cDNA Clone PAP2

• Plasmid: pGEX6P-3-PAP2
• Parent Plasmid: pGEX6P3
• Expressed: GST-PAP2
• Genbank: NM_032632
• Tag: GST
• Species: Human

Sequence of Insert

ggatccatgccgtttccagttacaacgcagggatcacaacaaacacaaccgccacagaagcactatggcattacttctcctatcagcttagcagcccccaaggagactgactgcgtacttacacagaaactaattgagacattgaaaccctttggggtttttgaagaggaagaggaactgcagcgcaggattttaattttgggaaaactaaataacctggtaaaagagtggatacgagaaatcagtgaaagcaagaatcttccacaatctgtaattgaaaatgttggaggaaaaatttttacattt
ggatcttacagattaggagtgcatacaaaaggtgctgatattgatgcgtt
gtgtgttgcaccaagacatgttgatcgaagtgactttttcacctcattct
atgataagttgaaattacaggaagaagtaaaagatttaagagctgttgaa
gaggcattcgtaccagttattaaactctgttttgatgggatagagattga
tattttgtttgcaagattagcactgcagacaattcctgaagatttggatc
tacgagatgacagtctgctaaaaaatttagatataagatgtataagaagt
cttaacggttgcagggtaaccgatgaaattttacatctagtaccaaacat
tgacaacttcaggttaactctgagagctatcaaactatgggccaaacgcc
acaacatctattccaatatattaggtttcctcggtggtgtttcctgggct
atgctagtagcaagaacttgccagctttatccaaatgcaatagcatcaac
tcttgtacataaatttttcttggtattttctaaatgggaatggccaaatc
cagtgctattgaaacagcctgaagaatgcaatcttaatttgcctgtatgg
gacccaagggtaaaccccagtgataggtaccatcttatgcctataattac
accagcatacccacaacagaactccacgtacaatgtgtccgtttcaacac
ggatggtcatggttgaggagtttaaacaaggtcttgctatcacagatgaa
attttgctgagtaaggcagagtggtccaaactttttgaagctccaaactt
ctttcaaaagtacaagcattatattgtacttctagcaagtgcaccaacag
aaaaacaacgcctggaatgggtgggcttggtggaatcaaaaatccgaatc
ctggttggaagcttggagaagaatgaatttattacactggctcatgtgaa
tccccagtcatttccagcacccaaagaaaatcccgacaaggaagaatttc
gcacgatgtgggtgattgggttagtgtttaaaaaaacagaaaactctgaa
aacctcagtgttgatctcacctatgatattcagtctttcacagatacagt
ttataggcaagcaataaacagcaagatgtttgaggtggatatgaaaattg
ctgcaatgcatgtaaaaagaaagcaactccatcaactactacctaatcat
gtgcttcagaaaaagaaaaagcattcaacagaaggtgtcaaattgacagc
tctcaatgacagcagcctcgacttgtctatggacagtgataacagcatgt
ctgtgccttcacctactagtgctacgaagaccagtccattgaacagttct
ggcagctctcagggcagaaacagtcctgctccagctgtaacagcagcatc
tgtgaccaacatacaggctactgaagtttctgtgccacaagtaaattcca
gtgaaagctcagggggtacatcgagtgaaagcattcctcaaactgccaca
caaccagccatttctccaccaccaaagcctacggtctccagagttgtttc
ttcaacacgtctggtaaacccaccacctagatcttcaggaaatgcagcaa
cttcaggaaatgcagcaacaaaaatacctactcctatagtaggagtcaag
aggacatcctcacctcataaagaagagagtcccaagaaaaccaaaacaga
agaggatgaaacaagtgaagatgctaactgtcttgctttgagtggacatg
ataaaacagaagcaaaggaacaacttgatacagagacaagtacaactcaa
tcagaaactattcagacagcggcttctctgttggcctctcagaaaacatc
cagtacagacctttctgatatccctgctctccctgcaaatcctattcctg
ttatcaagaattcaataaaactgagactgaatcggtaagcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMPFPVTTQGSQQTQPPQKH
YGITSPISLAAPKETDCVLTQKLIETLKPFGVFEEEEELQRRILILGKLN
NLVKEWIREISESKNLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCV
APRHVDRSDFFTSFYDKLKLQEEVKDLRAVEEAFVPVIKLCFDGIEIDIL
FARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLVPNIDN
FRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLV
HKFFLVFSKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPA
YPQQNSTYNVSVSTRMVMVEEFKQGLAITDEILLSKAEWSKLFEAPNFFQ
KYKHYIVLLASAPTEKQRLEWVGLVESKIRILVGSLEKNEFITLAHVNPQ
SFPAPKENPDKEEFRTMWVIGLVFKKTENSENLSVDLTYDIQSFTDTVYR
QAINSKMFEVDMKIAAMHVKRKQLHQLLPNHVLQKKKKHSTEGVKLTALN
DSSLDLSMDSDNSMSVPSPTSATKTSPLNSSGSSQGRNSPAPAVTAASVT
NIQATEVSVPQVNSSESSGGTSSESIPQTATQPAISPPPKPTVSRVVSST
RLVNPPPRSSGNAATSGNAATKIPTPIVGVKRTSSPHKEESPKKTKTEED
ETSEDANCLALSGHDKTEAKEQLDTETSTTQSETIQTAASLLASQKTSST
DLSDIPALPANPIPVIKNSIKLRLNR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11267
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; Sequence differences compared with NM_032632 CDS: a21g, t2227c (both silent).; ; Estimated MW of expressed protein: 109663 Daltons