cDNA Clone PI3K beta

• Plasmid: pFastBacDual 6His.PI3K beta/p85
• Parent Plasmid: pFastbacDual
• Expressed: 6His-PI3K beta/p85
• Genbank: Genbank PI3K beta : NM_006219 / Genbank p85 : NM_181523
• Tag: 6His
• Species: Human

Sequence of Insert

DNA sequence of insert 1 :
ctcgagGAAACCATGCATCACCATCACCATCACATGTGCTTCAGTTTCATAATGCCTCCTGCTATGGCAGACATCCTTGACATCTGGGCGGTGGATTCACAGATAGCATCTGATGGCTCCATACCTGTGGATTTCCTTTTGCCCACTGGGATTTATATCCAGTTGGAGGTACCTCGGGAAGCTACCATTTCTTATATTAAGCAGATGTTATGGAAGCAAGTTCACAATTACCCAATGTTCAACCTCCTTATGGATATTGACTCCTATATGTTTGCATGTGTGAATCAGACTGCTGTATATGAGGAGCTTGAAGATGAAACACGAAGACTCTGTGATGTCAGACCTTTTCTTCCAGTTCTCAAATTAGTGACAAGAAGTTGTGACCCAGGGGAAAAATTAGACTCAAAAATTGGAGTCCTTATAGGAAAAGGTCTGCATGAATTTGATTCCTTGAAGGATCCTGAAGTAAATGAATTTCGAAGAAAAATGCGCAAATTCAGCGAGGAAAAAATCCTGTCACTTGTGGGATTGTCTTGGATGGACTGGCTAAAACAAACATATCCACCAGAGCATGAACCATCCATCCCTGAAAACTTAGAAGATAAACTTTATGGGGGAAAGCTCATCGTAGCTGTTCATTTTGAAAACTGCCAGGACGTGTTTAGCTTTCAAGTGTCTCCTAATATGAATCCTATCAAAGTAAATGAATTGGCAATCCAAAAACGTTTGACTATTCATGGGAAGGAAGATGAAGTTAGCCCCTATGATTATGTGTTGCAAGTCAGCGGGAGAGTAGAATATGTTTTTGGTGATCATCCACTAATTCAGTTCCAGTATATCCGGAACTGTGTGATGAACAGAGCCCTGCCCCATTTTATACTTGTGGAATGCTGCAAGATCAAGAAAATGTATGAACAAGAAATGATTGCCATAGAGGCTGCCATAAATCGAAATTCATCTAATCTTCCTCTTCCATTACCACCAAAGAAAACACGAATTATTTCTCATGTTTGGGAAAATAACAACCCTTTCCAAATTGTCTTGGTTAAGGGAAATAAACTTAACACAGAGGAAACTGTAAAAGTTCATGTCAGGGCTGGTCTTTTTCATGGTACTGAGCTCCTGTGTAAAACCATCGTAAGCTCAGAGGTATCAGGGAAAAATGATCATATTTGGAATGAACCACTGGAATTTGATATTAATATTTGTGACTTACCAAGAATGGCTCGATTATGTTTTGCTGTTTATGCAGTTTTGGATAAAGTAAAAACGAAGAAATCAACGAAAACTATTAATCCCTCTAAATATCAGACCATCAGGAAAGCTGGAAAAGTGCATTATCCTGTAGCGTGGGTAAATACGATGGTTTTTGACTTTAAAGGACAATTGAGAACTGGAGACATAATATTACACAGCTGGTCTTCATTTCCTGATGAACTCGAAGAAATGTTGAATCCAATGGGAACTGTTCAAACAAATCCATATACTGAAAATGCAACAGCTTTGCATGTTAAATTTCCAGAGAATAAAAAACAACCTTATTATTACCCTCCCTTCGATAAGATTATTGAAAAGGCAGCTGAGATTGCAAGCAGTGATAGTGCTAATGTGTCAAGTCGAGGTGGAAAAAAGTTTCTTCCTGTATTGAAAGAAATCTTGGACAGGGATCCCTTGTCTCAACTG
