cDNA Clone RAPGAPII

• Plasmid: pEGFP-GLY-RAPGAPII
• Parent Plasmid: pEGFP Gly
• Expressed: GFP-RAPGAPII
• Genbank: CAI16255*
• Tag: GFP
• Species: Human

Sequence of Insert

GAATTCCGCACAGCTGCGGCCCGCGGTGCCACCCGGCCGTCCGCGGAGGG
GCTCGCTGCCCGCGGGGGCCAGCTGGCAGAACACAGATCTATTTGAGATG
ATTGAGAAGATGCAGGGAAGCAGGATGGATGAACAACGCTGCTCCTTCCC
GCCGCCCCTCAAAACAGAGGAGGACTACATTCCATACCCGAGCGTGCACG
AGGTCTTGGGGCGAGAAGGACCCTTCCCCCTCATCCTGCTGCCCCAGTTT
GGGGGCTACTGGATTGAGGGCACCAACCACGAAATCACCAGCATCCCCGA
GACAGAGCCACTGCAGTCGCCCACAACCAAGGTGAAGCTCGAGTGCAACC
CCACAGCCCGCATCTACCGGAAGCACTTTCTCGGCAAGGAGCATTTCAAT
TACTACTCACTGGACGCTGCCCTCGGCCACCTTGTCTTCTCACTCAAGTA
CGATGTCATCGGGGACCAAGAGCACCTGCGGCTGCTGCTCAGGACCAAGT
GCCGGACATACCATGATGTCATCCCCATCTCCTGCCTCACCGAGTTCCCT
AATGTTGTCCAGATGGCAAAGTTGGTGTGTGAAGACGTCAATGTGGATCG
GTTCTATCCTGTGCTCTACCCCAAGGCTTCCCGGCTCATCGTCACCTTTG
ACGAGCATGTCATCAGCAATAACTTCAAGTTTGGCGTCATTTATCAGAAG
CTTGGGCAGACCTCCGAGGAAGAACTCTTCAGCACCAATGAGGAAAGTCC
CGCTTTCGTGGAGTTCCTTGAATTTCTTGGCCAGAAGGTCAAACTGCAGG
ACTTTAAGGGGTTCCGAGGAGGCCTGGACGTGACCCACGGGCAGACGGGG
ACCGAATCTGTGTACTGCAACTTCCGCAACAAGGAGATCATGTTTCACGT
GTCCACCAAGCTGCCATACACGGAAGGGGACGCCCAGCAGTTGCAGCGGA
AGCGGCACATCGGGAACGACATCGTGGCTGTGGTCTTCCAGGATGAAAAC
ACTCCTTTCGTGCCCGACATGATCGCGTCCAACTTCCTGCATGCCTACGT
CGTGGTGCAGGCTGAGGGCGGGGGCCCTGATGGCCCCCTCTACAAGGTCT
CTGTCACTGCAAGAGATGATGTGCCCTTCTTTGGACCCCCCCTCCCGGAC
CCCGCTGTGTTCAGGAAGGGGCCTGAGTTCCAGGAATTTTTGCTGACAAA
GCTGATCAATGCTGAATATGCCTGCTACAAGGCAGAGAAGTTTGCCAAAC
TGGAGGAGCGGACGCGGGCCGCCCTCCTGGAGACGCTCTATGAGGAACTA
CACATCCACAGCCAGTCCATGATGGGCTTGGGCGGCGACGAGGACAAGAT
GGAGAATGGCAGTGGGGGCGGCGGCTTCTTTGAGTCTTTCAAGCGGGTCA
TCCGGAGCCGCAGCCAGTCCATGGATGCCATGGGGCTGAGCAACAAGAAG
CCCAACACCGTGTCCACCAGCCACAGCGGGAGCTTCGCGCCCAACAACCC
CGACCTGGCCAAGGCGGCTGGAATATCACTGATTGTCCCTGGGAAGAGCC
CCACGAGGAAGAAGGCGGGCCCGTTCGGCTCCCGCCGCAGCGCCGCCATT
GGCATCGAGAACATACAGGAGGTGCAGGAGAAGAGGGAGGCCCCTCCGGC
TGGTCAGAAGACCCCAGACAGCGGGCACGTCTCACAGGAGCCCAAGTCGG
AGAACTCATCCACTCAGAGCTCCCCAGAGATGCCCACGACCAAGAACAGA
GCGGAGACCGCAGCGCAGAGAGCAGAGGCGCTCAAGGACTTCTCCCGCTC
CTCGTCCAGTGCCAGCAGCTTCGCCAGCGTGGTGGAGGAGACGGAGGGTG
TGGACGGAGAGGACACAGGCCTGGAGAGCGTGTCATCCTCAGGAACACCC
CACAAGCGGGACTCCTTCATCTATAGCACGTGGCTGGAGGACAGTGTCAG
CACCACTAGTGGGGGCAGCTCCCCAGGCCCCTCTCGATCACCCCACCCAG
ACGCCGGCAAGTTGGGGGACCCTGCGTGTCCCGAGATCAAGATCCAGCTG
GAAGCATCTGAGCAGCACATGCCCCAGCTGGGCTGTTAGGGATCC

Amino Acid Sequence

…MDELYKYSDLSGGGGSNSAQLRPAVPPGRPRRGSLPAGASWQNTDLFEMIEKMQGSRMDEQRCSFPPPLKTEEDYIPYPSVHEVLGREGPFPLILLPQFGGYWIEGTNHEITSIPETEPLQSPTTKVKLECNPTARIYRKHFLGKEHFNYYSLDAALGHLVFSLKYDVIGDQEHLRLLLRTKCRTYHDVIPISCLTEFPNVVQMAKLVCEDVNVDRFYPVLYPKASRLIVTFDEHVISNNFKFGVIYQKLGQTSEEELFSTNEESPAFVEFLEFLGQKVKLQDFKGFRGGLDVTHGQTGTESVYCNFRNKEIMFHVSTKLPYTEGDAQQLQRKRHIGNDIVAVVFQDENTPFVPDMIASNFLHAYVVVQAEGGGPDGPLYKVSVTARDDVPFFGPPLPDPAVFRKGPEFQEFLLTKLINAEYACYKAEKFAKLEERTRAALLETLYEELHIHSQSMMGLGGDEDKMENGSGGGGFFESFKRVIRSRSQSMDAMGLSNKKPNTVSTSHSGSFAPNNPDLAKAAGISLIVPGKSPTRKKAGPFGSRRSAAIGIENIQEVQEKREAPPAGQKTPDSGHVSQEPKSENSSTQSSPEMPTTKNRAETAAQRAEALKDFSRSSSSASSFASVVEETEGVDGEDTGLESVSSSGTPHKRDSFIYSTWLEDSVSTTSGGSSPGPSRSPHPDAGKLGDPACPEIKIQLEASEQHMPQLGC*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU5418
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *N.B. Residue sequence differences compared with CAI16255 sequence:; ; S521A; insertion of Q residue after aa310 ; S529A (residue 530 in cloned sequence); S545A (residue 546 in cloned sequence) ; ; All of the above derive from HA-RAPGAPII template provided by D. Cantrell's lab.