cDNA Clone EWS

• Plasmid: pCMV5 DT40 NEO EWS 5′Arm/EWS 3′Arm
• Parent Plasmid: pCMV5
• Species: Chicken

Sequence of Insert

DNA sequence of EWS5’Arm insert :
gaattcatgcggccgcAGCGGGATGTTAAATCACTGCCTTTTCTTTCTCTGGTGCAAACGCTTCCTGCCGAGCTGCCTGCACAAGGGAACTGCTCCATCCAGAGCTGAAGCATTACAATTCAGGATGATTTTCTCCTCCTTTTTATTCTAATGCCTGTGCTGAGCCTGCTGCTAGCAAACCCACTCATCTGtGCTGCCCATGGTGGCACCATGAGGGTCCCTTTCTGGAGGACGACTGCCTCTGTGTGCTGCTCCCCAGGGCTGGGCTCAGGGCTGTGGTTTCCGTGGTATaTGTGTCAGACCATACTGCAAGCAGCGGGTTTTTTTTAGTAAGTGGTCAGGCTTCAGCCACTTAAAGTAAGGATTCCTTCAGCTTTGTAAAGTAGAAGCACTGTGGTGGCTTACCTCGAAGTGTTTGAGCTGTTTAATTTTGTCTGAGGCACTAAAAGCCACGCTCAGTCGACtgTGTTTGATTTCACCTCAGTCCCCCTCTCCAGTAACACTCAGAGCAGGGCTTTGCTTTGCACAGAACCTCTGAGCAGCCAGGCACAGCTGAACAACCAAGGAAACTGCCCGCCAGCGATTCCTGCCCATGTCTCCTGGTGGGAAGGGAGCTTCCTTGCTCCCAGCTTGGCAGGAGCACGATGGGAAGCTGCTCCACAGTGTTCAGGAGGGTGGTGATGTGATAAGCGTGCAGGGCAGCAGGCACAGGGAACAACCTGGGGACTGACAcTTGcaGATGGAGTAAAGGGAAAGACTTCTTCCTGCTCAGAGCCCCTTGTCAGCATGCTaGGGCCAACTGGAACGCTCAGGAGT
AACACACAGGGCAGGATTTTGGCTTTGTTTGCTGCACCCACTTCTCTTCT
CACTTCCCTTCGTCTCTGCTTTCTGGTTTGAGAACCCTGATTTTTCCAGC
AGGTTACCTGCAGAGGTTGTGCTTCAGGAGGAGCACAtGCTTCgTTCTGC
TGGAAGTGCTTTCATTTTGGAAAGGGAGGAATCCACATaAAAAGCCTACA
GCACAGTTTGCCTGATGTGCTGTGTaAAGAGTAGGTCACAAGTGTCCTGT
GTGTAACAAAAATATTGAGTGATGGAGAAGCTGTTACCTTAATTGATGCA
ATTAAGATCACTTCAGTAAGACTGAAACCAGTTGACTGCTTTTTAGCAaT
GGATGCTTTTgTaCTTTAGTAAGAGAGCTTTTGCTAACAAAATGTTTAAT
TGGTAGCTTTTCATGCAAATCTTTGTTTCGTAGTTGGAAAACCAGCTTGT
GTGGCTCTTAACTATAAAAAAGGCACTGTGGTAATACTTGGTTTGTTGTT
CATCTTTGTTGACAAGCCAAATGGTCAGCTGAATTACCTAGCAACAGCTG
CTGGGAACACTGATCTCAGTGGAGCTGGTTTGGGGTCTGAGATACAGTAA
AACTGTTCAGAAATAAATGTGTGAGCTCTGAGCTTTTGAGCTGAAGCAGA
CTGAAATTCTAAGAGAAGTGAGGGTACATAAAGCATCTTACAGACCGACA
CACgGGGCAGCTCCTGACCTTTCTCTTAAGCTAAAGCAAACGTAAAGTGC
TTTCCTTCAGTTTCTAGAATTGCTGTTTAAAGTAATGCTTACAAAGGGCT
CAGAAAGACTTCTcTGCCCGCCTGGTGCACTTCTGTGCTTTCGTTTCTCA
AAAAAGCTGCCAGGATGGGACTGTGCTTTTCCAATTAATTGCATTTCTTT
GACTGTGAAGCCAGGAGGAGCTTTGTGTAGCCCACTGGACAAAAGAAGGC
GCTGCATGCCTCAGCCTGCCCTGCTGCGCTGCATTAGCTGGGAGCCTCAG
GTGCTGTGAACAAACAGTCCTTGGATGCTGGTgGCAGGCTGGCTTCCAGA
AAGCTCCTCGGGGTGTGcGTTGGGGATGGAGAGGGTATTTCTGGGGCAGG
CTGAGCTTTCCCAGGGTGGGGGCTTTGCAAGGGAGGCGAAGGGATGCTGT
GGGTTCGTTAGACACACTCAGGCCACCCGTGAGGTTTCTGCAGCAGTGttaattaagcgaattc

