cDNA Clone TBC1D1

• Plasmid: pCTAP TBC1D1
• Parent Plasmid: pCTAP-A
• Expressed: TBC1D1-TAP
• Genbank: NM_015173
• Tag: TAP
• Species: Human

Sequence of Insert

CCGCGGGCCACCATGGAACCAATAACATTCACAGCAAGGAAACATCTGCTTTCTAACGAGGTCTCGGTGGATTTTGGCCTGCAGCTGGTGGGCTCCCTGCCTGTGCATTCCCTGACCACCATGCCCATGCTGCCCTGGGTTGTGGCTGAGGTGCGAAGACTCAGCAGGCAGTCCACCAGAAAGGAACCTGTAACCAAGCAAGTCCGGCTTTGCGTTTCACCCTCTGGACTGAGATGTGAACCTGAGCCAGGGAGAAGTCAACAGTGGGATCCCCTGATCTATTCCAGCATCTTTGAGTGCAAGCCTCAGCGTGTTCACAAACTGATTCACAACAGTCATGACCCAAGTTACTTTGCTTGTCTGATTAAGGAAGACGCTGTCCACCGGCAGAGTATCTGCTATGTGTTCAAAGCCGATGATCAAACAAAAGTGCCTGAGATCATCAGCTCCATCCGTCAGGCGGGGAAGATCGCCCGGCAGGAGGAGCTGCACTGCCCGTCCGAGTTCGACGACACGTTTTCCAAGAAGTTCGAGGTGCTCTTCTGCGGCCGCGTGACGGTGGCGCACAAGAAGGCTCCGCCGGCCCTGATCGACGAGTGCATCGAGAAGTTC
AATCACGTCAGCGGCAGCCGGGGGTCCGAGAGCCCCCGCCCCAACCCGCC
CCATGCCGCGCCCACAGGGAGCCAGGAGCCTGTGCGCAGGCCCATGCGCA
AGTCCTTCTCCCAGCCCGGCCTGCGCTCGCTGGCCTTTAGGAAGGAGCTG
CAGGATGGGGGCCTCCGAAGCAGCGGCTTCTTCAGCTCCTTCGAGGAGAG
CGACATTGAGAACCACCTCATTAGCGGACACAATATTGTGCAGCCCACAG
ATATCGAGGAAAATCGAACTATGCTCTTCACGATTGGCCAGTCTGAAGTT
TACCTCATCAGTCCTGACACCAAAAAAATAGCATTGGAGAAAAATTTTAA
GGAGATATCCTTTTGCTCTCAGGGCATCAGACACGTGGACCACTTTGGGT
TTATCTGTCGGGAGTCTTCCGGAGGTGGCGGCTTTCATTTTGTCTGTTAC
GTGTTTCAGTGCACAAATGAGGCTCTGGTTGATGAAATTATGATGACCCT
GAAACAGGCCTTCACGGTGGCCGCAGTGCAGCAGACAGCTAAGGCGCCAG
CCCAGCTGTGTGAGGGCTGCCCCCTGCAAAGCCTGCACAAGCTCTGTGAG
AGGATAGAGGGAATGAATTCTTCCAAAACAAAACTAGAACTGCAAAAGCA
CCTGACGACATTAACAAATCAGGAGCAGGCGACTATTTTTGAAGAGGTTC
AGAAATTGAGACCGAGAAATGAGCAGCGAGAGAATGAATTGATTATTTCT
TTTCTGAGATGTTTATATGAAGAGAAACAGAAAGAACACATCCATATTGG
GGAGATGAAGCAGACATCGCAGATGGCAGCAGAGAATATTGGAAGTGAAT
TACCACCCAGTGCCACTCGATTTAGGCTAGATATGCTGAAAAACAAAGCA
AAGAGATCTTTAACAGAGTCTTTAGAAAGTATTTTGTCCCGGGGTAATAA
AGCCAGAGGCCTGCAGGAACACTCCATCAGTGTGGATCTGGATAGCTCCC
TGTCTAGTACATTAAGTAACACCAGCAAAGAGCCATCTGTGTGTGAAAAG
GAGGCCTTGCCCATCTCTGAGAGCTCCTTTAAGCTCCTCGGCTCCTCGGA
