cDNA Clone MEF2a

• Plasmid: pCMV5-FLAG MEF2A
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG-MEF2A
• Genbank: NM_005587*
• Tag: FLAG
• Species: Human

Sequence of Insert

gaattcATGGGGCGGAAGAAAATACAAATCACACGCATAATGGATGAAAGGAACCGACAGGTCACTTTTACAAAGAGAAAGTTTGGATTAATGAAGAAAGCCTATGAACTTAGTGTGCTCTGTGACTGTGAAATAGCACTCATCATTTTCAACAGCTCTAACAAACTGTTTCAATATGCTAGCACTGATATGGACAAAGTTCTTCTCAAGTATACAGAATATAATGAACCTCATGAAAGCAGAACCAACTCGGATATTGTTGAGGCTCTGAACAAGAAGGAACACAGAGGGTGCGACAGCCCAGAC
CCTGATACTTCATATGTGCTAACTCCACATACAGAAGAAAAATATAAAAA
AATTAATGAGGAATTTGATAATATGATGCGGAATCATAAAATCGCACCTG
GTCTGCCACCTCAGAACTTTTCAATGTCTGTCACAGTTCCAGTGACCAGC
CCCAATGCTTTGTCCTACACTAACCCAGGGAGTTCACTGGTGTCCCCATC
TTTGGCAGCCAGCTCAACGTTAACAGATTCAAGCATGCTCTCTCCACCTC
AAACCACATTACATAGAAATGTGTCTCCTGGAGCTCCTCAGAGACCACCA
AGTACTGGCAATGCAGGTGGGATGTTGAGCACTACAGACCTCACAGTGCC
AAATGGAGCTGGAAGCAGTCCAGTGGGGAATGGATTTGTAAACTCAAGAG
CTTCTCCAAATTTGATTGGAGCTACTGGTGCAAATAGCTTAGGCAAAGTC
ATGCCTACAAAGTCTCCCCCTCCACCAGGTGGTGGTAATCTTGGAATGAA
CAGTAGGAAACCAGATCTTCGAGTTGTCATCCCCCCTTCAAGCAAGGGCA
TGATGCCTCCACTAAATACCCAAAGGATCAGTAGTTCTCAAGCCACTCAA
CCTCTTGCTACCCCAGTCGTGTCTGTGACAACCCCAAGCTTGCCTCCGCA
AGGACTTGTGTACTCAGCAATGCCGACTGCCTACAACACTGATTATTCAC
TGACCAGCGCTGACCTGTCAGCCCTTCAAGGCTTCAACTCGCCAGGAATG
CTGTCGCTGGGACAGGTGTCGGCCTGGCAGCAGCACCACCTAGGACAAGC
AGCCCTCAGCTCTCTTGTTGCTGGAGGGCAGTTATCTCAGGGTTCCAATT
TATCCATTAATACCAACCAAAACATCAGCATCAAGTCCGAACCGATTTCA
CCTCCTCGGGATCGTATGACCCCATCGGGCTTCCAGCAGCAGCAGCAGCA
GCAGCAGCAGCAGCAGCCGCCGCCACCACCGCAGCCCCAGCCACAACCCC
CGCAGCCCCAGCCCCGACAGGAAATGGGGCGCTCCCCTGTGGACAGTCTG
AGCAGCTCTAGTAGCTCCTATGATGGCAGTGATCGGGAGGATCCACGGGG
CGACTTCCATTCTCCAATTGTGCTTGGCCGACCCCCAAACACTGAGGACA
GAGAAAGCCCTTCTGTAAAGCGAATGAGGATGGACGCGTGGGTGACCTAAgaattc

Amino Acid Sequence

MDYKDDDDKGSPEFMGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELS
VLCDCEIALIIFNSSNKLFQYASTDMDKVLLKYTEYNEPHESRTNSDIVE
ALNKKEHRGCDSPDPDTSYVLTPHTEEKYKKINEEFDNMMRNHKIAPGLP
PQNFSMSVTVPVTSPNALSYTNPGSSLVSPSLAASSTLTDSSMLSPPQTT
LHRNVSPGAPQRPPSTGNAGGMLSTTDLTVPNGAGSSPVGNGFVNSRASP
NLIGATGANSLGKVMPTKSPPPPGGGNLGMNSRKPDLRVVIPPSSKGMMP
PLNTQRISSSQATQPLATPVVSVTTPSLPPQGLVYSAMPTAYNTDYSLTS
ADLSALQGFNSPGMLSLGQVSAWQQHHLGQAALSSLVAGGQLSQGSNLSI
NTNQNISIKSEPISPPRDRMTPSGFQQQQQQQQQQQPPPPPQPQPQPPQP
QPRQEMGRSPVDSLSSSSSSYDGSDREDPRGDFHSPIVLGRPPNTEDRES
PSVKRMRMDAWVT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5544
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *N.B. Difference in cloned sequence compared with NM_005587 CDS:; Deletion of nucleotides 865-888 (residues S289-L296) – Same region deleted in majority of EST sequences.; c891t (silent N297); ; Estimated MW of expressed protein : 55461 Daltons