTAGCATACTGGTAAATATACGATCCATGGGCTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTACGCTGCTCAGTGCGGCCGCATCTTTTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTACGCTGCTATGGGCTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTACGCTGCTATGGGCTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTACGCTGCTATGGGCTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTACGCTGCCATGGGCACATCACTTCTGTTTGAGCAGCTAAATTTTTTAATTTTAGTGGCAGCAGAGGCTGAGCTTCCCATCGCTCACTCTACTAGAAAGCTTTTGATGGATAATTCATGCAATAACTGTCAAATATACGAGCTTTATAACGAAAATTTGAAAGATGTCAAGACGGATAAAGATTGGTTCATGAACAAATTCGGCCCGCAAACAGTACATTTTGTCATTAGTAATACAATCAACTTTCCTTTTTATAAGATAGTTTATTTCGATCTTTTGATCCCGGTGGTATCACACACTTGGGTCCAGGATTCGGTCAAGACGAAGAGACATTTGAGGACAAATATGTATTCACCTAACCCATTTCATTTGCTCCGAGACTGTCAAGTATATATTTCCAAAAGCTCGTTTAATAAGTGTGAATACATATTATACTCTGATTTGCTTCATTTATTAGGAGGCACTTTAGTCAATTACATCTCAAATAGAACGACGCATGTGATTGTTCAAAGTCCCCAGGACCCGATCATAGCAACGGTCAGTAAATTGACATTTGGCTCTTTTTCCTCTTCATCGACCAACAAGCACACCGAAAAGCCTTTGAGAGAGTGGAAATTTGTCTATCCAATATGGATATTATATCATTTCAAAATGGCGAAGCCCTTGAAGGGCGAGCTTGCTACTTTGTGTGAGCTTGATATGCAGGACACCAGTGAGGAACAGTTATTCGCAAAATGGGAAGAGGTTATTGGCGATAAGCAGACATCATCATCGCAGCTTACTCTTCATCCAAACAAAACTTTATTCAAAAATCACCACTTTGCTATCTCACCAGATTTAAACTTTTTCACACCTTTGTATTGGTTTTTGAAAGGATTTATCGAGGATTTGGACGGCAAAGTAACTCCGTTGAGTTTTTCTGACGATCTGAAGTCTGTTTACCAAGCTTTTCCTGATATTGACTGTTATATCGGCCACTCTGCTAACTCACCGATACTAGAAAAAACCAAAAGCATTAAACCGGAAATTCATGTTGGGAACGTAAGTTGGTTGTTTTATATGTTTGCGCTGCAAAAATTTACACCTGTATCGCAATGTAAACTTATTCACCAACCATTTCATGCGAAATTATTTACTTCTAAGGAATTAACAGTCGCGTACACTAATTATTTCGGCTCCCAAAGGTTTTATATTCAGAGGTTAGTAGAAATATTAGGAGGACTTAGCACACCTGAACTAACAAGAAAAAACACACACTTGATCACGAAGAGTACAATTGGCAAAAAATTCAAAGTCGCAAAAAAATGGTCACTGGACCCGCAAAATGCCATTATTGTGACAAATCATATGTGGTTAGAGCAATGTTATATGAATAATTCCAAGTTAAACCCCAAAGACAGTAGATTTCAAAATTTTAAATTGGATGATAACATGGGATGGAATATCGGCCAAATAGGAATGGATCATTCATCTCTCCCAACTCCAAAAAACCTATCGATGGTGACTTACGACACGCAATCCATATCAGAGAAGCCGCCTCCCACAAATGATCAAATGGATCAAATTAACGATAACACCAATGTATTATCAAAGAAAGATGGTACGCCCATTAGTAGTTTTGAGAACTCAATTGATGAAAAGATTGACAAATTACAAAAAATATCAGGAGAGGTGGCTGTAACCCATTCTGGAGATTTAGAACGGAGTTTTGTTTCCAGACCTTCAAGGGCAAGTTTTCCAGTAGTTGACAGTAAAAAATCTAATTTACAAAAGAAAGACTCAAACTCAGATATTAGTATGGAAACAGAGGTTTTCTGTGAAGGACATGAAAAAAGAGAAGAAAAGGAGTTTACGAAACCTATTACGGAGTATGATGCACCTAAGAAACAAGAAATAAGGGAACAGTCACGCAAGAAGAATGACATAGATTATAAAAAAGAAGAGGAAGAAACGGAGCTGCAAGTTCAGCTAGGACAACGAACTAAACGAGAGATCAAAACTTCGAAGAAAAATGAAAAGGAGAAAGAAACCAATGAATGTCACATTGAAGTAGATCAAATGACGAATGAAAAGCAAGGGGAGGAAAGTACAGGAAAACTGATTTCTACGGAAGACGTCACTTCTAAAAAAGATACAGATAAATTCTCGCATTTGTTTGAAGGATTATCTGATAATGATGATCACATTAATGACGAAAAACCGGCAGTTAATTCCAAATATACCACACCCAAGACGTCTCAAAATATTACGAGTGGCGTTGATACTCCAACCACCGCGCAAACGCAAGTTTTTATGCCATCAAGTGGCAATTCAAGGTTAGCAAAGACACAAGCTGCGAAGAGATTGCATACAGATATTGAATCATTAAATGA
