cDNA Clone PTIP

• Plasmid: pHR-SIN-CSGW FLAG.hPTIP (lent)
• Parent Plasmid: pHR SIN CSGW
• Expressed: FLAG-PTIP
• Tag: FLAG
• Species: Human

Sequence of Insert

ACTAGTGCCACCATGGACTACAAGGACGACGATGACAAGTCGGACCAGGCGCCCAAAGTTCCTGAGGAGATGTTCAGGGAGGTCAAGTATTACGCGGTGGGCGACATCGACCCGCAGGTTATTCAGCTTCTCAAGGCTGGAAAAGCGAAGGAAGTTTCCTACAATGCACTAGCCTCACACATAATCTCAGAGGATGGGGACAATCCAGAGGTGGGAGAAGCTCGGGAAGTCTTTGACTTACCTGTTGTAAAGCCTTCTTGGGTGATTCTGTCCGTTCAGTGTGGAACTCTTCTGCCAGTAAATGGTTTTTCTCCAGAATCATGTCAGATTTTTTTTGGAATCACTGCCTGCCTTTCTCAGGTGTCATCTGAAGACAGAAGTGCCCTGTGGGCTTTGGTTACGTTCTATGGGGGAGATTGCCAGCTAACCCTCAATAAGAAATGCACGCATTTGATTGTTCCAGAGCCAAAGGGGGAGAAATACGAATGTGCTTTAAAGCGAGCAAGTATTAAAATTGTGACTCCTGACTGGGTTCTGGATTGCGTATCAGAGAAAACCAAAAAGGACGAAGCATTTTATCATCCTCGTCTGATTATTTATGAAGAGGAAGAAGAGGAAGAGGAAGAGGAGGAGGAAGTAGAAAATGAGGAACAAGATTCTCAGAATGAGGGTAGTACAGATGAGAAGTCAAGCCCTGCCAGCTCTCAAGAAGGGTCTCCTTCAGGTGACCAGCAGTTTTCACCTAAATCCAACACTGAAAAATCTAAAGGGGAATTAATGTTTGATGATTCTTCAGATTCATCACCGGAAAAACAGGAGAGAAATTTAAACTGGACCCCGGCCGAAGTCCCACAGTTAGCTGCAGCAAAACGCAGGCTGCCTCAGGGAAAGGAGCCTGGGTTGATTAACTTGTGTGCCAATGTCCCACCCGTCCCAGGTAACATTTTGCCCCCTGAGGTCCGGGGTAATTTAATGGCTGCTGGACAAAACCTCCAAAGTTCTGAAAGATCAGAAATGATAGCTACCTGGAGTCCAGCTGTACGGACACTGAGGAATATTACTAATAATGCTGACATTCAGCAGATGAACCGGCCATCAAATGTAGCACATATCCTACAGACTCTCTCAGCACCTACGAAAAATTTAGAACAGCAGGTGAATCACAGCCAGCAGGGACATACAAATGCCAATGCAGTGCTGTTTAGCCAAGTGAAAGTGACTCCAGAGACACACATGCTACAGCAGCAGCAGCAGGCCCAGCAGCAGCAGCAGCAGCACCCGGTTTTACACCTTCAGCCCCAGCAGATAATGCAGCTCCAGCAGCAGCAGCAGCAGCAGATCTCTCAGCAACCTTACCCCCAGCAGCCGCCGCATCCATTTTCACAGCAACAGCAGCAGCAGCAGCAAGCCCATCCGCATCAGTTTTCACAGCAACAGCTACAGTTTCCACAGCAACAGTTGCATCCTCCACAGCAGCTGCATCGCCCTCAGCAGCAGCTCCAGCCCTTTCAGCAGCAGCATGCCCTGCAGCAGCAGTTCCATCAGCTGCAGCAGCACCAGCTCCAGCAGCAGCAGCTCGCCCAGCTCCAGCAGCAGCACAGCCTGCTCCAGCAGCAGCAGCAACAGCAGATTCAGCAGCAGCAGCTCCAGCGCATGCACCAGCAGCAGCAGCAGCAGCAGATGCAAAGTCAGACAGCGCCACACTTGAGTCAGACGTCACAGGCGCTGCAGCATCAGGTTCCACCTCAGCAGCCCCCGCAGCAGCAGCAGCAACAGCAGCCACCACCATCGCCTCAGCAGCATCAGCTTTTTGGACATGATCCAGCAGTGGAGATT
CCAGAAGAAGGCTTCTTATTGGGATGTGTGTTTGCAATTGCGGATTATCC
AGAGCAGATGTCTGATAAGCAACTGCTGGCCACCTGGAAAAGGATAATCC
AGGCACATGGCGGCACTGTTGACCCCACCTTCACGAGTCGATGCACGCAC
CTTCTCTGTGAGAGTCAAGTCAGCAGCGCGTATGCACAGGCAATAAGAGA
AAGAAAGAGATGTGTTACTGCACACTGGTTAAACACAGTCTTAAAGAAGA
AGAAAATGGTACCGCCGCACCGAGCCCTTCACTTCCCAGTGGCCTTCCCA
CCAGGAGGAAAGCCATGTTCACAGCATATTATTTCTGTGACTGGATTTGT
TGATAGTGACAGAGATGACCTAAAATTAATGGCTTATTTGGCAGGTGCCA
AATATACGGGTTATCTATGCCGCAGCAACACAGTCCTCATCTGTAAAGAA
CCAACTGGTTTAAAGTATGAAAAAGCCAAAGAGTGGAGGATACCCTGTGT
CAACGCCCAGTGGCTTGGCGACATTCTTCTGGGAAACTTTGAGGCACTGA
GGCAGATTCAGTATAGTCGCTACACGGCATTCAGTCTGCAGGATCCATTT
GCCCCTACCCAGCATTTAGTTTTAAATCTTTTAGATGCTTGGAGAGTTCC
CTTAAAAGTGTCTGCAGAGTTGTTGATGAGTATAAGACTACCTCCCAAAC
TGAAACAGAATGAAGTAGCTAATGTCCAGCCTTCTTCCAAAAGAGCCAGA
ATTGAAGACGTACCACCTCCCACTAAAAAGCTAACTCCAGAATTGACCCC
TTTTGTGCTTTTCACTGGATTCGAGCCTGTCCAGGTTCAACAGTATATTA
AGAAGCTCTACATTCTTGGTGGAGAGGTTGCGGAGTCTGCACAGAAGTGC
ACACACCTCATTGCCAGCAAGGTGACTCGCACCGTGAAGTTCCTGACGGC
GATTTCTGTCGTGAAGCACATAGTGACGCCAGAGTGGCTGGAAGAATGCT
TCAGGTGTCAGAAGTTCATTGATGAGCAGAACTACATTCTCCGAGATGCT
GAGGCAGAAGTACTTTTCTCTTTCAGCTTGGAAGAATCCTTAAAACGGGC
ACACGTTTCTCCACTCTTTAAGGCAAAATATTTTTACATCACACCTGGAA
TCTGCCCAAGTCTTTCCACTATGAAGGCAATCGTAGAGTGTGCAGGAGGA
AAGGTGTTATCCAAGCAGCCATCTTTCCGGAAGCTCATGGAGCACAAGCA
GAACTCGAGTTTGTCGGAAATAATTTTAATATCCTGTGAAAATGACCTTC
ATTTATGCCGAGAATATTTTGCCAGAGGCATAGATGTTCACAATGCAGAG
TTCGTTCTGACTGGAGTGCTCACTCAAACGCTGGACTATGAATCATATAA
GTTTAACTGAAGCGCT

