cDNA Clone PI3K beta

• Plasmid: pFastBac.HTa PI3K beta (no muts)
• Parent Plasmid: pSC-A
• Expressed: 6His-PI3K beta
• Genbank: NM_006219
• Tag: 6His
• Species: Human

Sequence of Insert

GAATTCATGTGCTTCAGTTTCATAATGCCTCCTGCTATGGCAGACATCCTTGACATCTGGGCGGTGGATTCACAGATAGCATCTGATGGCTCCATACCTGTGGATTTCCTTTTGCCCACTGGGATTTATATCCAGTTGGAGGTACCTCGGGAAGCTACCATTTCTTATATTAAGCAGATGTTATGGAAGCAAGTTCACAATTACCCAATGTTCAACCTCCTTATGGATATTGACTCCTATATGTTTGCATGTGTGAATCAGACTGCTGTATATGAGGAGCTTGAAGATGAAACACGAAGACTCTGT
GATGTCAGACCTTTTCTTCCAGTTCTCAAATTAGTGACAAGAAGTTGTGA
CCCAGGGGAAAAATTAGACTCAAAAATTGGAGTCCTTATAGGAAAAGGTC
TGCATGAATTTGATTCCTTGAAGGATCCTGAAGTAAATGAATTTCGAAGA
AAAATGCGCAAATTCAGCGAGGAAAAAATCCTGTCACTTGTGGGATTGTC
TTGGATGGACTGGCTAAAACAAACATATCCACCAGAGCATGAACCATCCA
TCCCTGAAAACTTAGAAGATAAACTTTATGGGGGAAAGCTCATCGTAGCT
GTTCATTTTGAAAACTGCCAGGACGTGTTTAGCTTTCAAGTGTCTCCTAA
TATGAATCCTATCAAAGTAAATGAATTGGCAATCCAAAAACGTTTGACTA
TTCATGGGAAGGAAGATGAAGTTAGCCCCTATGATTATGTGTTGCAAGTC
AGCGGGAGAGTAGAATATGTTTTTGGTGATCATCCACTAATTCAGTTCCA
GTATATCCGGAACTGTGTGATGAACAGAGCCCTGCCCCATTTTATACTTG
TGGAATGCTGCAAGATCAAGAAAATGTATGAACAAGAAATGATTGCCATA
GAGGCTGCCATAAATCGAAATTCATCTAATCTTCCTCTTCCATTACCACC
AAAGAAAACACGAATTATTTCTCATGTTTGGGAAAATAACAACCCTTTCC
AAATTGTCTTGGTTAAGGGAAATAAACTTAACACAGAGGAAACTGTAAAA
GTTCATGTCAGGGCTGGTCTTTTTCATGGTACTGAGCTCCTGTGTAAAAC
CATCGTAAGCTCAGAGGTATCAGGGAAAAATGATCATATTTGGAATGAAC
CACTGGAATTTGATATTAATATTTGTGACTTACCAAGAATGGCTCGATTA
TGTTTTGCTGTTTATGCAGTTTTGGATAAAGTAAAAACGAAGAAATCAAC
GAAAACTATTAATCCCTCTAAATATCAGACCATCAGGAAAGCTGGAAAAG
TGCATTATCCTGTAGCGTGGGTAAATACGATGGTTTTTGACTTTAAAGGA
CAATTGAGAACTGGAGACATAATATTACACAGCTGGTCTTCATTTCCTGA
TGAACTCGAAGAAATGTTGAATCCAATGGGAACTGTTCAAACAAATCCAT
ATACTGAAAATGCAACAGCTTTGCATGTTAAATTTCCAGAGAATAAAAAA
CAACCTTATTATTACCCTCCCTTCGATAAGATTATTGAAAAGGCAGCTGA
GATTGCAAGCAGTGATAGTGCTAATGTGTCAAGTCGAGGTGGAAAAAAGT
TTCTTCCTGTATTGAAAGAAATCTTGGACAGGGATCCCTTGTCTCAACTG
TGTGAAAATGAAATGGATCTTATTTGGACTTTGCGACAAGACTGCCGAGA
GATTTTCCCACAATCACTGCCAAAATTACTGCTGTCAATCAAGTGGAATA
AACTTGAGGATGTTGCTCAGCTTCAGGCGCTGCTTCAGATTTGGCCTAAA
CTGCCCCCCCGGGAGGCCCTAGAGCTTCTGGATTTCAACTATCCAGACCA
GTACGTTCGAGAATATGCTGTAGGCTGCCTGCGACAGATGAGTGATGAAG
AACTTTCTCAATATCTTTTACAACTGGTGCAAGTGTTAAAATATGAGCCT
TTTCTTGATTGTGCCCTCTCTAGATTCCTATTAGAAAGGGCACTTGGTAA
TCGGAGGATAGGGCAGTTTCTATTTTGGCATCTTAGGTCAGAAGTGCACA
TTCCTGCTGTCTCAGTACAATTTGGTGTCATCCTTGAAGCATACTGCCGG
GGAAGTGTGGGGCACATGAAAGTGCTTTCTAAGCAGGTTGAAGCACTCAA