DNA sequence of EWS3’Arm insert : ggatccCCAGTCCCACAATATTTTCAGAGCTGGAAGAAGCTGTGTAGGTGCTTCTGTTCTGGCACAGTGAGTCAGCGCTGCTACTGGGAAATAGAGAGAGCAACCAAGCAGCACAAATGGGCATTTGACTCATGATCTGGACCAGAAAATGGACCAGTCTGCTTGAGTCACGGGGTGGTTAGGATGATGCTATGCCCTGTGATTTCTTAAGTGGCTGCACATGCTGAAGACAACTTCCTTTTTAAATCTCTTGTGTCTGGGCATTGTCgGTAGCATGTTCAACTTAATTGTTTGCTCAAGATATTA
CaGGcTTAGAACGTGGAATGGAAACtTAGGAGCTAGTTAAAACTGCTTTA
TTCATTTACCTGATGTTTGCAGTGACTGCCCCTGAATAACAAGGCCAGAG
CATGACACAGCCCTGGCTGTATCTCTCAGATTACACTTAAGTATGAATCC
TGGTGTCATTTCAAATATTCTTCCCAGCTTGTACAATAAAAGTACTTCAG
TTCCTTTTTTCATTCAGCTCTGATAAACACTTTGAAATTCTAAGCAGAAG
GGGACTAATCTAAAAAGAAGGGGACATGCAAACTGCATGCAGAGAGTTCT
CTTTATTTTCAGGCCTTGAGAGTTAATGCTAAACATCCTCTCCCTTTCAT
CAGATAACGTATGTGGGGCAATATCTGCTTGCACTGGATTCTAAACAGAA
CAATGGTGCCTTTAACTGGCAGCATTTCTCgCTGCTTTGGAGCTAATCGT
TTACAAGCATTGTGCAAAACAGAGCAGAGGGGCAGCGCTGCTGACCGACT
TTCCTGGCAcTTTCACATCTTCTGTTCCTAGAGCCAAAGTCTGAACCCCT
CGCTGTAGGTTTCATAATGCAGCTGGCAAATAGAAGCTGTGGTGCTCTCC
AAGTGCTCTCAGTTCAGAATGTTGTTTCTTGGTGGCAGCGGATTGTGCAG
TGGGTAATAGTGCTTAGCTTGTGTGTACTAGCTGtTATTGAGTGACCAGC
ACTGATGTGCTGATTTGTAACCCTTCCCAAAGAAAGTGCCATCCTGCCAC
AGGGGAGCTGTATACCTCTGGTGCTTCTACCTGTGGAGGTCTGTGCTTGT
GTCTCCTTATTGTTCATTATTACTTTCAGACCCCAGGATGGCAGCAAACC
AGCAGAGACCAGCCAGCCACCATCAAGTACAACAGGCTACAACCAGCCCA
GCCTAGGGTATGGGCAGAGCAACTACAGCTACCCCCAGGTGCCTGCCAGC
TACCCGATGCAGCCGGTTACTGCGCCTCCCTCCTATCCTCCTACCAGGTA
ATTCTTTCAGAAAGTGAAGCCTCTTGCAACTAGCGTGGGAGCTTTGAGAT
TAACTGGGACAAAAGGATTTTTTTCTcAAGGGTTTTTAGGTGAATGAGTG
TAGAAAGAGATTTAGTATCCTGGGAGGAGGAGTGTGGTTCTGGTTGGAGT
GGTGCTAGCTCTAGCGATAAGACAAGGGTTGAATCATAGAATCATGGGAT
CATTAAGGTTGGAAAAGATCTCTACCATCATAGAGACCAACTGTCAGTCT
gTTACCACCATGACCACTAAACCACaTCCCTCAGTGCCGCATCTCCACAG
TTCTGaAACACCTCCAGGGATGGTGACTCCACCACCTCTCTGGGCAGTCT
GTGCCAATGAGTCACCACTCTTCCAGAGAAGAAATGTTTCTTAATATCCA
GCCTGAATCTCCAGGGAGACCTGAGaGCAGCCTTTCAGTTGCTAAAGGTG
GGGTGgGGGAAGGGGAGGGAGGAGCATAAGAAAAAAGGGACAGACTTTtt
aacaggatCTGTGGTGAtAGGACAAGAGGAAGTGGTTTCAAACTAAAAGA
GGgGAGATTTAGATTGAATATAAGAAAAAgGTTTTTTACTATTAGATaGT
GGAGGACTGGCACAGGTTGCCCAGAGgcggccgcatggatcc

