cDNA Clone Nur77 (mouse)

• Plasmid: pEBG6P-1 Nur77 1-260
• Parent Plasmid: pEBG6P
• Genbank: CAA42718
• Species: Mouse

Sequence of Insert

GGATCCATGCCCTGTATTCAAGCTCAATATGGAACACCAGCAACGAGCCC
AGGACCGCGTGACCACCTGACCGGTGATCCCCTGGCCCTTGAGTTCGGCA
AGCCTACCATGGACCTGGCCAGCCCCGAGACAGCACCTACCGCACCTGCT
ACACTGCCCAGCTTCAGCACCTTCATGGACGGGTACACCGGAGAGTTTGA
CACCTTCCTCTACCAGCTGCCGGGGACGACCCAGCCGTGCTCCTCAGCTT
GTTCCTCTGCCTCCTCCACGTCTTCTTCCTCATCCTCGGCCACCTCCCCC
GCTTCGGCGTCCTTCAAGTTTGAGGACTTCCAGGTGTACGGCTGCTACCC
GGGCACCCTGAGCGGCCCGTTAGATGAGACCCTATCCTCCAGCGGCTCTG
AGTACTATGGCAGTCCCTGCTCAGCCCCCTCGCCATCTACACCCAACTTC
CAGCCGTCCCAGCTTTCTCCCTGGGACGGCTCATTTGGCCACTTCTCCCC
GAGCCAGACTTATGAAGGCCTCTGGGCATGGACAGAGCAGTTGCCTAAGG
CTTCTTCAGGGCCTCCGCCACCTCCAACCTTCTTCTCCTTCAGTCCTCCC
ACTGGCCCCAGCCCCAGCCTGGCCCAGAGTTCTCTGAAATTGTTCCCACC
ACCAGCCACCCACCAGCTTGGGGAGGGGGAGAGCTATTCCATGCCAGCAG
CTTTCCCCGGCTTGGCACCCACCTCTCCGAACCGTGACACTTCCGGCATT
CTGGACGCACCCGTGACCTCCACCAAGTCCCGGAGCTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMPCIQAQYGTPAT
SPGPRDHLTGDPLALEFGKPTMDLASPETAPTAPATLPSFSTFMDGYTGE
FDTFLYQLPGTTQPCSSACSSASSTSSSSSSATSPASASFKFEDFQVYGC
YPGTLSGPLDETLSSSGSEYYGSPCSAPSPSTPNFQPSQLSPWDGSFGHF
SPSQTYEGLWAWTEQLPKASSGPPPPPTFFSFSPPTGPSPSLAQSSLKLF
PPPATHQLGEGESYSMPAAFPGLAPTSPNRDTSGILDAPVTSTKSRS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5769
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express mouse Nur77 1-260 with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 54415 Daltons