cDNA Clone MEKK3 (mouse)

• Synonyms: Map3k3
• Plasmid: pEBG6P-1 (mouse) MEKK3 delta 1-11
• Parent Plasmid: pEBG6P
• Genbank: NM_011947
• Species: Mouse

Sequence of Insert

GGTACCAAGGACCTGGTGGCCCTCCAGATGAGCCGACGAACCCGGTTGTCTGGATATGAGACCATGAAGAATAAGGACACAGGTCACCCAAACAGGCAGAGTGACGTCAGAATCAAGTTTGAACACAATGGGGAGAGACGAATTATAGCATTCAGCCGGCCTGTGAGATACGAAGATGTGGAGCACAAGGTGACAACAGTCTTTGGGCAGCCTCTTGATTTGCATTATATGAATAATGAGCTCTCCATCCTGTTGAAAAACCAAGATGATCTCGATAAAGCCATTGACATTTTGGATAGAAGCTCA
AGTATGAAAAGCCTTAGGATACTACTGTTATCCCAAGACAGAAACCATAC
TAGTTCCTCTCCCCACTCTGGAGTGTCCAGGCAGGTTCGGATCAAGCCTT
CCCAGTCTGCAGGGGATATAAATACCATCTACCAAGCTCCTGAGCCCAGA
AGCAGGCACCTGTCTGTCAGCTCCCAGAACCCTGGCCGAAGCTCTCCTCC
CCCGGGATATGTACCTGAGCGACAACAGCACATTGCCCGGCAAGGATCCT
ATACGAGCATCAACAGCGAAGGTGAATTCATCCCAGAGACCAGCGAACAG
TGTATGCTAGATCCCCTCAGCAGTGCCGAAAATTCCTTGTCAGGAAGCTG
CCAATCCTTGGACAGGTCAGCAGACAGCCCATCCTTCAGGAAATCACAAA
TGTCCCGAGCCCGGAGCTTCCCAGACAACAGAAAGGAATGCTCAGATCGG
GAGACCCAGCTCTATGATAAAGGTGTCAAAGGTGGAACCTATCCCAGGCG
CTACCATGTGTCTGTGCATCACAAAGACTACAATGATGGCAGAAGAACAT
TTCCCCGAATACGACGGCATCAAGGCAACCTATTCACTCTGGTGCCCTCA
AGTCGCTCCTTGAGCACAAATGGCGAGAACATGGGTGTAGCTGTGCAATA
CCTGGACCCCCGTGGGCGCCTACGGAGTGCAGACAGTGAGAATGCCCTCA
CTGTGCAGGAAAGGAATGTGCCAACCAAATCTCCTAGTGCTCCCATCAAT
TGGCGTCGGGGGAAGCTCCTGGGTCAAGGTGCCTTCGGCAGGGTCTACTT
GTGCTATGATGTGGACACAGGACGTGAACTTGCTTCTAAGCAGGTCCAGT
TTGACCCAGATAGTCCTGAGACAAGCAAGGAGGTGAGTGCTCTGGAGTGT
GAGATCCAGTTGCTGAAGAACCTGCAGCATGAGCGCATTGTGCAGTACTA
CGGCTGCCTGCGGGACCGTGCTGAGAAGATCCTCACCATCTTTATGGAGT
ATATGCCAGGGGGCTCTGTAAAAGACCAGTTGAAGGCCTACGGAGCTCTG
ACAGAGAGTGTGACCCGCAAGTACACCCGGCAGATTCTGGAGGGCATGTC
ATACCTGCACAGCAACATGATTGTGCATCGGGACATCAAGGGAGCCAATA
TCCTCCGAGACTCAGCTGGGAATGTGAAGCTTGGGGATTTTGGGGCCAGC
AAACGCCTACAGACCATCTGCATGTCAGGGACAGGCATTCGCTCTGTCAC
TGGCACACCCTACTGGATGAGTCCTGAAGTCATCAGTGGCGAGGGCTATG
GAAGAAAGGCAGACGTGTGGAGCCTGGGCTGTACTGTGGTGGAAATGCTG
ACAGAGAAACCACCTTGGGCAGAGTATGAAGCTATGGCTGCCATTTTCAA
GATTGCCACCCAGCCTACCAATCCTCAGCTGCCCTCTCACATCTCAGAAC
ACGGCAGGGACTTCCTGAGGCGCATATTTGTGGAAGCTCGTCAGAGACCC
TCAGCTGAGGAGCTGCTCACACACCACTTTGCACAGCTAGTGTACTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTKDLVALQMS
RRTRLSGYETMKNKDTGHPNRQSDVRIKFEHNGERRIIAFSRPVRYEDVE
HKVTTVFGQPLDLHYMNNELSILLKNQDDLDKAIDILDRSSSMKSLRILL
LSQDRNHTSSSPHSGVSRQVRIKPSQSAGDINTIYQAPEPRSRHLSVSSQ
NPGRSSPPPGYVPERQQHIARQGSYTSINSEGEFIPETSEQCMLDPLSSA
ENSLSGSCQSLDRSADSPSFRKSQMSRARSFPDNRKECSDRETQLYDKGV
KGGTYPRRYHVSVHHKDYNDGRRTFPRIRRHQGNLFTLVPSSRSLSTNGE
NMGVAVQYLDPRGRLRSADSENALTVQERNVPTKSPSAPINWRRGKLLGQ
GAFGRVYLCYDVDTGRELASKQVQFDPDSPETSKEVSALECEIQLLKNLQ
HERIVQYYGCLRDRAEKILTIFMEYMPGGSVKDQLKAYGALTESVTRKYT
RQILEGMSYLHSNMIVHRDIKGANILRDSAGNVKLGDFGASKRLQTICMS
GTGIRSVTGTPYWMSPEVISGEGYGRKADVWSLGCTVVEMLTEKPPWAEY
EAMAAIFKIATQPTNPQLPSHISEHGRDFLRRIFVEARQRPSAEELLTHH
FAQLVY*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5628
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 97274 Daltons