cDNA Clone I-Scei (yeast)

• Plasmid: pHR-SIN-CSGW NLS.HA.I-Scei (lent)
• Parent Plasmid: pHR SIN CSGW
• Genbank: NP_009324
• Tag: HA
• Species: Yeast

Sequence of Insert

ACTAGTGCCACCATGGGATCAAGATCGCCAAAAAAGAAGAGAAAGGTGCCGAAGAAGCATGCAGCACCACCAAAAAAAAAACGAAAAGTAGAAGACCCACGATTTATGTACCCATACGATGTTCCTGACTATGCGGGTATGAAAAACATCAAAAAAAACCAGGTAATGAACCTGGGTCCGAACTCTAAACTGCTGAAAGAATACAAATCCCAGCTGATCGAACTGAACATCGAACAGTTCGAAGCAGGTATCGGTCTGATCCTGGGTGATGCTTACATCCGTTCTCGTGATGAAGGTAAAACCTACTGTATGCAGTTCGAGTGGAAAAACAAAGCATACATGGACCACGTATGTCTGCTGTACGATCAGTGGGTACTGTCCCCGCCGCACAAAAAAGAACGTGTTAACCACCTGGGTAACCTGGTAATCACCTGGGGCGCCCAGACTTTCAAACACCAAGCTTTCAACAAACTGGCTAACCTGTTCATCGTTAACAACAAAAAAACCATCCCGAACAACCTGGTTGAAAACTACCTGACCCCGATGTCTCTGGCATACTGGTTCATGGATGATGGTGGTAAATGGGATTACAACAAAAACTCTACCAACAAA
TCGATCGTACTGAACACCCAGTCTTTCACTTTCGAAGAAGTAGAATACCT
GGTTAAGGGTCTGCGTAACAAATTCCAACTGAACTGTTACGTAAAAATCA
ACAAAAACAAACCGATCATCTACATCGATTCTATGTCTTACCTGATCTTC
TACAACCTGATCAAACCGTACCTGATCCCGCAGATGATGTACAAACTGCC
GAACACTATCTCCTCCGAAACTTTCCTGAAATAAACGCGT

Amino Acid Sequence

MGSRSPKKKRKVPKKHAAPPKKKRKVEDPRFMYPYDVPDYAGMKNIKKNQ
VMNLGPNSKLLKEYKSQLIELNIEQFEAGIGLILGDAYIRSRDEGKTYCM
QFEWKNKAYMDHVCLLYDQWVLSPPHKKERVNHLGNLVITWGAQTFKHQA
FNKLANLFIVNNKKTIPNNLVENYLTPMSLAYWFMDDGGKWDYNKNSTNK
SIVLNTQSFTFEEVEYLVKGLRNKFQLNCYVKINKNKPIIYIDSMSYLIF
YNLIKPYLIPQMMYKLPNTISSETFLK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5757
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Plasmid for lentiviral expression in mammalian cells; (SFFV promoter)