ATTTCAGAAAAACTTTAAAAGGAAGAGAATTGATAGCGAGGAGATTTCAT
TGTCCCAAGATGTTGAAAGGTCAAATAATAACAAAGAACTGGCTACTAAA
GCGGAAAAGATATTAGCTAGATTTAATGAACTACCAAATTATGACCTAAA
AGCGGTTTGCACCGGTTGTTTCCATGATGGATTTAATGAGGTTGACATCG
AAATATTGAACCAACTAGGTATAAAAATTTTTGACAATATTAAAGAAACG
GATAAATTGAATTGTATTTTTGCTCCAAAGATTTTGAGAACCGAGAAATT
TTTAAAAAGCTTAAGTTTTGAACCGTTAAAATTCGCATTGAAACCTGAAT
TTATTATTGATTTACTGAAACAAATTCATTCTAAAAAAGATAAACTTTCT
CAAATAAATATAAACTTGTTTGATTATGAGATAAATGGAATTAATGAGAG
TATAATATCAAAAACCAAACTACCTACTAAGGTGTTCGAAAGAGCGAATA
TTAGATGCATTAACTTAGTCAATGATATACCGGGTGGTGTAGATACAATA
GGCTCTGTTCTAAAGGCACATGGTATCGAAAAAATCAACGTTTTACGTAG
TAAGAAATGCACATTTGAAGACATAATCCCAAATGACGTTTCTAAGCAGG
AAAACGGAGGCATTTTTAAATACGTACTTATCGTCACCAAAGCTTCACAG
GTTAAAAAATTTACCAAACTGATTAATGATCGTGATAAAAACGAAACAAT
TTTGATTGTTGAATGGAATTGGTGTGTTGAATCAATTTTTCATCTCAATG
TTGATTTTACATCAAAGAAAAATGTTTTGTATCAAAAGAAAAATAACTAA
GTCGAC

Amino Acid Sequence

MGYPYDVPDYAGYPYDVPDYAGSYPYDVPDYAAQCGRIFYPYDVPDYAGY
PYDVPDYAGSYPYDVPDYAAMGYPYDVPDYAGYPYDVPDYAGSYPYDVPD
YAAMGYPYDVPDYAGYPYDVPDYAGSYPYDVPDYAAMGYPYDVPDYAGYP
YDVPDYAGSYPYDVPDYAAMGTSLLFEQLNFLILVAAEAELPIAHSTRKL
LMDNSCNNCQIYELYNENLKDVKTDKDWFMNKFGPQTVHFVISNTINFPF
YKIVYFDLLIPVVSHTWVQDSVKTKRHLRTNMYSPNPFHLLRDCQVYISK
SSFNKCEYILYSDLLHLLGGTLVNYISNRTTHVIVQSPQDPIIATVSKLT
FGSFSSSSTNKHTEKPLREWKFVYPIWILYHFKMAKPLKGELATLCELDM
QDTSEEQLFAKWEEVIGDKQTSSSQLTLHPNKTLFKNHHFAISPDLNFFT
PLYWFLKGFIEDLDGKVTPLSFSDDLKSVYQAFPDIDCYIGHSANSPILE
KTKSIKPEIHVGNVSWLFYMFALQKFTPVSQCKLIHQPFHAKLFTSKELT
VAYTNYFGSQRFYIQRLVEILGGLSTPELTRKNTHLITKSTIGKKFKVAK
KWSLDPQNAIIVTNHMWLEQCYMNNSKLNPKDSRFQNFKLDDNMGWNIGQ
IGMDHSSLPTPKNLSMVTYDTQSISEKPPPTNDQMDQINDNTNVLSKKDG
TPISSFENSIDEKIDKLQKISGEVAVTHSGDLERSFVSRPSRASFPVVDS
KKSNLQKKDSNSDISMETEVFCEGHEKREEKEFTKPITEYDAPKKQEIRE
QSRKKNDIDYKKEEEETELQVQLGQRTKREIKTSKKNEKEKETNECHIEV
DQMTNEKQGEESTGKLISTEDVTSKKDTDKFSHLFEGLSDNDDHINDEKP
AVNSKYTTPKTSQNITSGVDTPTTAQTQVFMPSSGNSRLAKTQAAKRLHT
DIESLNEFQKNFKRKRIDSEEISLSQDVERSNNNKELATKAEKILARFNE
LPNYDLKAVCTGCFHDGFNEVDIEILNQLGIKIFDNIKETDKLNCIFAPK
ILRTEKFLKSLSFEPLKFALKPEFIIDLLKQIHSKKDKLSQININLFDYE
INGINESIISKTKLPTKVFERANIRCINLVNDIPGGVDTIGSVLKAHGIE
KINVLRSKKCTFEDIIPNDVSKQENGGIFKYVLIVTKASQVKKFTKLIND
RDKNETILIVEWNWCVESIFHLNVDFTSKKNVLYQKKNN*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11460
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence difference compared with NP_012024 CDS:; S2G due to creation of Nco1 site at ATG.