DNA sequence of Neomycin insert : ggatccTCGAGGTGAGCCCCACGTTCTGCTTCACTCTCCCCATCTCCCCC
CCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTTTGTGC
AGCGATGGGGGCGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGG
GGCGAGGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCA
GAGCGGCGCGCTCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGCGGC
GGCCCTATAAAAAGCGAAGCGCGCGGCGGGCGGGAGTCGCTGCGTTGCCT
TCGCCCCGTGCCCCGCTCCGCGCCGCCTCGCGCCGCCCGCCCCGGCTCTG
ACTGACCGCGTTACTCCCACAGGTGAGCGGGCGGGACGGCCCTTCTCCTC
CGGGCTGTAATTAGCGCTTGGTTTAATGACGGCTCGTTTCTTTTCTGTGG
CTGCGTGAAAGCCTTAAAGGGCTCCGGGAGGGCCCTTTGTGCGGGGGGGA
GCGGCTCGGGGGGTGCGTGCGTGTGTGTGTGCGTGGGGAGCGCCGCGTGC
GGCCCGCGCTGCCCGGCGGCTGTGAGCGCTGCGGGCGCGGCGCGGGGCTT
TGTGCGCTCCGCGTGTGCGCGAGGGGAGCGCGGCCGGGGGCGGTGCCCCG
CGGTGCGGGGGGGCTGCGAGGGGAACAAAGGCTGCGTGCGGGGTGTGTGC
GTGGGGGGGTGAGCAGGGGGTGTGGGCGCGGCGGTCGGGCTGTAACCCCC
CCCTGCACCCCCCTCCCCGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCG
GGGCTCCGTGCGGGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGGTG
GCGGCAGGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCCGGGGAGG
GCTCGGGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGCGGCTGTCGAGGC
GCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCGTGCGAGAGGGCGCA
GGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCGAAATCTGGGAGGCGCC
GCCGCACCCCCTCTAGCGGGCGCGGGGCGAAGCGGTGCGGCGCCGGCAGG
AAGGAAATGGGCGGGGAGGGCCTTCGTGCGTCGCCGCGCCGCCGTCCCCT
TCTCCCTCTCCAGCCTCGGGGCTGTCCGCGGGGGGACGGCTGCCTTCGGG
GGGGACGGGGCAGGGCGGGGTTCGGCTTCTGGCGTGTGACCGGCGGCTCT
AGAGCCTCTGCTAACCATGTTCATGCCTTCTTCTTTTTCCTACAGCTCCT
GGGCAACGTGCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAGAATTAA
TTCAAGCTAGCTTGGCGCGTGTGGCCTCGAACACCGAGCGACCCTGCAGC
CAATATGGGATCGGCCATTGAACAAGATGGATTGCACGCAGGTTCTCCGG
CCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATC
GGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGT
TCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACG
AGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCT
GTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGA
AGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAG
TATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCT
ACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTAC
TCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATC
AGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCC
GACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATAT
CATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGG
GTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCT
GAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTAT
CGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGT
TCTTCTGAGGGGATCGGCAATAAAAAGACAGAATAAAACGCACGGGTGTT
GGGTCGTTTGTTCggatcc

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5739
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Neo selection marker cassette obtained from Jean-Marie Buerstedde Department of Cellular Immunology; Heinrich-Pette-Institut for Experimental Virology and Immunology Hamburg, Germany.