GGACCTGTCCAGTGACTCGGAGAGTCATCTCCCAGAAGAGCCAGCTCCGC
TGTCGCCCCAGCAGGCCTTCAGGAGGCGAGCAAACACCCTGAGTCACTTC
CCCATCGAATGCCAGGAACCTCCACAACCTGCCCGGGGGTCCCCGGGGGT
TTCGCAAAGGAAACTTATGAGGTATCACTCAGTGAGCACAGAGACGCCTC
ATGAACGAAAGGACTTTGAATCCAAAGCAAACCATCTTGGTGATTCTGGT
GGGACTCCTGTGAAGACCCGGAGGCATTCCTGGAGGCAGCAGATATTCCT
CCGAGTAGCCACCCCGCAGAAGGCGTGCGATTCTTCCAGCAGATATGAAG
ATTATTCAGAGCTGGGAGAGCTTCCCCCACGATCTCCTTTAGAACCAGTT
TGTGAAGATGGGCCCTTTGGCCCCCCACCAGAGGAAAAGAAAAGGACATC
TCGTGAGCTCCGAGAGCTGTGGCAAAAGGCTATTCTTCAACAGATACTGC
TGCTTAGAATGGAGAAGGAAAATCAGAAGCTCCAAGCCTCTGAAAATGAT
TTGCTGAACAAGCGCCTGAAGCTCGATTATGAAGAAATTACTCCCTGTCT
TAAAGAAGTAACTACAGTGTGGGAAAAGATGCTTAGCACTCCAGGAAGAT
CAAAAATTAAGTTTGACATGGAAAAAATGCACTCGGCTGTTGGGCAAGGT
GTGCCACGTCATCACCGAGGTGAAATCTGGAAATTTCTAGCTGAGCAATT
CCACCTTAAACACCAGTTTCCCAGCAAACAGCAGCCAAAGGATGTGCCAT
ACAAAGAACTCTTAAAGCAGCTGACTTCCCAGCAGCATGCGATTCTTATT
GACCTTGGGCGAACCTTTCCTACACACCCATACTTCTCTGCCCAGCTTGG
AGCAGGACAGCTATCGCTTTACAACATTTTGAAGGCCTACTCACTTCTAG
ACCAGGAAGTGGGATATTGCCAAGGTCTCAGCTTTGTAGCAGGCATTTTG
CTTCTTCATATGAGTGAGGAAGAGGCGTTTAAAATGCTCAAGTTTCTGAT
GTTTGACATGGGGCTGCGGAAACAGTATCGGCCAGACATGATTATTTTAC
AGATCCAGATGTACCAGCTCTCGAGGTTGCTTCATGATTACCACAGAGAC
CTCTACAATCACCTGGAGGAGCACGAGATCGGCCCCAGCCTCTACGCTGC
CCCCTGGTTCCTCACCATGTTTGCCTCACAGTTCCCGCTGGGATTCGTAG
CCAGAGTCTTTGATATGATTTTTCTTCAGGGAACAGAGGTCATATTTAAA
GTGGCTTTAAGTCTGTTGGGAAGCCATAAGCCCTTGATTCTGCAGCATGA
AAACCTAGAAACCATAGTTGACTTTATAAAAAGCACGCTACCCAACCTTG
GCTTGGTACAGATGGAAAAGACCATCAATCAGGTATTTGAAATGGACATC
GCTAAACAGTTACAAGCTTATGAAGTTGAGTACCACGTCCTTCAAGAAGA
ACTTATCGATTCCTCTCCTCTCAGTGACAACCAAAGAATGGATAAATTAG
AGAAAACCAACAGCAGCTTACGCAAACAGAACCTTGACCTCCTTGAACAG
TTGCAGGTGGCAAATGGTAGGATCCAAAGCCTTGAGGCCACCATTGAGAA
GCTCCTGAGCAGTGAGAGCAAGCTGAAGCAGGCCATGCTTACCTTAGAAC
TGGAGCGGTCGGCCCTGCTGCAGACGGTGGAGGAGCTGCGGCGGCGGAGC
GCAGAGCCCAGCGACCGGGAGCCTGAGTGCACGCAGCCCGAGCCCACGGG
CGACGTCGAC

