cDNA Clone MEF2D

• Plasmid: pGEX6P-1-MEF2D
• Parent Plasmid: pGEX6P1
• Expressed: GST-MEF2D
• Genbank: NM_005920
• Tag: GST
• Species: Human

Sequence of Insert

gaattcATGGGGAGGAAAAAGATTCAGATCCAGCGAATCACCGACGAGCGGAACCGACAGGTGACTTTCACCAAGCGGAAGTTTGGCCTGATGAAGAAGGCGTATGAGCTGAGCGTGCTATGTGACTGCGAGATCGCACTCATCATCTTCAACCACTCCAACAAGCTGTTCCAGTACGCCAGCACCGACATGGACAAGGTGCTGCTCAAGTACACGGAGTACAATGAGCCACACGAGAGCCGCACCAACGCCGACATCATCGAGACCCTGAGGAAGAAGGGCTTCAATGGCTGCGACAGCCCCGAG
CCCGACGGGGAGGACTCGCTGGAACAGAGCCCCCTGCTGGAGGACAAGTA
CCGACGCGCCAGCGAGGAGCTCGACGGGCTCTTCCGGCGCTATGGGTCAA
CTGTCCCGGCCCCCAACTTTGCCATGCCTGTCACGGTGCCCGTGTCCAAT
CAGAGCTCACTGCAGTTCAGCAATCCCAGCGGCTCCCTGGTCACCCCTTC
CCTGGTGACATCATCCCTCACGGACCCGCGGCTCCTGTCCCCCCAGCAGC
CAGCACTACAGAGGAACAGTGTGTCTCCTGGCCTGCCCCAGCGGCCAGCT
AGTGCGGGGGCCATGCTGGGGGGTGACCTGAACAGTGCTAACGGAGCCTG
CCCCAGCCCTGTTGGGAATGGCTACGTCAGTGCTCGGGCTTCCCCTGGCC
TCCTCCCTGTGGCCAATGGCAACAGCCTAAACAAGGTCATCCCTGCCAAG
TCTCCGCCCCCACCTACCCACAGCACCCAGCTTGGAGCCCCCAGCCGCAA
GCCCGACCTGCGAGTCATCACTTCCCAGGCAGGAAAGGGGTTAATGCATC
ACTTGACTGAGGACCATTTAGATCTGAACAATGCCCAGCGCCTTGGGGTC
TCCCAGTCTACTCATTCGCTCACCACCCCAGTGGTTTCTGTGGCAACGCC
GAGTTTACTCAGCCAGGGCCTCCCCTTCTCTTCCATGCCCACTGCCTACA
ACACAGATTACCAGTTGACCAGTGCAGAGCTCTCCTCCTTACCAGCCTTT
AGTTCACCTGGGGGGCTGTCGCTAGGCAATGTCACTGCCTGGCAACAGCC
ACAGCAGCCCCAGCAGCCGCAGCAGCCACAGCCTCCACAGCAGCAGCCAC
CGCAGCCACAGCAGCCACAGCCACAGCAGCCTCAGCAGCCGCAACAGCCA
CCTCAGCAACAGTCCCACCTGGTCCCTGTATCTCTCAGCAACCTCATCCC
GGGCAGCCCCCTGCCCCACGTGGGTGCTGCCCTCACAGTCACCACCCACC
CCCACATCAGCATCAAGTCAGAACCGGTGTCCCCAAGCCGTGAGCGCAGC
CCTGCGCCTCCCCCTCCAGCTGTGTTCCCAGCTGCCCGCCCTGAGCCTGG
CGATGGTCTCAGCAGCCCAGCCGGGGGATCCTATGAGACGGGAGACCGGG
ATGACGGACGGGGGGACTTCGGGCCCACACTGGGCCTGCTGCGCCCAGCC
CCAGAGCCCGAGGCTGAGGGCTCAGCTGTGAAGAGGATGCGGCTTGATAC
CTGGACATTAAAGTGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMGRKKIQIQRITDERN
RQVTFTKRKFGLMKKAYELSVLCDCEIALIIFNHSNKLFQYASTDMDKVL
LKYTEYNEPHESRTNADIIETLRKKGFNGCDSPEPDGEDSLEQSPLLEDK
YRRASEELDGLFRRYGSTVPAPNFAMPVTVPVSNQSSLQFSNPSGSLVTP
SLVTSSLTDPRLLSPQQPALQRNSVSPGLPQRPASAGAMLGGDLNSANGA
CPSPVGNGYVSARASPGLLPVANGNSLNKVIPAKSPPPPTHSTQLGAPSR
KPDLRVITSQAGKGLMHHLTEDHLDLNNAQRLGVSQSTHSLTTPVVSVAT
PSLLSQGLPFSSMPTAYNTDYQLTSAELSSLPAFSSPGGLSLGNVTAWQQ
PQQPQQPQQPQPPQQQPPQPQQPQPQQPQQPQQPPQQQSHLVPVSLSNLI
PGSPLPHVGAALTVTTHPHISIKSEPVSPSRERSPAPPPPAVFPAARPEP
GDGLSSPAGGSYETGDRDDGRGDFGPTLGLLRPAPEPEAEGSAVKRMRLD
TWTLK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5505
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; N.B. Sequence difference compared with NM_005920 CDS:; T1509c (silent P503)