Amino Acid Sequence

MDYKDDDDKSDQAPKVPEEMFREVKYYAVGDIDPQVIQLLKAGKAKEVSY
NALASHIISEDGDNPEVGEAREVFDLPVVKPSWVILSVQCGTLLPVNGFS
PESCQIFFGITACLSQVSSEDRSALWALVTFYGGDCQLTLNKKCTHLIVP
EPKGEKYECALKRASIKIVTPDWVLDCVSEKTKKDEAFYHPRLIIYEEEE
EEEEEEEEVENEEQDSQNEGSTDEKSSPASSQEGSPSGDQQFSPKSNTEK
SKGELMFDDSSDSSPEKQERNLNWTPAEVPQLAAAKRRLPQGKEPGLINL
CANVPPVPGNILPPEVRGNLMAAGQNLQSSERSEMIATWSPAVRTLRNIT
NNADIQQMNRPSNVAHILQTLSAPTKNLEQQVNHSQQGHTNANAVLFSQV
KVTPETHMLQQQQQAQQQQQQHPVLHLQPQQIMQLQQQQQQQISQQPYPQ
QPPHPFSQQQQQQQQAHPHQFSQQQLQFPQQQLHPPQQLHRPQQQLQPFQ
QQHALQQQFHQLQQHQLQQQQLAQLQQQHSLLQQQQQQQIQQQQLQRMHQ
QQQQQQMQSQTAPHLSQTSQALQHQVPPQQPPQQQQQQQPPPSPQQHQLF
GHDPAVEIPEEGFLLGCVFAIADYPEQMSDKQLLATWKRIIQAHGGTVDP
TFTSRCTHLLCESQVSSAYAQAIRERKRCVTAHWLNTVLKKKKMVPPHRA
LHFPVAFPPGGKPCSQHIISVTGFVDSDRDDLKLMAYLAGAKYTGYLCRS
NTVLICKEPTGLKYEKAKEWRIPCVNAQWLGDILLGNFEALRQIQYSRYT
AFSLQDPFAPTQHLVLNLLDAWRVPLKVSAELLMSIRLPPKLKQNEVANV
QPSSKRARIEDVPPPTKKLTPELTPFVLFTGFEPVQVQQYIKKLYILGGE
VAESAQKCTHLIASKVTRTVKFLTAISVVKHIVTPEWLEECFRCQKFIDE
QNYILRDAEAEVLFSFSLEESLKRAHVSPLFKAKYFYITPGICPSLSTMK
AIVECAGGKVLSKQPSFRKLMEHKQNSSLSEIILISCENDLHLCREYFAR
GIDVHNAEFVLTGVLTQTLDYESYKFN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5678
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Plasmid for lentiviral expression in mammalian cells; (SFFV promoter)