Amino Acid Sequence

MEPITFTARKHLLSNEVSVDFGLQLVGSLPVHSLTTMPMLPWVVAEVRRL
SRQSTRKEPVTKQVRLCVSPSGLRCEPEPGRSQQWDPLIYSSIFECKPQR
VHKLIHNSHDPSYFACLIKEDAVHRQSICYVFKADDQTKVPEIISSIRQA
GKIARQEELHCPSEFDDTFSKKFEVLFCGRVTVAHKKAPPALIDECIEKF
NHVSGSRGSESPRPNPPHAAPTGSQEPVRRPMRKSFSQPGLRSLAFRKEL
QDGGLRSSGFFSSFEESDIENHLISGHNIVQPTDIEENRTMLFTIGQSEV
YLISPDTKKIALEKNFKEISFCSQGIRHVDHFGFICRESSGGGGFHFVCY
VFQCTNEALVDEIMMTLKQAFTVAAVQQTAKAPAQLCEGCPLQSLHKLCE
RIEGMNSSKTKLELQKHLTTLTNQEQATIFEEVQKLRPRNEQRENELIIS
FLRCLYEEKQKEHIHIGEMKQTSQMAAENIGSELPPSATRFRLDMLKNKA
KRSLTESLESILSRGNKARGLQEHSISVDLDSSLSSTLSNTSKEPSVCEK
EALPISESSFKLLGSSEDLSSDSESHLPEEPAPLSPQQAFRRRANTLSHF
PIECQEPPQPARGSPGVSQRKLMRYHSVSTETPHERKDFESKANHLGDSG
GTPVKTRRHSWRQQIFLRVATPQKACDSSSRYEDYSELGELPPRSPLEPV
CEDGPFGPPPEEKKRTSRELRELWQKAILQQILLLRMEKENQKLQASEND
LLNKRLKLDYEEITPCLKEVTTVWEKMLSTPGRSKIKFDMEKMHSAVGQG
VPRHHRGEIWKFLAEQFHLKHQFPSKQQPKDVPYKELLKQLTSQQHAILI
DLGRTFPTHPYFSAQLGAGQLSLYNILKAYSLLDQEVGYCQGLSFVAGIL
LLHMSEEEAFKMLKFLMFDMGLRKQYRPDMIILQIQMYQLSRLLHDYHRD
LYNHLEEHEIGPSLYAAPWFLTMFASQFPLGFVARVFDMIFLQGTEVIFK
VALSLLGSHKPLILQHENLETIVDFIKSTLPNLGLVQMEKTINQVFEMDI
AKQLQAYEVEYHVLQEELIDSSPLSDNQRMDKLEKTNSSLRKQNLDLLEQ
LQVANGRIQSLEATIEKLLSSESKLKQAMLTLELERSALLQTVEELRRRS
AEPSDREPECTQPEPTGDVDLEGSGSGTMDEKTTGWRGGHVVEGLAGELE
QLRARLEHHPQGQREPSGGCKLGSGKRRWKKNFIAVSAANRFKKISSSGA
L*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU11258
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression vector for expression of TBC1D1 with C-terminal TAP tag.; Sequence difference compared with NM_015173 CDS:; C1266a (silent in T422)