cDNA Clone Hygromycin

• Plasmid: pCMV5-Hygro (DT40) (in antisense ori?)
• Parent Plasmid: pCMV5

Sequence of Insert

GGATCCTCGAGGTGAGCCCCACGTTCTGCTTCACTCTCCCCATCTCCCCC
CCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTTTGTGC
AGCGATGGGGGCGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGG
GGCGAGGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCA
GAGCGGCGCGCTCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGCGGC
GGCCCTATAAAAAGCGAAGCGCGCGGCGGGCGGGAGTCGCTGCGTTGCCT
TCGCCCCGTGCCCCGCTCCGCGCCGCCTCGCGCCGCCCGCCCCGGCTCTG
ACTGACCGCGTTACTCCCACAGGTGAGCGGGCGGGACGGCCCTTCTCCTC
CGGGCTGTAATTAGCGCTTGGTTTAATGACGGCTCGTTTCTTTTCTGTGG
CTGCGTGAAAGCCTTAAAGGGCTCCGGGAGGGCCCTTTGTGCGGGGGGGA
GCGGCTCGGGGGGTGCGTGCGTGTGTGTGTGCGTGGGGAGCGCCGCGTGC
GGCCCGCGCTGCCCGGCGGCTGTGAGCGCTGCGGGCGCGGCGCGGGGCTT
TGTGCGCTCCGCGTGTGCGCGAGGGGAGCGCGGCCGGGGGCGGTGCCCCG
CGGTGCGGGGGGGCTGCGAGGGGAACAAAGGCTGCGTGCGGGGTGTGTGC
GTGGGGGGGTGAGCAGGGGGTGTGGGCGCGGCGGTCGGGCTGTAACCCCC
CCCTGCACCCCCCTCCCCGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCG
GGGCTCCGTGCGGGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGGTG
GCGGCAGGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCCGGGGAGG
GCTCGGGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGCGGCTGTCGAGGC
GCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCGTGCGAGAGGGCGCA
GGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCGAAATCTGGGAGGCGCC
GCCGCACCCCCTCTAGCGGGCGCGGGGCGAAGCGGTGCGGCGCCGGCAGG
AAGGAAATGGGCGGGGAGGGCCTTCGTGCGTCGCCGCGCCGCCGTCCCCT
TCTCCCTCTCCAGCCTCGGGGCTGTCCGCGGGGGGACGGCTGCCTTCGGG
GGGGACGGGGCAGGGCGGGGTTCGGCTTCTGGCGTGTGACCGGCGGCTCT
AGAGCCTCTGCTAACCATGTTCATGCCTTCTTCTTTTTCCTACAGCTCCT
GGGCAACGTGCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAGAATTAA
TTCAAGCTTGCCATGAAAAAGCCTGAACTCACCGCGACGTCTGTCGAGAA
GTTTCTGATCGAAAAGTTCGACAGCGTCTCCGACCTGATGCAGCTCTCGG
AGGGCGAAGAATCTCGTGCTTTCAGCTTCGATGTAGGAGGGCGTGGATAT
GTCCTGCGGGTAAATAGCTGCGCCGATGGTTTCTACAAAGATCGTTATGT
TTATCGGCACTTTGCATCGGCCGCGCTCCCGATTCCGGAAGTGCTTGACA
TTGGGGAATTCAGCGAGAGCCTGACCTATTGCATCTCCCGCCGTGCACAG
GGTGTCACGTTGCAAGACCTGCCTGAAACCGAACTGCCCGCTGTTCTGCA
GCCGGTCGCGGAGGCCATGGATGCGATCGCTGCGGCCGATCTTAGCCAGA
CGAGCGGGTTCGGCCCATTCGGACCGCAAGGAATCGGTCAATACACTACA
TGGCGTGATTTCATATGCGCGATTGCTGATCCCCATGTGTATCACTGGCA
AACTGTGATGGACGACACCGTCAGTGCGTCCGTCGCGCAGGCTCTCGATG
AGCTGATGCTTTGGGCCGAGGACTGCCCCGAAGTCCGGCACCTCGTGCAC
GCGGATTTCGGCTCCAACAATGTCCTGACGGACAATGGCCGCATAACAGC
GGTCATTGACTGGAGCGAGGCGATGTTCGGGGATTCCCAATACGAGGTCG
CCAACATCTTCTTCTGGAGGCCGTGGTTGGCTTGTATGGAGCAGCAGACG
CGCTACTTCGAGCGGAGGCATCCGGAGCTTGCAGGATCGCCGCGGCTCCG
GGCGTATATGCTCCGCATTGGTCTTGACCAACTCTATCAGAGCTTGGTTG
ACGGCAATTTCGATGATGCAGCTTGGGCGCAGGGTCGATGCGACGCAATC
GTCCGATCCGGAGCCGGGACTGTCGGGCGTACACAAATCGCCCGCAGAAG
CGCGGCCGTCTGGACCGATGGCTGTGTAGAAGTACTCGCCGATAGTGGAA
ACCGACGCCCCAGCACTCGTCCGAGGGCAAAGGAATAGAGTAGATGCCGA
CCGAACAAGAGCTGATTTCGAGAACGCCTCAGCCAGCAACTCGCGCGAGC
CTAGCAAGGCAAATGCGAGAGAACGGCCTTACGCTTGGTGGCACAGTTCT
CGTCCACAGTTCGCTAAGCTCGCTCGGCTGGGTCGCGGGAGGGCCGGTCG
CAGTGATTCAGGCCCTTCTGGATTGTGTTGGTCCCCAGGGCACGATTGTC
ATGCCCACGCACTCGGGTGATCTGACTGATCCCGCAGATTGGAGATCGCC
GCCCGTGCCTGCCGATTGGGTGCAGATCTGGGGAGATGGGGGAGGCTAAC
TGAAACACGGAAGGAGACAATACCGGAAGGAACCCGCGCTATGACGGCAA
TAAAAAGACAGAATAAAACGCACGGTGTTGGGTCGTTTGTTCATAAACGC
GGGGTTCGGTCCCAGGGCTGGCACTCTGTCGATACCCCACCGAGACCCCA
TTGGGGCCAATACGCCCGCGTTTCTTCCTTTTCCCCACCCCACCCCCCAA
GTTCGGGTGAAGGCCCAGGGCTCGCAGCCAACGTCGGGGCGGCAAGCCCT
GCCATAGCCACGGGCCCCGTGGGTTAGGGACGGGGTCCCCCATGGGGAAT
GGTTTATGGTTCGTGGGGGTTATTATTTTGGGCGTTGCGTGGGGTCAGGT
CCACGACTGGACTGAGCAGACAGACCCATGGTTTTTGGATGGCCTGGGCA
TGGACCGCATGTACTGGCGCGACACGAACACCGGGCGTCTGTGGCTGCCA
AACACCCCCGACCCCCAAAAACCACCGCGCGGATTTCTGGCGCCGCCGGA
CGAACTAAACCTGACTACGGCATCTCTGCCCCTTCTTCGCTGGTACGAGG
AGCGCTTTTGTTTTGTATTGGTCACCACGGCCGAGTTTCCGCGGGACCCC
GGCCAGCCAAGCTGGGCGTAATCATGGTCGACGGATCC

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5748
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Selection marker cassette for generating EWS knockout in DT40 chicken cells (http://pheasant.gsf.de/DEPARTMENT/dt40.html)