cDNA Clone SMARCA3

• Plasmid: pCMV5-C-Myc SMARCA3
• Parent Plasmid: pCMV5
• Expressed: cMyc-SMARCA3
• Genbank: NM_003071.3
• Tag: Myc
• Species: Human

Sequence of Insert

ggtaccgccaccATGgaacaaaaacttatttctgaagaagatctgtcctg
gatgttcaagagggatccagtttggaagtacttgcagactgtccagtatg
gagttcatggaaattttccacgcctctcatatccaactttctttccacgt
tttgaattccaagatgttatccctccagatgactttctaactagtgatga
agaagtagattccgttttatttggaagtttgagaggtcatgtggttggac
tacgctattacacgggagtagttaataataatgaaatggttgcattacaa
cgagatcctaataacccttatgataagaatgcaattaaagtaaacaatgt
gaatggaaatcaagttggccatttaaagaaagagcttgcaggtgctttgg
cctatatcatggacaacaaattggcacaaattgaaggggtagttcctttt
ggtgcaaacaatgcttttaccatgcctctgcatatgactttttggggaaa
agaagaaaatagaaaagcggtttcagatcagttgaagaaacatggattta
aattgggtcctgcaccaaaaactttaggattcaatttggaaagtggttgg
ggctctggaagagctggaccaagctatagtatgccagtgcatgctgcagt
acagatgacaactgaacagcttaaaacagaatttgacaaattgtttgaag
atttaaaagaagatgataaaacccatgaaatggaaccagctgaggctatt
gaaacaccactgcttccacatcaaaaacaagctctagcttggatggtgtc
acgggaaaatagcaaagaacttccaccattctgggaacagcgaaatgact
tatactataacacaataacaaatttttctgagaaggaccgaccagaaaat
gtccatggaggaattttagctgatgatatgggtttgggtaaaactcttac
ggccattgcagtaatccttaccaacttccatgatggcagacctcttccta
ttgaaagagttaaaaagaatctactgaagaaggaatataatgttaacgat
gactctatgaaacttggaggaaacaataccagtgaaaaggcagatggact
aagcaaagacgcatctagatgtagtgaacaacccagtatttcagatatca
aggagaagagtaagtttcgcatgtcagaattgtctagctcccgccccaaa
agaagaaaaactgctgtccagtacatagaaagcagtgattcagaggaaat
tgaaacaagtgaattgccgcagaaaatgaaaggcaaactgaaaaatgtac
agtctgaaactaaaggcagggcgaaagcaggatcttctaaggttatagaa
gatgtggcatttgcatgtgcattaacttcatctgttcctacaacaaaaaa
gaaaatgttgaaaaagggagcttgtgcagtggaggggtcaaagaaaactg
atgttgaggagagaccaagaacaacactgatcatctgtccgctttctgtg
ttaagcaactggattgaccagtttggacaacatataaaatcagatgtaca
cttgaatttttatgtttattatggtcctgatcgtattagagaaccggcct
tactttcaaaacaggatattgttttgactacgtataatattttaactcat
gactatggaactaaaggagatagtccattacatagcataaggtggctaag
agtgatcctggatgaaggacatgccatacgaaatccaaatgctcagcaga
caaaagctgtacttgacttagaatcagaaagaagatgggttttgacaggt
actccaatccagaattctttaaaggacttgtggtctcttctttccttttt
aaaacttaaaccatttattgatagagaatggtggcatagaacaatacagc
gtcctgtcacaatgggagatgaaggaggacttaggcgtttacagtcccta
attaaaaatattacacttagaagaacaaagacaagcaaaattaaaggaaa
acctgttttggagttaccagaacgtaaagtatttattcagcacattacac
tttcagatgaagagagaaagatttatcagtctgtgaaaaatgaaggcaga
gccactattggaaggtattttaatgaagggactgtcctggcacattatgc
agatgtcctgggtcttttgcttagactgcggcaaatttgttgccatactt
accttcttacaaatgcagtgtcttccaatggcccctcaggaaatgataca
cctgaagaactgagaaagaagttaataaggaagatgaagttaattctgag
ctcaggttcagatgaggaatgtgcaatttgcctggattctttaacagttc
ctgtgataacacattgtgcacatgtattttgtaaaccctgtatttgccaa
gtcattcagaatgagcagccacatgctaaatgccctttatgcagaaatga
tatacatgaagataatttattagaatgtcctccagaagaattagcacgtg
acagtgagaaaaagtctgatatggaatggacatccagttcaaagattaat
gcgctaatgcacgcattgactgacttaagaaagaagaatcccaacataaa
aagtttggttgtttctcagtttacaacattcctgtctttaatagaaatac
cacttaaagcctctggatttgtgtttactcgtttggatggttccatggcc
caaaagaaaagagttgaatcaattcagtgttttcaaaacactgaagcagg
atctccaactataatgcttctgtccttaaaagcaggtggagttggtttga
atctgtctgcagcttctcgagtgtttttaatggatccagcctggaatcct
gctgctgaagatcagtgctttgacagatgccatagacttggtcagaagca
agaagttatcatcacaaaattcattgtaaaggactctgttgaagaaaata
tgctgaaaatacaaaacaaaaagagagaacttgcagcaggagcctttgga
actaaaaaaccaaatgctgacgaaatgaaacaagccaaaattaatgaaat
cagaacattaattgacttataagtcgac

Amino Acid Sequence

MEQKLISEEDLSWMFKRDPVWKYLQTVQYGVHGNFPRLSYPTFFPRFEFQ
DVIPPDDFLTSDEEVDSVLFGSLRGHVVGLRYYTGVVNNNEMVALQRDPN
NPYDKNAIKVNNVNGNQVGHLKKELAGALAYIMDNKLAQIEGVVPFGANN
AFTMPLHMTFWGKEENRKAVSDQLKKHGFKLGPAPKTLGFNLESGWGSGR
AGPSYSMPVHAAVQMTTEQLKTEFDKLFEDLKEDDKTHEMEPAEAIETPL
LPHQKQALAWMVSRENSKELPPFWEQRNDLYYNTITNFSEKDRPENVHGG
ILADDMGLGKTLTAIAVILTNFHDGRPLPIERVKKNLLKKEYNVNDDSMK
LGGNNTSEKADGLSKDASRCSEQPSISDIKEKSKFRMSELSSSRPKRRKT
AVQYIESSDSEEIETSELPQKMKGKLKNVQSETKGRAKAGSSKVIEDVAF
ACALTSSVPTTKKKMLKKGACAVEGSKKTDVEERPRTTLIICPLSVLSNW
IDQFGQHIKSDVHLNFYVYYGPDRIREPALLSKQDIVLTTYNILTHDYGT
KGDSPLHSIRWLRVILDEGHAIRNPNAQQTKAVLDLESERRWVLTGTPIQ
NSLKDLWSLLSFLKLKPFIDREWWHRTIQRPVTMGDEGGLRRLQSLIKNI
TLRRTKTSKIKGKPVLELPERKVFIQHITLSDEERKIYQSVKNEGRATIG
RYFNEGTVLAHYADVLGLLLRLRQICCHTYLLTNAVSSNGPSGNDTPEEL
RKKLIRKMKLILSSGSDEECAICLDSLTVPVITHCAHVFCKPCICQVIQN
EQPHAKCPLCRNDIHEDNLLECPPEELARDSEKKSDMEWTSSSKINALMH
ALTDLRKKNPNIKSLVVSQFTTFLSLIEIPLKASGFVFTRLDGSMAQKKR
VESIQCFQNTEAGSPTIMLLSLKAGGVGLNLSAASRVFLMDPAWNPAAED
QCFDRCHRLGQKQEVIITKFIVKDSVEENMLKIQNKKRELAAGAFGTKKP
NADEMKQAKINEIRTLIDL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11455
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence difference compared with NM_003071.3 CDS: ; 1. g909a (silent T303); 2. Delta agc nt1284-1286 (delta residue A429*) *derives from EST. Also deleted in some published seqs.; ; Estimated MW of expressed protein : 115110 Daltons; ; ; Grow at or below 30ºC
• Low Temp: Yes