TGTGAAAATGAAATGGATCTTATTTGGACTTTGCGACAAGACTGCCGAGA
GATTTTCCCACAATCACTGCCAAAATTACTGCTGTCAATCAAGTGGAATA
AACTTGAGGATGTTGCTCAGCTTCAGGCGCTGCTTCAGATTTGGCCTAAA
CTGCCCCCCCGGGAGGCCCTAGAGCTTCTGGATTTCAACTATCCAGACCA
GTACGTTCGAGAATATGCTGTAGGCTGCCTGCGACAGATGAGTGATGAAG
AACTTTCTCAATATCTTTTACAACTGGTGCAAGTGTTAAAATATGAGCCT
TTTCTTGATTGTGCCCTCTCTAGATTCCTATTAGAAAGGGCACTTGGTAA
TCGGAGGATAGGGCAGTTTCTATTTTGGCATCTTAGGTCAGAAGTGCACA
TTCCTGCTGTCTCAGTACAATTTGGTGTCATCCTTGAAGCATACTGCCGG
GGAAGTGTGGGGCACATGAAAGTGCTTTCTAAGCAGGTTGAAGCACTCAA
TAAGTTAAAAACTTTAAATAGTTTAATCAAACTGAATGCCGTGAAGTTAA
ACAGAGCCAAAGGGAAGGAGGCCATGCATACCTGTTTAAAACAGAGTGCT
TACCGGGAAGCCCTCTCTGACCTGCAGTCACCCCTGAACCCATGTGTTAT
CCTCTCAGAACTCTATGTTGAAAAGTGCAAATACATGGATTCCAAAATGA
AGCCTTTGTGGCTGGTATACAATAACAAGGTATTTGGTGAGGATTCAGTT
GGAGTGATTTTTAAAAATGGTGATGATTTACGACAGGATATGTTGACACT
CCAAATGTTGCGCTTGATGGATTTACTCTGGAAAGAAGCTGGTTTGGATC
TTCGGATGTTGCCTTATGGCTGTTTAGCAACAGGAGATCGCTCTGGCCTC
ATTGAAGTTGTGAGCACCTCTGAAACAATTGCTGACATTCAGCTGAACAG
TAGCAATGTGGCTGCTGCAGCAGCCTTCAACAAAGATGCCCTTCTGAACT
GGCTTAAAGAATACAACTCTGGGGATGACCTGGACCGAGCCATTGAGGAA
TTTACACTGTCCTGTGCTGGCTACTGTGTAGCTTCTTATGTCCTTGGGAT
TGGTGACAGACATAGTGACAACATCATGGTCAAAAAAACTGGCCAGCTCT
TCCACATTGACTTTGGACATATTCTTGGAAATTTCAAATCTAAGTTTGGC
ATTAAAAGGGAGCGAGTGCCTTTTATTCTTACCTATGATTTCATCCATGT
CATTCAACAAGGAAAAACAGGAAATACAGAAAAGTTTGGCCGGTTCCGCC
AGTGTTGTGAGGATGCATATCTGATTTTACGACGGCATGGGAATCTCTTC
ATCACTCTCTTTGCGCTGATGTTGACTGCAGGGCTTCCTGAACTCACATC
AGTCAAAGATATACAGTATCTTAAGGACTCTCTTGCATTAGGGAAGAGTG
AAGAAGAAGCACTCAAACAGTTTAAGCAAAAATTTGATGAGGCGCTCAGG
GAAAGCTGGACTACTAAAGTGAACTGGATGGCCCACACAGTTCGGAAAGA
CTACAGATCTTAAgcatgc

DNA sequence of insert 2 (p85 – untagged) :
gaattcGAAACCATGAGTGCTGAGGGGTACCAGTACAGAGCGCTGTATGATTATAAAAAGGAAAGAGAAGAAGATATTGACTTGCACTTGGGTGACATATTGACTGTGAATAAAGGGTCCTTAGTAGCTCTTGGATTCAGTGATGGACAGGAAGCCAGGCCTGAAGAAATTGGCTGGTTAAATGGCTATAATGAAACCACAGGGGAAAGGGGGGACTTTCCGGGAACTTACGTAGAATATATTGGAAGGAAAAAAATCTCGCCTCCCACACCAAAGCCCCGGCCACCTCGGCCTCTTCCTGTTGCACCAGGTTCTTCGAAAACTGAAGCAGATGTTGAACAACAAGCTTTGACTCTCCCGGATCTTGCAGAGCAGTTTGCCCCTCCTGACATTGCCCCGCCTCTTCTTATCAAGCTCGTGGAAGCCATTGAAAAGAAAGGTCTGGAATGTTCAACTCTATACAGAACACAGAGCTCCAGCAACCTGGCAGAATTACGACAGCTTCTTGATTGTGATACACCCTCCGTGGACTTGGAAATGATCGATGTGCACGTTTTGGCTGACGCTTTCAAACGCTATCTCCTGGACTTACCAAATCCTGTCATTCCAGCA
GCCGTTTACAGTGAAATGATTTCTTTAGCTCCAGAAGTACAAAGCTCCGA
AGAATATATTCAGCTATTGAAGAAGCTTATTAGGTCGCCTAGCATACCTC
ATCAGTATTGGCTTACGCTTCAGTATTTGTTAAAACATTTCTTCAAGCTC
TCTCAAACCTCCAGCAAAAATCTGTTGAATGCAAGAGTACTCTCTGAAAT
TTTCAGCCCTATGCTTTTCAGATTCTCAGCAGCCAGCTCTGATAATACTG
AAAACCTCATAAAAGTTATAGAAATTTTAATCTCAACTGAATGGAATGAA
CGACAGCCTGCACCAGCACTGCCTCCTAAACCACCAAAACCTACTACTGT
AGCCAACAACGGTATGAATAACAATATGTCCTTACAAGATGCTGAATGGT
ACTGGGGAGATATCTCGAGGGAAGAAGTGAATGAAAAACTTCGAGATACA
GCAGACGGGACCTTTTTGGTACGAGATGCGTCTACTAAAATGCATGGTGA
TTATACTCTTACACTAAGGAAAGGGGGAAATAACAAATTAATCAAAATAT
TTCATCGAGATGGGAAATATGGCTTCTCTGACCCATTAACCTTCAGTTCT
GTGGTTGAATTAATAAACCACTACCGGAATGAATCTCTAGCTCAGTATAA
TCCCAAATTGGATGTGAAATTACTTTATCCAGTATCCAAATACCAACAGG
ATCAAGTTGTCAAAGAAGATAATATTGAAGCTGTAGGGAAAAAATTACAT
GAATATAACACTCAGTTTCAAGAAAAAAGTCGAGAATATGATAGATTATA
TGAAGAATATACCCGCACATCCCAGGAAATCCAAATGAAAAGGACAGCTA
TTGAAGCATTTAATGAAACCATAAAAATATTTGAAGAACAGTGCCAGACC
CAAGAGCGGTACAGCAAAGAATACATAGAAAAGTTTAAACGTGAAGGCAA
TGAGAAAGAAATACAAAGGATTATGCATAATTATGATAAGTTGAAGTCTC
GAATCAGTGAAATTATTGACAGTAGAAGAAGATTGGAAGAAGACTTGAAG
AAGCAGGCAGCTGAGTATCGAGAAATTGACAAACGTATGAACAGCATTAA
ACCAGACCTTATCCAGCTGAGAAAGACGAGAGACCAATACTTGATGTGGT
TGACTCAAAAAGGTGTTCGGCAAAAGAAGTTGAACGAGTGGTTGGGCAAT
GAAAACACTGAAGACCAATATTCACTGGTGGAAGATGATGAAGATTTGCC
CCATCATGATGAGAAGACATGGAATGTTGGAAGCAGCAACCGAAACAAAG
CTGAAAACCTGTTGCGAGGGAAGCGAGATGGCACTTTTCTTGTCCGGGAG
AGCAGTAAACAGGGCTGCTATGCCTGCTCTGTAGTGGTGGACGGCGAAGT
AAAGCATTGTGTCATAAACAAAACAGCAACTGGCTATGGCTTTGCCGAGC
CCTATAACTTGTACAGCTCTCTGAAAGAACTGGTGCTACATTACCAACAC
ACCTCCCTTGTGCAGCACAACGACTCCCTCAATGTCACACTAGCCTACCC
AGTATATGCACAGCAGAGGCGATGAactagt

