cDNA Clone PCNA (yeast)

• Plasmid: pRS415 Pro-PCNA-6His-3Myc
• Parent Plasmid: pRS415
• Genbank: AY557715.1 GI:45269320
• Tag: 6His
• Species: Yeast

Sequence of Insert

ACTAGTGCAACCAAAGGAACCAAAGAAGAGGACCACCAGGAGAAAGAAGG
ATCCTAACGCCCCTAAGAGGGGCTTGTCAGCTTATATGTTCTTTGCTAAT
GAAAACAGAGACATTGTCCGTTCCGAGAATCCTGACGTAACTTTTGGCCA
AGTAGGCAGAATATTGGGTGAGAGGTGGAAGGCCTTAACTGCTGAAGAAA
AGCAACCCTATGAATCTAAGGCTCAAGCAGACAAGAAGAGATACGAATCT
GAAAAGGAATTGTACAATGCTACACGTGCTTGATCTAACAACTCAATGTT
CTCTACCTCCCATACAACGTACTTTTGAAACTTCAGAAAGAAGTTGAGTA
GGTTGCATTATGTGTTATTATTATTACTATTATCATTATTTTACTTATAT
ATCCTTCTTTTCTTTTATGTATTCTCTTCCTTTTTCGCACAACTTATGCT
GATTCTTCAATATCTAATTATTTAGCATTTTTCTTCTCCATCCGACGCAC
CAAAACGCGAAACGCGTAACTTTTTTTTTTTGGATTTCAACTGATAGTTT
TCGTACTTTGCTTCCTCTGGTACATAAAATTATATATAAGAAACACTTTT
GCTTTAGCCTTCCTTTCTTTCCACTTGCACCTTTCACTTTCGCCGTCCTT
TTTCACTCACAGCAACAAGCAGCAAGCACTAAGTACGCAGTCAAAAGAGA
GAAAAAATGTTAGAAGCAAAATTTGAAGAAGCATCCCTTTTCAAGAGAAT
AATTGATGGTTTCAAAGATTGTGTCCAGTTGGTCAATTTCCAATGTAAAG
AAGATGGTATCATTGCACAAGCTGTCGATGACTCAAGAGTTCTATTGGTC
TCCTTGGAAATAGGTGTCGAAGCCTTCCAAGAATATAGATGTGACCATCC
TGTTACGTTAGGTATGGATCTAACCTCACTAAGTAAAATCCTACGTTGTG
GTAACAACACCGATACATTAACACTAATTGCTGACAACACACCGGATTCC
ATCATCTTATTATTTGAGGATACCAAGAAAGACCGTATAGCCGAATACTC
TCTGAAATTGATGGATATCGATGCTGATTTCTTAAAGATTGAAGAATTAC
AGTACGACTCCACCCTGTCATTGCCATCTTCCGAATTCTCTAAAATTGTT
CGTGACTTGTCCCAATTGAGTGATTCTATTAATATCATGATCACCAAAGA
AACAATAAAGTTTGTAGCTGACGGTGATATCGGATCAGGTTCAGTCATAA
TAAAACCATTCGTGGATATGGAACATCCTGAAACAAGCATCAAACTTGAA
ATGGATCAACCTGTCGACTTGACGTTCGGAGCTAAATATTTATTGGACAT
CATTAAGGGCTCCTCCCTTTCTGATAGAGTTGGTATCAGGCTCTCCAGCG
AAGCTCCTGCTTTATTCCAATTTGATTTGAAGAGTGGGTTCCTACAGTTT
TTCTTGGCTCCTAAATTTAATGACGAAGAACACCATCACCATCACCACGG
TGAACAAAAGCTAATCTCCGAGGAAGACTTGAACGGTGAACAAAAATTAA
TCTCAGAAGAAGACTTGAACGGACTCGACGGTGAACAAAAGTTGATTTCT
GAAGAAGATTTGGGTTAAGCGGCCGC

Amino Acid Sequence

MLEAKFEEASLFKRIIDGFKDCVQLVNFQCKEDGIIAQAVDDSRVLLVSL
EIGVEAFQEYRCDHPVTLGMDLTSLSKILRCGNNTDTLTLIADNTPDSII
LLFEDTKKDRIAEYSLKLMDIDADFLKIEELQYDSTLSLPSSEFSKIVRD
LSQLSDSINIMITKETIKFVADGDIGSGSVIIKPFVDMEHPETSIKLEMD
QPVDLTFGAKYLLDIIKGSSLSDRVGIRLSSEAPALFQFDLKSGFLQFFL
APKFNDEEHHHHHHGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEE
DLG*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11448
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee