cDNA Clone PCNA (yeast)

• Plasmid: pRS415 PCNAE (ATG=Nco1)
• Parent Plasmid: pRS415
• Species: Yeast

Sequence of Insert

ACTAGTGCAACCAAAGGAACCAAAGAAGAGGACCACCAGGAGAAAGAAGGATCCTAACGCCCCTAAGAGGGGCTTGTCAGCTTATATGTTCTTTGCTAATGAAAACAGAGACATTGTCCGTTCCGAGAATCCTGACGTAACTTTTGGCCAAGTAGGCAGAATATTGGGTGAGAGGTGGAAGGCCTTAACTGCTGAAGAAAAGCAACCCTATGAATCTAAGGCTCAAGCAGACAAGAAGAGATACGAATCTGAAAAGGAATTGTACAATGCTACACGTGCTTGATCTAACAACTCAATGTTCTCTAC
CTCCCATACAACGTACTTTTGAAACTTCAGAAAGAAGTTGAGTAGGTTGC
ATTATGTGTTATTATTATTACTATTATCATTATTTTACTTATATATCCTT
CTTTTCTTTTATGTATTCTCTTCCTTTTTCGCACAACTTATGCTGATTCT
TCAATATCTAATTATTTAGCATTTTTCTTCTCCATCCGACGCACCAAAAC
GCGAAACGCGTAACTTTTTTTTTTTGGATTTCAACTGATAGTTTTCGTAC
TTTGCTTCCTCTGGTACATAAAATTATATATAAGAAACACTTTTGCTTTA
GCCTTCCTTTCTTTCCACTTGCACCTTTCACTTTCGCCGTCCTTTTTCAC
TCACAGCAACAAGCAGCAAGCACTAAGTACGCAGTCAAAAGAGAGAAACC
ATGGTAGAAGCAAAATTTGAAGAAGCATCCCTTTTCAAGAGAATAATTGA
TGGTTTCAAAGATTGTGTCCAGTTGGTCAATTTCCAATGTAAAGAAGATG
GTATCATTGCACAAGCTGTCGATGACTCAAGAGTTCTATTGGTCTCCTTG
GAAATAGGTGTCGAAGCCTTCCAAGAATATAGATGTGACCATCCTGTTAC
GTTAGGTATGGATCTAACCTCACTAAGTAAAATCCTACGTTGTGGTAACA
ACACCGATACATTAACACTAATTGCTGACAACACACCGGATTCCATCATC
TTATTATTTGAGGATACCAAGAAAGACCGTATAGCCGAATACTCTCTGAA
ATTGATGGATATCGATGCTGATTTCTTAAAGATTGAAGAATTACAGTACG
ACTCCACCCTGTCATTGCCATCTTCCGAATTCTCTAAAATTGTTCGTGAC
TTGTCCCAATTGAGTGATTCTATTAATATCATGATCACCAAAGAAACAAT
AAAGTTTGTAGCTGACGGTGATATCGGATCAGGTTCAGTCATAATAAAAC
CATTCGTGGATATGGAACATCCTGAAACAAGCATCAAACTTGAAATGGAT
CAACCTGTCGACTTGACGTTCGGAGCTAAATATTTATTGGACATCATTAA
GGGCTCCTCCCTTTCTGATAGAGTTGGTATCAGGCTCTCCAGCGAAGCTC
CTGCTTTATTCCAATTTGATTTGAAGAGTGGGTTCCTACAGTTTTTCTTG
GCTCCTAAATTTAATGACGAAGAATAAATGTAAATTATCTATATAGTTGT
ATACTAAAAATAATAAACAAAAAAAAAACAGTAAAGTTTGTTTTAAATGA
AAATAAATAACAAAGAAAATAAAGACTGCGGCCGC

Amino Acid Sequence

MLEAKFEEASLFKRIIDGFKDCVQLVNFQCKEDGIIAQAVDDSRVLLVSL
EIGVEAFQEYRCDHPVTLGMDLTSLSKILRCGNNTDTLTLIADNTPDSII
LLFEDTKKDRIAEYSLKLMDIDADFLKIEELQYDSTLSLPSSEFSKIVRD
LSQLSDSINIMITKETIKFVADGDIGSGSVIIKPFVDMEHPETSIKLEMD
QPVDLTFGAKYLLDIIKGSSLSDRVGIRLSSEAPALFQFDLKSGFLQFFL
APKFNDEE*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11427
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast expression plasmid for untagged PCNA.; Differences from ref sequence:; c65g, g67c in promoter seq.; L2V due to Nco1 site at ATG.