Amino Acid Sequence

Amino acid sequence of expressed protein 1 (6His-PI3K beta) (p10 promoter) : MHHHHHHMCFSFIMPPAMADILDIWAVDSQIASDGSIPVDFLLPTGIYIQ
LEVPREATISYIKQMLWKQVHNYPMFNLLMDIDSYMFACVNQTAVYEELE
DETRRLCDVRPFLPVLKLVTRSCDPGEKLDSKIGVLIGKGLHEFDSLKDP
EVNEFRRKMRKFSEEKILSLVGLSWMDWLKQTYPPEHEPSIPENLEDKLY
GGKLIVAVHFENCQDVFSFQVSPNMNPIKVNELAIQKRLTIHGKEDEVSP
YDYVLQVSGRVEYVFGDHPLIQFQYIRNCVMNRALPHFILVECCKIKKMY
EQEMIAIEAAINRNSSNLPLPLPPKKTRIISHVWENNNPFQIVLVKGNKL
NTEETVKVHVRAGLFHGTELLCKTIVSSEVSGKNDHIWNEPLEFDINICD
LPRMARLCFAVYAVLDKVKTKKSTKTINPSKYQTIRKAGKVHYPVAWVNT
MVFDFKGQLRTGDIILHSWSSFPDELEEMLNPMGTVQTNPYTENATALHV
KFPENKKQPYYYPPFDKIIEKAAEIASSDSANVSSRGGKKFLPVLKEILD
RDPLSQLCENEMDLIWTLRQDCREIFPQSLPKLLLSIKWNKLEDVAQLQA
LLQIWPKLPPREALELLDFNYPDQYVREYAVGCLRQMSDEELSQYLLQLV
QVLKYEPFLDCALSRFLLERALGNRRIGQFLFWHLRSEVHIPAVSVQFGV
ILEAYCRGSVGHMKVLSKQVEALNKLKTLNSLIKLNAVKLNRAKGKEAMH
TCLKQSAYREALSDLQSPLNPCVILSELYVEKCKYMDSKMKPLWLVYNNK
VFGEDSVGVIFKNGDDLRQDMLTLQMLRLMDLLWKEAGLDLRMLPYGCLA
TGDRSGLIEVVSTSETIADIQLNSSNVAAAAAFNKDALLNWLKEYNSGDD
LDRAIEEFTLSCAGYCVASYVLGIGDRHSDNIMVKKTGQLFHIDFGHILG
NFKSKFGIKRERVPFILTYDFIHVIQQGKTGNTEKFGRFRQCCEDAYLIL
RRHGNLFITLFALMLTAGLPELTSVKDIQYLKDSLALGKSEEEALKQFKQ
KFDEALRESWTTKVNWMAHTVRKDYRS*

Amino acid sequence of expressed protein 2 (p85-untagged) (Polyhedrin promoter) : MSAEGYQYRALYDYKKEREEDIDLHLGDILTVNKGSLVALGFSDGQEARP
EEIGWLNGYNETTGERGDFPGTYVEYIGRKKISPPTPKPRPPRPLPVAPG
SSKTEADVEQQALTLPDLAEQFAPPDIAPPLLIKLVEAIEKKGLECSTLY
RTQSSSNLAELRQLLDCDTPSVDLEMIDVHVLADAFKRYLLDLPNPVIPA
AVYSEMISLAPEVQSSEEYIQLLKKLIRSPSIPHQYWLTLQYLLKHFFKL
SQTSSKNLLNARVLSEIFSPMLFRFSAASSDNTENLIKVIEILISTEWNE
RQPAPALPPKPPKPTTVANNGMNNNMSLQDAEWYWGDISREEVNEKLRDT
ADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSDPLTFSS
VVELINHYRNESLAQYNPKLDVKLLYPVSKYQQDQVVKEDNIEAVGKKLH
EYNTQFQEKSREYDRLYEEYTRTSQEIQMKRTAIEAFNETIKIFEEQCQT
QERYSKEYIEKFKREGNEKEIQRIMHNYDKLKSRISEIIDSRRRLEEDLK
KQAAEYREIDKRMNSIKPDLIQLRKTRDQYLMWLTQKGVRQKKLNEWLGN
ENTEDQYSLVEDDEDLPHHDEKTWNVGSSNRNKAENLLRGKRDGTFLVRE
SSKQGCYACSVVVDGEVKHCVINKTATGYGFAEPYNLYSSLKELVLHYQH
TSLVQHNDSLNVTLAYPVYAQQRR*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5926
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus dual expression plasmid to express 6His-tagged PI3K beta and untagged p85. ; Sequence difference in PI3K beta compared with NM_006219 CDS:; a1989g (silent in R663)