cDNA Clone PTIP

• Plasmid: pEBG6P-1 hPTIP I600-N1069(end)
• Parent Plasmid: pEBG6P
• Expressed: GST-PTIP I600-N1069(end)
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATTCCAGAAGAAGGCTTCTTATTGGGATGTGTGTTTGCAATTGCGGATTATCCAGAGCAGATGTCTGATAAGCAACTGCTGGCCACCTGGAAAAGGATAATCCAGGCACATGGCGGCACTGTTGACCCCACCTTCACGAGTCGATGCACGCACCTTCTCTGTGAGAGTCAAGTCAGCAGCGCGTATGCACAGGCAATAAGAGAAAGAAAGAGATGTGTTACTGCACACTGGTTAAACACAGTCTTAAAGAAGAAGAAAATGGTACCGCCGCACCGAGCCCTTCACTTCCCAGTGGCCTTCCCACCAGGAGGAAAGCCATGTTCACAGCATATTATTTCTGTGACTGGATTTGTTGATAGTGACAGAGATGACCTAAAATTAATGGCTTATTTGGCAGGTGCCAAATATACGGGTTATCTATGCCGCAGCAACACAGTCCTCATCTGTAAA
GAACCAACTGGTTTAAAGTATGAAAAAGCCAAAGAGTGGAGGATACCCTG
TGTCAACGCCCAGTGGCTTGGCGACATTCTTCTGGGAAACTTTGAGGCAC
TGAGGCAGATTCAGTATAGTCGCTACACGGCATTCAGTCTGCAGGATCCA
TTTGCCCCTACCCAGCATTTAGTTTTAAATCTTTTAGATGCTTGGAGAGT
TCCCTTAAAAGTGTCTGCAGAGTTGTTGATGAGTATAAGACTACCTCCCA
AACTGAAACAGAATGAAGTAGCTAATGTCCAGCCTTCTTCCAAAAGAGCC
AGAATTGAAGACGTACCACCTCCCACTAAAAAGCTAACTCCAGAATTGAC
CCCTTTTGTGCTTTTCACTGGATTCGAGCCTGTCCAGGTTCAACAGTATA
TTAAGAAGCTCTACATTCTTGGTGGAGAGGTTGCGGAGTCTGCACAGAAG
TGCACACACCTCATTGCCAGCAAGGTGACTCGCACCGTGAAGTTCCTGAC
GGCGATTTCTGTCGTGAAGCACATAGTGACGCCAGAGTGGCTGGAAGAAT
GCTTCAGGTGTCAGAAGTTCATTGATGAGCAGAACTACATTCTCCGAGAT
GCTGAGGCAGAAGTACTTTTCTCTTTCAGCTTGGAAGAATCCTTAAAACG
GGCACACGTTTCTCCACTCTTTAAGGCAAAATATTTTTACATCACACCTG
GAATCTGCCCAAGTCTTTCCACTATGAAGGCAATCGTAGAGTGTGCAGGA
GGAAAGGTGTTATCCAAGCAGCCATCTTTCCGGAAGCTCATGGAGCACAA
GCAGAACTCGAGTTTGTCGGAAATAATTTTAATATCCTGTGAAAATGACC
TTCATTTATGCCGAGAATATTTTGCCAGAGGCATAGATGTTCACAATGCA
GAGTTCGTTCTGACTGGAGTGCTCACTCAAACGCTGGACTATGAATCATA
TAAGTTTAACTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAIPEE
GFLLGCVFAIADYPEQMSDKQLLATWKRIIQAHGGTVDPTFTSRCTHLLC
ESQVSSAYAQAIRERKRCVTAHWLNTVLKKKKMVPPHRALHFPVAFPPGG
KPCSQHIISVTGFVDSDRDDLKLMAYLAGAKYTGYLCRSNTVLICKEPTG
LKYEKAKEWRIPCVNAQWLGDILLGNFEALRQIQYSRYTAFSLQDPFAPT
QHLVLNLLDAWRVPLKVSAELLMSIRLPPKLKQNEVANVQPSSKRARIED
VPPPTKKLTPELTPFVLFTGFEPVQVQQYIKKLYILGGEVAESAQKCTHL
IASKVTRTVKFLTAISVVKHIVTPEWLEECFRCQKFIDEQNYILRDAEAE
VLFSFSLEESLKRAHVSPLFKAKYFYITPGICPSLSTMKAIVECAGGKVL
SKQPSFRKLMEHKQNSSLSEIILISCENDLHLCREYFARGIDVHNAEFVL
TGVLTQTLDYESYKFN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5633
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express hPTIP I600-N1069(end) with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 81451 Daltons