TAAGTTAAAAACTTTAAATAGTTTAATCAAACTGAATGCCGTGAAGTTAA
ACAGAGCCAAAGGGAAGGAGGCCATGCATACCTGTTTAAAACAGAGTGCT
TACCGGGAAGCCCTCTCTGACCTGCAGTCACCCCTGAACCCATGTGTTAT
CCTCTCAGAACTCTATGTTGAAAAGTGCAAATACATGGATTCCAAAATGA
AGCCTTTGTGGCTGGTATACAATAACAAGGTATTTGGTGAGGATTCAGTT
GGAGTGATTTTTAAAAATGGTGATGATTTACGACAGGATATGTTGACACT
CCAAATGTTGCGCTTGATGGATTTACTCTGGAAAGAAGCTGGTTTGGATC
TTCGGATGTTGCCTTATGGCTGTTTAGCAACAGGAGATCGCTCTGGCCTC
ATTGAAGTTGTGAGCACCTCTGAAACAATTGCTGACATTCAGCTGAACAG
TAGCAATGTGGCTGCTGCAGCAGCCTTCAACAAAGATGCCCTTCTGAACT
GGCTTAAAGAATACAACTCTGGGGATGACCTGGACCGAGCCATTGAGGAA
TTTACACTGTCCTGTGCTGGCTACTGTGTAGCTTCTTATGTCCTTGGGAT
TGGTGACAGACATAGTGACAACATCATGGTCAAAAAAACTGGCCAGCTCT
TCCACATTGACTTTGGACATATTCTTGGAAATTTCAAATCTAAGTTTGGC
ATTAAAAGGGAGCGAGTGCCTTTTATTCTTACCTATGATTTCATCCATGT
CATTCAACAAGGAAAAACAGGAAATACAGAAAAGTTTGGCCGGTTCCGCC
AGTGTTGTGAGGATGCATATCTGATTTTACGACGGCATGGGAATCTCTTC
ATCACTCTCTTTGCGCTGATGTTGACTGCAGGGCTTCCTGAACTCACATC
AGTCAAAGATATACAGTATCTTAAGGACTCTCTTGCATTAGGGAAGAGTG
AAGAAGAAGCACTCAAACAGTTTAAGCAAAAATTTGATGAGGCGCTCAGG
GAAAGCTGGACTACTAAAGTGAACTGGATGGCCCACACAGTTCGGAAAGA
CTACAGATCTTAAGAATTC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFMCFSFIMPPAMADILDIWAV
DSQIASDGSIPVDFLLPTGIYIQLEVPREATISYIKQMLWKQVHNYPMFN
LLMDIDSYMFACVNQTAVYEELEDETRRLCDVRPFLPVLKLVTRSCDPGE
KLDSKIGVLIGKGLHEFDSLKDPEVNEFRRKMRKFSEEKILSLVGLSWMD
WLKQTYPPEHEPSIPENLEDKLYGGKLIVAVHFENCQDVFSFQVSPNMNP
IKVNELAIQKRLTIHGKEDEVSPYDYVLQVSGRVEYVFGDHPLIQFQYIR
NCVMNRALPHFILVECCKIKKMYEQEMIAIEAAINRNSSNLPLPLPPKKT
RIISHVWENNNPFQIVLVKGNKLNTEETVKVHVRAGLFHGTELLCKTIVS
SEVSGKNDHIWNEPLEFDINICDLPRMARLCFAVYAVLDKVKTKKSTKTI
NPSKYQTIRKAGKVHYPVAWVNTMVFDFKGQLRTGDIILHSWSSFPDELE
EMLNPMGTVQTNPYTENATALHVKFPENKKQPYYYPPFDKIIEKAAEIAS
SDSANVSSRGGKKFLPVLKEILDRDPLSQLCENEMDLIWTLRQDCREIFP
QSLPKLLLSIKWNKLEDVAQLQALLQIWPKLPPREALELLDFNYPDQYVR
EYAVGCLRQMSDEELSQYLLQLVQVLKYEPFLDCALSRFLLERALGNRRI
GQFLFWHLRSEVHIPAVSVQFGVILEAYCRGSVGHMKVLSKQVEALNKLK
TLNSLIKLNAVKLNRAKGKEAMHTCLKQSAYREALSDLQSPLNPCVILSE
LYVEKCKYMDSKMKPLWLVYNNKVFGEDSVGVIFKNGDDLRQDMLTLQML
RLMDLLWKEAGLDLRMLPYGCLATGDRSGLIEVVSTSETIADIQLNSSNV
AAAAAFNKDALLNWLKEYNSGDDLDRAIEEFTLSCAGYCVASYVLGIGDR
HSDNIMVKKTGQLFHIDFGHILGNFKSKFGIKRERVPFILTYDFIHVIQQ
GKTGNTEKFGRFRQCCEDAYLILRRHGNLFITLFALMLTAGLPELTSVKD
IQYLKDSLALGKSEEEALKQFKQKFDEALRESWTTKVNWMAHTVRKDYRS
*EF

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5887
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; ; Estimated MW of expressed protein : 126475 Daltons